General Cell Based Assays
AdipoGen Insulin human ELISA Kit
Colorimetric Sandwich ELISA Assay. Detects human Insulin in serum, plasma and cell culture supernatant. Range 7.81 to 500pg/ml. Sensitivity 10 pg/ml. Samples Cell Culture Supernatant, Plasma, Serum. Glucose is the main fuel for the human body and blood glucose values are tightly regulated in healthy individuals. Regulation of glucose uptake in cells is performed by the hormone called insulin which is glucose-responsively secreted from pancreatic beta cells. When the glucose concentration in the blood is increased, insulin lowers it by increasing glucose uptake by muscle, liver and fat cells. Insulin is synthesized by beta cells of the pancreas in the form of a single chain of three peptides B, C and A in the order B-chain-C peptide-A-chain. The mature insulin consists of B-chain 30 amino acids and A-chain 21 amino acids linked by two inter-chain and one intra-chain disulfide bridge.
