General Cell Based Assays
Eurofins Discoverx PathHunter Human PPYR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PPYR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PPYR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical CelBased asy MyeloperoxId 25uL
A peptide MC1R antagonist (IC50 17 nM) selectively binds to MC1R over MC3R and MC5R (IC50s 3900 and 1000 nM respectively) as well as MC4R at 10 UM increases the latency to tail withdrawal in the tail-flick test in female but not male mice in a model of opioid-induced hyperalgesia at 7.5 UG/animal i.c.v. reduces protein levels of merlin in the cortex reduces the number of surviving neurons in the cortex and hippocampal CA2 region and increases the number of apoptotic cells in the cortex in rats 24 hours after injury in a model of controlled cortical impact-induced TBI at 1 nmol/animal i.c.v.
Eurofins Discoverx PathHunter eXpress ADRA2A CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0424E2CP0L)
The PathHunter ADRA2A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRA2A and EA-tagged β-Arrestin-2. Activation of ADRA2A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Insulin Lispro Bioassay Kit, 10pl. (93-0466Y3-00010)
The PathHunter Insulin Lispro Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses insulin or insulin lispro based activation of INSRb RTK receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged INSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress P2RY6 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0500E2CP19L)
The PathHunter P2RY6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY6 and EA-tagged β-Arrestin-2. Activation of P2RY6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 20 Reagent, 100. (93-0563R20A)
AssayComplete Cell Plating 20 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
BioVendor, LLC QuickZyme Sensitive Tissue Collagen Assay Kit 5 X 96-Assays
QuickZyme Sensitive Tissue Collagen Assay Kit, 5 X 96-Assays. Measure hydroxyproline in small tissue samples with low collagen levels! The ONLY hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate, enabling measurement in small samples with low hydroxyproline levels, such as liver. No drying of hydrolysate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay. Dynamic range: 6-300 ug/ml. Sensitivity: 2.5 ug/ml. Sample vol. 50-500 ul. Species independent. Measures total hydroxyproline. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all collagen types present without discriminating between types of collagen, procollagen, mature collagen and collagen degradation products. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. Collagen hydrolysis tubes & protocol ARE included. Ease-of-use: Equivalent to ELISA.
Meso Scale Discovery U-PLEX Human Nectin-4 ASY
The Meso Scale Discovery Human Nectin-4 Singleplex Assay Kit is a singleplex immunoassay that measures Nectin-4 levels in human serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity wide dynamic range and low sample volume requirements The assay requires 50 L of sample and can be read on Meso Scale Discovery SECTOR Imagers The assay has a typical lower limit of detection of 0 03 ng/mL and an upper limit of quantification of 2500 ng/mL This assay kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator and control
Eurofins Discoverx PathHunter Human F2RL3 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human F2RL3 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure F2RL3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human AVPR2 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human AVPR2 β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure AVPR2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ILEX LIFE SCIENCES LLC QuickZyme Human MMP-7 Activity Assay Kit, 96 Assays, 96-well plate format, immunoassay for active or total matrix metalloproteinase 7 (conditioned culture medium, serum, urine, tissue homogenates samples)
The QuickZyme Human MMP-7 Activity Assay is an enzyme activity immunoassay that enables you to specifically measure both endogenous active MMP-7 and total active MMP-7 (following activation of pro-MMP-7 on the plate with APMA). It can be used for the measurement of MMP-7 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine. This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity. ASSAY RANGE: 0.004 ¿ 16 ng/ml. SENSITIVITY: 30 pg/ml (3 h incubation); 4 pg/ml (25 h incubation). SYNONYMS: Matrix metalloproteinase-7, MMP7, Matrilysin, Matrilysin-1, Matrin, Pump-1 protease, Uterine metalloproteinase.
Meso Scale Discovery R-PLEX Rat β-NGF Assay, 5 plate size, Singleplex format, 96-well plate, Plasma and Serum sample types, Dynamic range 0.2 - 6000 pg/mL, LLOD 0.2 pg/mL, For research use only
R-PLEX Rat β-NGF Assay, 5 plate size, Singleplex format, 96-well plate, Plasma and Serum sample types, Dynamic range 0.2 - 6000 pg/mL, LLOD 0.2 pg/mL, For research use only
Indigo Biosciences INDIGO BIOSCIENCES INC Assay, FGFR1c/α-Klotho, Endocrine FGF Signaling, 1x 384-well
This product is an assay for Endocrine FGF Signaling.
- Uses proprietary human cells engineered to provide constitutive expression of Human Fibroblast Growth Factor Receptor 1, isoform 1 and Human Fibroblast Growth Factor Receptor 2, isoform 1.
- Incorporates a luciferase reporter gene functionally linked to tandem NFAT consensus response element sequences.
- Provides an all-inclusive cell-based reporter assay system.
- Includes Reporter Cells, optimized culture medium, heparin-supplemented culture medium, FGF-Acidic (physiological paracrine activator of FGFR1/2), Luciferase Detection Reagents, and a cell culture-ready assay plate.
- Kit materials are sufficient to perform assays in a single 384-well assay plate.
- Cryopreserved mammalian cells are provided and require storage at -80°C until rapid-thaw.
- Intended for research purposes only.
Medchemexpress LLC Phospholipid transfer protein, recombinant human (HEK293, C-terminal His tag) | >95.0% | 10 UG
Recombinant human phospholipid transfer protein (PLTP) produced in HEK293 cells with a C-terminal 10xHis tag. Supplied lyophilized for biochemical and cell-based functional assays; demonstrates activity in inhibiting LPS-induced TNF-α secretion in macrophage cells.
- Expressed in HEK293 cells.
- C-terminal 10xHis affinity tag.
- Purity greater than 95% by reducing SDS-PAGE.
- Predicted molecular mass 54.4 kDa; appears ~55-90 kDa by SDS-PAGE due to glycosylation.
- Functional activity: 5 μg/mL reduces LPS-induced TNF-α secretion in RAW 264.7 cells.
- Lyophilized formulation with trehalose and mannitol for stability.
- Store frozen; aliquot and keep at -20 °C or -80 °C for long-term storage.
- Available in small research quantities such as 10 μg and 50 μg.
Eurofins Discoverx PathHunter eXpress VIPR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0316E2CP0M)
The PathHunter VIPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. VIP) based activation of VIPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged VIPR1 and EA-tagged β-Arrestin-2. Activation of VIPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.