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Specific assay kits designed measure the activation levels of small, active GTPases by isolating them via their specific downstream effectors. Kits employ molecular methods including pull-down affinity purification, electrophoresis, and western blotting.
Cell Biolabs’ RhoA Activation Assay Kit provides a simple and fast tool to monitor the activation of RhoA. The kit utilizes easily identifiable Rhotekin RBD Agarose beads (pink in color) to selectively isolate and pull-down the active form of Rho from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rho is detected by western blot analysis using an Anti-RhoA specific antibody. Each kit includes an Immunoblot Positive Control and provides sufficient quantities to perform 20 assays.
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