Protein Assays
BIOHIPPO h/m CD36-sh-GFP/Luc
h/m CD36-sh-GFP/Luc shRNA lentiviral vector expressing GFP/Luc with selection for studies in Human/Mouse Each vial provides a nominal titer of 2x10E6 transducing units TU Validated h/m CD36 shRNA Lentivirus High-titer shRNA lentiviral particles specific for human/mouse CD36 molecule (CD36) The shRNA has been validated to meet or exceed 70% CD36 knockdown efficiency using a specific fluorescence-based method that is more rapid and reliable than qPCR The shRNA lentivirus is ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation and ideal for transducing difficult-to-transfect cells including thawed and/or primary cells Order a shRNA lentivirus set and receive lentivirus produced from mix of 2 independent shRNAs validated to knockdown the target gene ( 70%) plus control lentivirus produced from mix of 2 scrambled shRNA constructs
BIOHIPPO CMV-Luc-RFP-BSD
CMV-Luc-RFP-BSD is a fluorescence and luciferase dual imaging reporter lentivirus based on the LFV-V1a vector layout under control of the CMV promoter containing a Luc-T2A-RFP-P2A-BSD expression cassette and Blasticidin resistance marker This construct co expresses luciferase and fluorescent reporter proteins to enable correlated bioluminescent and fluorescent readouts in the same cell population Each vial provides a nominal titer of 2x10E6 transducing units TU The lentiviral particles are supplied ready to use for stable gene delivery to mammalian cells and can facilitate generation of long term reporter cell lines They are well suited for in vitro cell based assays in vivo optical imaging high content screening and mechanism of action studies This product is intended for research use only and is not for diagnostic or therapeutic procedures
BIOHIPPO CMV-RFP-Puro-Luc
CMV-RFP-Puro-Luc is a fluorescence and luciferase dual imaging reporter lentivirus based on the LFV-V1b vector layout under control of the CMV promoter containing a RFP-T2A-Puro-P2A-Luc expression cassette and Puromycin resistance marker This construct co expresses luciferase and fluorescent reporter proteins to enable correlated bioluminescent and fluorescent readouts in the same cell population Each vial provides a nominal titer of 2x10E6 transducing units TU The lentiviral particles are supplied ready to use for stable gene delivery to mammalian cells and can facilitate generation of long term reporter cell lines They are well suited for in vitro cell based assays in vivo optical imaging high content screening and mechanism of action studies This product is intended for research use only and is not for diagnostic or therapeutic procedures
Vector Biolabs NFAT-Luc Adenovirus (Ad-NFAT-Luc)
Ready to use in vivo grade AAV and Adenovirus Ready to use in vivo grade AAV and Adenovirus pre synthesized DNA vector High titer viral vectors 1/EA
Cayman Chemical FIrefly LucIferase mRNACap 1mg
An mRNA for the expression of luciferase; encapsulation in LNPs can be used for mRNA delivery and expression of luciferase protein in vitro or in vivo
Sigma Aldrich Fine Chemicals Biosciences ATP Cell Viability Lucifer
Because ATP is an indicator of metabolically active cells the number of viable cells can be assessed based on the amount of ATP available. The ATP Cell Viability Luciferase Assay offers a highly sensitive homogenous assay for quantifying ATP in cell cultures. This kit takes advantage of firefly luciferases use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available in cell cultures. The sensitive assay procedure involves a single addition of ATP detection cocktail directly to cells cultured in a serum-supplemented medium. No cell washing medium removal and multiple pipetting are required. The kit can be used to detect as little as a single cell or 0.01 picomoles of ATP. The signal produced is linear within 6 orders of magnitude. By relating the amount of ATP to the number of viable cells the assay has wide applications ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity.The ATP C
BIOHIPPO NOTCH-RFP-Luc-BSD LV
NOTCH-RFP-Luc-BSD is a transcription factor reporter lentiviral vector designed to monitor NOTCH signaling pathway in living cells The construct carries a RFP and luciferase dual reporter under the control of pathway responsive elements and includes a blasticidin selection marker to generate stable cell pools The lentivirus is provided as a ready to use VSV G pseudotyped preparation for efficient gene delivery and stable genomic integration Each vial provides a nominal titer of 5x10E6 transducing units TU Typical applications include pathway activation studies assay development compound screening and mechanism of action research The system is suitable for human and mouse cells and can be read using standard fluorescence microscopy flow cytometry or luciferase assays depending on the reporter gene This product is intended for research use only and is not for diagnostic or therapeutic procedures
Signosis Inc SMAD/TGFbeta Luciferase Reporter NIH/3T3 Stable Cell Line SL-0030
SMAD 2/3 Responsive Luciferase Reporter NIH/3T3 Stable Cell Line is derived from mouse fibroblast,and stably express firefly luciferase reporter gene under the control of SMAD 2/3 response element. This cell line is an ideal cellular model for monitoring the activation of TGFbeta Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
BIOHIPPO CMV-RFP-BSD-Luc
CMV-RFP-BSD-Luc is a fluorescence and luciferase dual imaging reporter lentivirus based on the LFV-V1b vector layout under control of the CMV promoter containing a RFP-T2A-BSD-P2A-Luc expression cassette and Blasticidin resistance marker This construct co expresses luciferase and fluorescent reporter proteins to enable correlated bioluminescent and fluorescent readouts in the same cell population Each vial provides a nominal titer of 2x10E6 transducing units TU The lentiviral particles are supplied ready to use for stable gene delivery to mammalian cells and can facilitate generation of long term reporter cell lines They are well suited for in vitro cell based assays in vivo optical imaging high content screening and mechanism of action studies This product is intended for research use only and is not for diagnostic or therapeutic procedures