General Cell Based Assays
Revvity Health Sciences Inc HTRF Human Total c-RAF Detection Kit, 10,000 Assay Points
HTRF Human Total c-RAF Detection Kit, 10,000 Assay Points
Eurofins Discoverx PathHunter eXpress OXTR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0261E2CP2M)
The PathHunter OXTR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. oxytocin) based activation of OXTR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OXTR and EA-tagged β-Arrestin-2. Activation of OXTR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CALCRL-RAMP2 (AM1) CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0270E2CP0M)
The PathHunter CALCRL-RAMP2 (AM1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. adrenomedullin) based activation of CALCRL GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCRL and EA-tagged β-Arrestin-2, along with untagged modulating protein RAMP2. Activation of CALCRL stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCNR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0472E2MCP0L)
The PathHunter mCNR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of mCNR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCNR2 and EA-tagged β-Arrestin-2. Activation of mCNR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter GHSR Bioassay Kit, 2-Plate. (93-0242Y3-00141)
The PathHunter GHSR Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay including cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure long-term assay reproducibility, and faster implementation from characterization to lot release. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress GCGR CHO-K1 β-Arrestin-1 GPCR Assay, 2-Plate Kit. (93-0979E2CP2M)
The PathHunter GCGR (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. glucagon) based activation of GCGR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GCGR and EA-tagged β-Arrestin-1. Activation of GCGR stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR65 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0428E2ACP1L)
The PathHunter GPR65 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR65 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR65 and EA-tagged β-Arrestin-2. Activation of GPR65 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
TOPOGEN INC ICE Assay Kit (No AB)
TopoGEN has developed and published a method for detecting covalent topo/genomic DNA complexes in live cells that is widely utilized in the field. The method, called the ICE Assay (In vivo Complex of Enzyme) originally required a CsCl gradient to purify genomic DNA and specific topo antibodies to detect trapped topo protein in the DNA fraction. This method, while powerful, is slow, labor intense and requires large expensive equipment. We have modified the technique to allow direct isolation of DNA/topo complexes in a single step, omitting the CsCl gradient step and rapidly increasing the throughput of the method. The ICE Assay can be carried out in a day and a half, and the analysis can be conducted on virtually any cell or tissue system as long as proper controls are performed and mono-specific antibody reagents are used. Our new ICE method is rapid and convenient and can be used with any topoisomerase target (I, II, III).
Eurofins Discoverx PathHunter eXpress GRPR Activated GPCR Internalization Assay, 10pl Kit. (93-0667E3CP5L)
The PathHunter GRPR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GRPR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GRPR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GRPR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.