General Cell Based Assays
Eurofins Discoverx PathHunter Human T-Cell Receptor Activation Pathway Reporter Cell-Based Assay Kit (Jurkat)
The PathHunter Human T-Cell Receptor Activation Pathway NFAT Reporter Stable Cell Line Assay (Jurkat) measures induced expression levels of a tagged reporter protein. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human CCK2 (CCKBR) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human CCK2 (CCKBR) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CCKBR (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human MTNR1B Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human MTNR1B Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure MTNR1B (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery R-PLEX Human ADAM17 ASY
The R-PLEX Human ADAM17 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures active ADAM17 in human serum plasma and cell culture supernatants The assay utilizes MSD s electrochemiluminescent detection technology and provides high sensitivity a broad dynamic range and requires a low sample volume The kit includes a 96-well plate coated with capture antibody a detection antibody and calibrator The assay can detect ADAM17 levels between 1 5-1000 pg/mL and each kit provides sufficient reagents for 1 x 96-well plate This product enables the accurate quantitation of ADAM17 which plays a key role in regulating EGFR signaling
Meso Scale Discovery U-PLEX Human IL-1 ASY
The Meso Scale Discovery U-PLEX Human IL-1alpha Assay Kit is a multiplex immunoassay that measures IL-1alpha in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The assay can quantify IL-1alpha concentrations between 0 06 pg/mL to 10 000 pg/mL It includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels calibrators and an MSD read buffer The total assay time is 3 5 hours
Eurofins Discoverx PathHunter eXpress rTrkA-rP75 Functional Assay, 10-Plate Kit. (93-1014E3CP16L)
The PathHunter eXpress rTrkA-rP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rTrkA and rP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.