General Cell Based Assays
Eurofins Discoverx PathHunter eXpress BAI3 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0547E2ACP1L)
The PathHunter BAI3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of BAI3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BAI3 and EA-tagged β-Arrestin-2. Activation of BAI3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human & Mouse Total c-Src Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total c-Src Detection Kit, 10,000 Assay Points
Eurofins Discoverx PathHunter eXpress OPRD1 Activated GPCR Internalization Assay, 2pl Kit. (93-0673E3CP0M)
The PathHunter OPRD1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OPRD1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged OPRD1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting OPRD1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human A2M ASY 25 PL
The Meso Scale Discovery U-PLEX Human A2M Assay is a multiplex immunoassay kit that enables simultaneous quantitative measurement of Alpha-2-Macroglobulin (A2M) in human serum plasma and cell culture supernatants This electrochemiluminescent assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and accurate detection of A2M levels down to sub-pg/mL concentrations The assay requires 50 L sample volume and can be read on MSD instrument platforms This product provides a simple rapid way to quantify A2M levels which can aid research into inflammatory conditions immune function and proteolytic regulation
Revvity Health Sciences Inc HTRF Human Total RIPK1 Detection Kit, 500 Assay Points
HTRF Human Total RIPK1 Detection Kit, 500 Assay Points
Abcam Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)
Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Collagen Degradation/Zymography Assay Kit (Fluorometric)
Collagen Degradation/Zymography Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress FGFR4-β-Klotho Functional Assay, 10pl Kit. (93-1060E3CP0L)
The PathHunter eXpress FGFR4-β-Klotho Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated FGFR4 and β-Klotho dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FGFR4 and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, β-Klotho. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
FUJIFILM Biosciences ADRA Kit
Amino acid Derivative Reactivity Assay ADRA is an alternative method of skin sensitization test to animal testing ADRA significantly reduces the number of chemical substances used as a reaction reagent by using a novel amino acid derivative having a naphthalene ring capable of high sensitivity detection by ultraviolet rays Also skin sensitization tests are possible even at about 1/100 by conventional methods It is possible to evaluate skin sensitization with high accuracy without causing chemical substances to precipitate in the reaction solution It evaluates whether a chemical substance provokes an allergic reaction in the skin without using animal models Reagent kit for performing ADRA as described in OECD TG 442C
Eurofins Discoverx PathHunter eXpress mCCR9 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0734E2MCP2M)
The PathHunter mCCR9 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL25) based activation of mCCR9 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR9 and EA-tagged β-Arrestin-2. Activation of mCCR9 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical AMPK activator, Purity: ≥95%, CAS: 849727-81-7, 10 mg
An indirect activator of AMPK (EC50 = 11.7 μM); stimulates glucose uptake in L6 myocytes and promotes phosphorylation of ACC, a known target of AMPK; decreases blood glucose concentrations in Zucker diabetic rats, demonstrating its utility in whole animals; inhibits the growth of multiple myeloma cells
Eurofins Discoverx PathHunter eXpressNR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0323E2CP0M)
The PathHunter MLNR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. motilin) based activation of MLNR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MLNR and EA-tagged β-Arrestin-2. Activation of MLNR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress MCHR1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0055E2CP2L)
The cAMP Hunter MCHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. melanin concentrating hormone) based activation of MCHR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MCHR1, a naturally Gi/Go coupled GPCR. Upon activation of MCHR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCKBR CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0191E2CP0L)
The PathHunter CCKBR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. cholecystokinin) based activation of CCKBR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCKBR and EA-tagged β-Arrestin-2. Activation of CCKBR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.