General Cell Based Assays
Eurofins Discoverx PathHunter eXpress GPR15 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0682E2CP1L)
The PathHunter GPR15 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR15 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR15 and EA-tagged β-Arrestin-2. Activation of GPR15 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
10X Genomics Inc GEM-X Flex Gene Expression Human n-plex, 64 samples
GEM-X Flex Gene Expression Human n-plex, 64 samples
Cayman Chemical NonradIoactIve IodID asy K 96
A B2-AR agonist selectively binds to B1- over B2-ARs (Kds 2.9 and 113 nM respectively) as well as B3-adrenergic B2 bradykinin NK1 and NK2 receptors up to 3 M induces cAMP accumulation in cultured human bronchial epithelial cells relaxes guinea pig tracheal strips precontracted with carbamoylcholine ovalbumin or histamine (pD2s 8.4 9.5 and 9.5 respectively) reverses histamine- and ovalbumin-induced bronchoconstriction in guinea pigs (ED50s 1 and 40 nmol/kg respectively)
Eurofins Discoverx PathHunter Human OPRM1 (Opioid mu) β-Arrestin-1 Cell-Based Assay Kit (U2OS)
The PathHunter Human OPRM1 (Opioid mu) β-Arrestin-1 Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure OPRM1 (GPCR) activity via recruitment of β-Arrestin-1. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human IL-21 (IL21R/IL2RG) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human IL-21 (IL21R/IL2RG) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IL21R/IL2RG. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human TSLP (IL7R/CRLF2) Dimerization Cell-Based Assay Kit (DLD1)
The PathHunter Human TSLP (IL7R/CRLF2) Stable Cell Line Dimerization Assay (DLD1) measures ligand-induced dimerization of IL7R/CRLF2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress mGIPR U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0846E3MCP5M)
The PathHunter mGIPR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GIP) based activation of mGIPR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGIPR and EA-tagged β-Arrestin-2. Activation of mGIPR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX AB38 4G8 ASY
The Meso Scale Discovery R-PLEX Human Abeta38 (4G8) Assay Kit is a multiplex immunoassay that measures Abeta38 in human serum plasma CSF and cell culture supernatants It uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a plate coated with a 4G8 antibody to capture Abeta38 It also includes detection antibodies standards buffers calibrators controls and substrate The kit is for research use only and not for use in diagnostic procedures It allows sensitive accurate and reproducible quantification of Abeta38 levels
Eurofins Discoverx cAMP Hunter eXpress CXCR2 (IL8RB) CHO-K1 GPCR Assay, 2-Plate Kit. (95-0074E2CP2M)
The cAMP Hunter CXCR2 (IL8RB) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of CXCR2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR2, a naturally Gi/Go coupled GPCR. Upon activation of CXCR2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCKAR Activated GPCR Internalization Assay, 10pl Kit. (93-0650E3CP5L)
The PathHunter CCKAR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCKAR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCKAR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCKAR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CALCR U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0566E3CP5L)
The PathHunter CALCR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. calcitonin) based activation of CALCR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCR and EA-tagged β-Arrestin-2. Activation of CALCR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mFPR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-1034E2MCP2M)
The PathHunter mFPR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. formylpeptides) based activation of mFPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mFPR2 and EA-tagged β-Arrestin-2. Activation of mFPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress SSTR3 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0131E2CP2L)
The cAMP Hunter SSTR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin 28) based activation of SSTR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type SSTR3, a naturally Gi/Go coupled GPCR. Upon activation of SSTR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress BDKRB1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0639E2CP2L)
The PathHunter BDKRB1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. bradykinin) based activation of BDKRB1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BDKRB1 and EA-tagged β-Arrestin-2. Activation of BDKRB1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR1F U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0699E3CP0M)
The PathHunter HTR1F eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1F GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1F and EA-tagged β-Arrestin-2. Activation of HTR1F stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.