Antibody and Dye Based Assays
Promega ADCC Reporter Bioassay, Complete (WIL2-S)
Provides a biologically relevant and specific mechanism-of-action-based measure of ADCC without the complex workflow and variability inherent in primary cell-based assays. The bioassay also can be used to quantify effects of antibody glycosylation on Fc effector function.
Promega ADCC Reporter Bioassay, F Variant, Core Kit
Provides a biologically relevant and specific mechanism-of-action-based measure of ADCC without the complex workflow and variability inherent in primary cell-based assays. The bioassay also can be used to quantify effects of antibody glycosylation on Fc effector function.
Promega pHAb Amine Reactive Dye, 1 x 250microg
pHAb Amine Reactive Dye exhibits very low fluorescence at pH > 7 and much higher fluorescence as the pH of the solution becomes acidic. pHAb Dyes are designed specifically for antibody labeling.
Promega ADCC Bioassay Effector Cells, F Variant, Propagation Model
Allows propagation and banking of the ADCC Bioassay, F Variant, Effector Cells without the complex workflow and variability inherent in primary cell-based assays. The bioassay also can be used to quantify effects of antibody glycosylation on Fc effector function.
Abcam Phalloidin-iFluor 488 Reagent
Phalloidin-iFluor 488 Reagent
The product is subject to the following: Abcam Restricted Use Statement
Biotium Mix-n-Stain™ CF488A Antibody Labeling Kit, 1x(50-100ug) labeling
Mix-n-Stain™ Antibody Labeling Kits are a revolutionary antibody labeling technology that allows you to label your antibody with one of our superior CF dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Choose from kit sizes to label 5-20 ug, 20-50 ug, or 50-100 ug of your antibody. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm.
Abcam PE / R-Phycoerythrin Conjugation Kit - Lightning-Link, 600UG
The Abcam PE/R-Phycoerythrin Conjugation Kit (ab102918) offers several standout features: -Rapid Conjugation: achieve PE/R-Phycoerythrin labeling in under 4 hours with just 30 seconds of hands-on time. -High Efficiency: ensures 100% antibody recovery, preserving the integrity and value of your antibodies. -Versatility: suitable for conjugating antibodies, proteins, and peptides, and the labeled antibodies can be used immediately in applications such as Western Blotting (WB), ELISA, and Immunohistochemistry (IHC) without further purification. - Confidence: cited in over 50 publications
The product is subject to the following: Abcam Restricted Use Statement
Biotium Mucin 5AC (MUC5AC)(45M1), CF488A conjugate, 0.1mg/mL
This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. The epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specifi
Biotium CD50 (ICAM-3)(186-2G9), CF488A conjugate, 0.1mg/mL
Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium BCL-10(BL10/411), CF488A conjugate, 0.1mg/mL
B-cell lymphoma/leukemia 10 (BCL10) is a signaling protein that promotes apoptosis, pro-caspase-9 maturation, and activation of NF-kappa-B via NIK and IKK. BCL10 contains an N-terminal caspase recruitment domain (CARD), which is found in a number of apoptotic regulatory molecules. BCL10 is involved in the adaptive immune response, and may be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. A BCL10 translocation is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma)Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium CD81 / TAPA-1 (1.3.3.22), CF488A conjugate, 0.1mg/mL
This antibody recognizes a protein of 26 kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightne
Biotium CD50 (ICAM-3)(186-2G9), CF488A conjugate, 0.1mg/mL
Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium Chromogranin A(CHGA/413), CF488A conjugate, 0.1mg/mL
Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes