Test
Purify GTPases and assay activation levels using specific GST-tagged protein-binding domains (PBD), GSH-agarose beads and anti-GTPase antibodies.
Purify GTPases and assay activation levels using specific GST-tagged protein-binding domains (PBD), GSH-agarose beads and anti-GTPase antibodies.
Purify GTPases and assay activation levels using specific GST-tagged protein-binding domains (PBD), GSH-agarose beads and anti-GTPase antibodies.
Purify GTPases and assay activation levels using specific GST-tagged protein-binding domains (PBD), GSH-agarose beads and anti-GTPase antibodies.
Purify GTPases and assay activation levels using specific GST-tagged protein-binding domains (PBD), GSH-agarose beads and anti-GTPase antibodies.
Purify GTPases and assay activation levels using specific GST-tagged protein-binding domains (PBD), GSH-agarose beads and anti-GTPase antibodies.
For quantitative determination of ATPase or GTPase activity and high-throughput screen for their inhibitors. Key Features: High sensitivity. Detection of 0.007 U/L ATPase or GTPase activity. Fast and convenient: single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme activity within 30 minutes. Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: OD620nm. Samples: ATPase and GTPase. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.007 U/L.
Cell Biolabs’ RhoA Activation Assay Kit provides a simple and fast tool to monitor the activation of RhoA. The kit utilizes easily identifiable Rhotekin RBD Agarose beads (pink in color) to selectively isolate and pull-down the active form of Rho from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rho is detected by western blot analysis using an Anti-RhoA specific antibody. Each kit includes an Immunoblot Positive Control and provides sufficient quantities to perform 20 assays.
g-lisa cdc42 activation assay 24
Cell Biolabs’ RhoA/Rac1/Cdc42 Activation Assay Combo Kit provides a simple and fast tool to monitor the activation of RhoA, Rac1 and Cdc42. The kit utilizes easily identifiable Agarose beads (pink in color) to selectively isolate and pull-down the active form of Rho, Rac, or Cdc42 from purified samples or endogenous lysates. Subsequently, the precipitated GTP-form is detected by western blot analysis using an Anti-RhoA , Anti-Rac1, or Anti-Cdc42 specific antibody. Each kit includes an Immunoblot Positive Control and provides sufficient quantities to perform 10 assays of each GTPase target.
Purified anti-RhoA [A18230D]; Isotype: Mouse IgG1, κ; Reactivity: Human, Mouse; Apps: WB, ICC; Size: 25 μg
Combo RhoA/Rac1/Cdc42 Activation Assay Kit KIT 3 x 10 assays
NC2606601 HTS KINESIN ATPASE ENDPOINT AS
Purified anti-RhoA [A18230D]; Isotype: Mouse IgG1, κ; Reactivity: Human, Mouse; Apps: WB, ICC; Size: 100 μg