General Cell Based Assays
Eurofins Discoverx PathHunter eXpress INSRb Functional Assay, 2-Plate Kit. (93-0466E3CP16M)
The PathHunter eXpress INSRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated INSRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged INSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TrkB Functional Assay, 2-Plate Kit. (93-0463E3CP16M)
The PathHunter eXpress TrkB Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkB dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mRXFP4 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0929E2MCP2L)
The PathHunter mRXFP4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. insulin-like 5, relaxin-3) based activation of mRXFP4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mRXFP4 and EA-tagged β-Arrestin-2. Activation of mRXFP4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc Chicken Focal adhesion kinase
Chicken Focal adhesion kinase 1 (PTK2) ELISA kit from CUSABIO Detection Range 46 88 pg/ml-3000 pg/ml Sensitivity 11 72pg/ml Sample serum plasma tissue homogenates Method
Eurofins Discoverx cAMP Hunter eXpress GCGR CHO-K1 GPCR Assay, 10-Plate Kit. (95-0042E2CP2L)
The cAMP Hunter GCGR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. glucagon) based activation of GCGR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GCGR, a naturally Gs coupled GPCR. Upon activation of GCGR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL17RA/IL17RC Dimerization Assay, 10-plate Kit. (93-0931E1CP7L)
The PathHunter IL17RA/IL17RC Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-17a) induced dimerization of IL17RA/IL17RC receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY1 Activated GPCR Internalization Assay, 2pl Kit. (93-0967E3CP0M)
The PathHunter P2RY1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery RPLEX HUM IGF2R/CIM6PR ASY
The Meso Scale Discovery IGF-2R (CI-MPR) Kit is an electrochemiluminescent immunoassay designed for the quantitative measurement of Insulin-like Growth Factor 2 Receptor (IGF-2R) in human serum plasma and cell culture supernatants This assay employs an MSD GOLD SULFO-TAG label and has a typical lower limit of detection of 0 03 ng/mL The kit includes a 96-well plate coated with an IGF-2R capture antibody a detection antibody conjugated to an MSD SULFO-TAG calibrators positive and negative controls 10X Read Buffer 10X Wash Buffer and Blocker A This assay can be run on the MESO QuickPlex SQ 120 MESO SECTOR S 600 or MESO SECTOR S 6000 instruments
Meso Scale Discovery R-PLEX Human MMP-3 ASY
The R-PLEX Human MMP-3 Assay Kit from Meso Scale Discovery is used to quantitatively measure MMP-3 levels in human serum plasma saliva urine cell culture supernatant and other biological samples It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity a broad dynamic range and requires low sample volume The assay has high specificity for active MMP-3 with no cross-reactivity to pro-MMP-3 The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer diluent and calibrator It can detect MMP-3 levels between 0 03-1000 ng/mL
Abcam MK886, Lipo x ygenase inhibitor, 50MG
MW 494.1 Da, Purity >98%. Potent 5-lipoxygenase activating protein inhibitor (FLAP) (IC₅₀ = 44 nM). Inhibits leukotriene biosynthesis (IC₅₀ = 2.5 nM) in vitro and tumor activity in vivo. Displays PPARα antagonist activity. Orally active in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Molecular Devices Fatty Acid Uptake Assay Kit, Homogeneous, Fluorescence, 96/384-Well
The QBT Fatty Acid Uptake Assay Kit is a homogeneous, fluorescence-based assay for rapid, real-time measurement of fatty acid uptake in live cells. It uses a BODIPY-dodecanoic acid fluorescent analog with quench technology and is configured for high-throughput screening in 96- and 384-well microplates.
- One-step homogeneous fluorescence assay using a BODIPY-dodecanoic acid analog.
- Proprietary quench-based format for real-time fatty acid uptake monitoring.
- Evaluation kit contains 2 vials, sufficient for two microplates.
- Compatible with 96-well and 384-well microplate formats and bottom-read detection.
- Designed for high-throughput screening of fatty acid transporters and uptake inhibitors.
Molecular Devices Neurotransmitter Uptake Assay Kit, Fluorescence, Live-Cell, 96/384, 50-Plate
The Molecular Devices Neurotransmitter Transporter Uptake Assay Kit is a homogeneous, fluorescence-based live-cell assay for measuring uptake activity of serotonin (SERT), norepinephrine (NET), and dopamine (DAT) transporters. The kit uses a fluorescent substrate and masking dye to enable no-wash, mix-and-read endpoint or kinetic measurements on bottom-read microplate readers.
- Homogeneous, no-wash live-cell assay format.
- Fluorescence-based detection suitable for kinetic or endpoint reads.
- Compatible with 96-well and 384-well black clear-bottom plates.
- Bulk kit contains reagents for 50 plates (5 vials of lyophilized dye/masking mix).
- Reconstituted dye solution stable for limited time; store frozen reagents at -20°C.
Molecular Devices CatchPoint cGMP Assay Kit, 384-Well, Clear Bottom
The CatchPoint cGMP Fluorescent Assay Kit Explorer configuration is a high-sensitivity, cell-compatible immunoassay for quantitative measurement of cyclic GMP in high-throughput formats. The kit provides pre-coated 384-well plates and optimized reagents for detecting low cGMP levels with a stable fluorescent readout and flexible timing for automation.
- Pre-coated, pre-blocked 384-well black well, clear bottom plates, two plates per kit
- Includes reagents and buffers for assay setup and detection, including antibody, conjugate, calibrator, and substrate
- Fluorescent Stoplight Red readout with stable signal from 10 minutes up to 24 hours
- Quantitative competitive immunoassay format suitable for cell-based assays and HTS
- Robust format resists interference from colored or fluorescent test compounds
Eurofins Discoverx PathHunter eXpress DARC CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0548E2CP1M)
The PathHunter DARC eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of DARC GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DARC and EA-tagged β-Arrestin-2. Activation of DARC stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.