General Cell Based Assays
Eurofins Discoverx PathHunter eXpress GALR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0228E2CP0M)
The PathHunter GALR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of GALR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GALR1 and EA-tagged β-Arrestin-2. Activation of GALR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TRHR CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0239E2CP0L)
The PathHunter TRHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. TRH) based activation of TRHR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TRHR and EA-tagged β-Arrestin-2. Activation of TRHR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTAFR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0236E2CP0M)
The PathHunter PTAFR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PAF) based activation of PTAFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTAFR and EA-tagged β-Arrestin-2. Activation of PTAFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Molecular Devices Assay Kit, Calcium 6, Evaluation, Fluorescence, 96/384/1536-well
The FLIPR Calcium 6 Evaluation Kit provides a homogeneous, fluorescence-based method to detect intracellular calcium changes in cell-based assays. It is formulated for use with compatible fluorescence plate readers and supports multiple microplate formats.
- Homogeneous fluorescence assay for intracellular calcium detection.
- Evaluation kit sized for three assay plates and three QF assay plates.
- Includes 3 vials of component A, 3 vials of component C for QF, and 1 bottle of assay buffer.
- Compatible with 96-well, 384-well, and 1536-well microplates.
- Designed for screening GPCRs, ion channels, and calcium-mediated signaling.
- Each reagent vial is sufficient for one plate, depending on dispenser dead volume.
Abcam Glucose Assay Kit - reducing agent compatible
Glucose Assay Kit - reducing agent compatible
The product is subject to the following: Abcam Restricted Use Statement
Abcam Magnesium Assay Kit
Magnesium Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam Glycogen Assay Kit
Glycogen Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
ILEX LIFE SCIENCES LLC QuickZyme Hydroxyproline Assay Kit, 5 x 96 Assays Bulk Pack, 96-well plate format, colorimetric assay for collagen analysis (conditioned media cells, cell extract, tissue, tissue extract samples)
The QuickZyme Hydroxyproline Assay Kit enables quantitative collagen analysis in all types of tissues, in a fast and simple 96-well plate format. This colorimetric assay does not need the drying step following acid hydrolysis of collagen for which often special equipment is needed. This assay is the only hydroxyproline-based assay that does not need this laborious step, resulting in a fast, sensitive, and easy assay. NOTE: This kit does not contain tubes or a protocol for collagen hydrolysis. If you need these items please order the QuickZyme Total Collagen Assay. Application: conditioned media cells, cell extracts, tissues, tissue extracts. Sensitivity: 2.4 µM (0.3 µg/ml). Assay-time: < 2 hours. Collagen is one of the main components of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Thus measurement of collagen production is important in many disease related studies.
Bioassay Systems QuantiChrom™ Magnesium Assay Kit. For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism.
For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL sample. Linear detection range 0.1 mg/dL (41 µM) to 3 mg/dL (1.2 mM) Mg2+ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. Assays can be directly performed in serum and urine samples. Method: OD500nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 0.1 mg/dL (41 µM).
Eurofins Discoverx PathHunter eXpress EGFR/EGFR Dimerization Assay, 2-Plate Kit. (93-0989E3CP5M)
The PathHunter EGFR/EGFR Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. EGF) induced dimerization of EGFR/EGFR receptors. This product uses EFC technology. The cell line included overexpresses EGFR target protein fused to PK alongside EGFR target protein fused to EA. Upon ligand-induced dimerization of EGFR/EGFR, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CMKLR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0313E2CP0M)
The PathHunter CMKLR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of CMKLR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CMKLR1 and EA-tagged β-Arrestin-2. Activation of CMKLR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.