General Cell Based Assays
Eurofins Discoverx PathHunter eXpress HRH1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0503E2CP2M)
The PathHunter HRH1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH1 and EA-tagged β-Arrestin-2. Activation of HRH1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GIPR CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-1095E2CP2L)
The PathHunter GIPR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GIP) based activation of GIPR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GIPR and EA-tagged β-Arrestin-2. Activation of GIPR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress FPR3 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0171E2CP2M)
The cAMP Hunter FPR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. humanin) based activation of FPR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FPR3, a naturally Gi/Go coupled GPCR. Upon activation of FPR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
AURORA BIOLABS LLC Cov-19 NP Rabbit Binding Assay, 384 rxn
SARS-CoV-2 Nucleocapsid protein (NP) is one of the core components of SARS-CoV-2 virus. It forms a complex with viral genomic RNA in a helical symmetrical structure and plays a key role in the process of virus replication and assembly. Since NP is abundantly expressed during infection, it can be used as an important diagnostic marker for COVID-19 and also can be used as a potential drug target or developing vaccines. Terbium-labeled anti-Tag1 antibody serves as fluorescence donor, that binds to the His-Tagged NP. If a test rabbit antibody binds to NP, fluorescence- labeled anti-rabbit antibody (fluorescence acceptor) will be brought in close proximity with the fluorescence donor. Excitation of Terbium (340 nm) generates fluorescence resonance energy transfer (FRET) to the fluorescence-labeled acceptor, which consequently fluoresces at 665 nm (figure below). Thus, the test antibody binding to NP can be quantitively measured by calculation of the fluorescent ratio of 665 nm/620 nm.
Eurofins Discoverx PathHunter eXpress INSRb Functional Assay, 10-Plate Kit. (93-0466E3CP16L)
The PathHunter eXpress INSRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated INSRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged INSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery RPLEX HUM Neurotrophin3 ASY
The R-PLEX Human Neurotrophin-3 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures human neurotrophin-3 (NT-3) protein levels across samples This assay utilizes MSD s electrochemiluminescent detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The kit includes a 96-well plate pre-coated with capture antibodies specific for NT-3 detection antibodies calibrators and reagents required to perform the assay Quantitative results can be obtained in as little as 3 5 hours This assay is for research use only
Eurofins Discoverx PathHunter eXpress FGR Activity Assay, 2-Plate Kit. (93-0884E3CP16M)
The PathHunter eXpress FGR Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the FGR kinase domain. The cell line is engineered to express the catalytic domain from FGR fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the FGR catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Citrulline Assay Kit (Fluorometric)
Citrulline Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Enzo Life Sciences DPPIV/CD26 Assay kit for biological samples (1x96wells)
Because DPPIV/CD26 protein levels may not accurately reflect the levels of active DPPIV enzyme, it is useful to measure DPPIV activity rather than performing DPPIV immunoassay. The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered .½-volume NBS white microplate (Prod. No. BML-KI571)
Sigma Aldrich Fine Chemicals Biosciences Pyruvate Assay Kit suffici
Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis to fatty acids or energy through acetyl-CoA to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.
Eurofins Discoverx PathHunter eXpress GPR35 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0942E2CP2M)
The PathHunter GPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR35 and EA-tagged β-Arrestin-2. Activation of GPR35 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human MMP-3 ASY
The R-PLEX Human MMP-3 Assay Kit from Meso Scale Discovery is used to quantitatively measure MMP-3 levels in human serum plasma saliva urine cell culture supernatant and other biological samples It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity a broad dynamic range and requires low sample volume The assay has high specificity for active MMP-3 with no cross-reactivity to pro-MMP-3 The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer diluent and calibrator It can detect MMP-3 levels between 0 03-1000 ng/mL
Revvity Health Sciences Inc AlphaScreen Mouse IgG Detection Kit, 50,000 Assay Points
AlphaScreen Mouse IgG Detection Kit, 50,000 Assay Points