General Cell Based Assays
Sigma Aldrich Fine Chemicals Biosciences Superoxide Dismutase SOD A
Superoxide dismutase (SOD) is part of a family of metal ion-containing enzymes (SODs) that are found in most organisms from bacteria to mammals. SODs catalyze the dismutation of superoxide (generated by aerobic respiration) to molecular oxygen and peroxide. Superoxide dismutase form the front line of defence against reactive oxygen species (ROS)-mediated injury and harbor anti-inflammatory activities. The Superoxide Dismutase (SOD) Activity Assay Kit provides a simple and sensitive procedure for measuring SOD enzymatic activity in various sample types. SOD activity is determined by measuring the decrease in superoxide anions (generated by the enzyme xanthine oxidase [XO]). Since superoxide anions interact with the provided WST dye yielding color at 450 nm the decrease in the color signal is proportional to SOD inhibition activity. The kit allows the determination of SOD activity in percent inhibition or in units of activity using the provided SOD Enzyme as a standard. The provided
Sigma Aldrich Fine Chemicals Biosciences myo Inositol C d6 98 atom10MG
myo Inositol C d6 98 atom10MG
Cell Biolabs Inc OxiSelect™ Oxygen Radical Antioxidant Capacity (ORAC) Activity Assay Kit, 192 assays
The Oxygen Radical Antioxidant Capacity (ORAC) Assay is a classic tool for measuring the antioxidant capacity of biomolecules from a variety of samples. The ORAC Activity Assay is based on the oxidation of a fluorescent probe by peroxyl radicals by way of a hydrogen atom transfer (HAT) process. Peroxyl radicals are produced by a free radical initiator, which quenches the fluorescent probe over time. Antioxidants present in the assay work to block the peroxyl radical oxidation of the fluorescent probe until the antioxidant activity in the sample is depleted. The remaining peroxyl radicals destroy the fluorescence of the fluorescent probe. Cell Biolabs’ OxiSelect™ ORAC Activity Assay is a fast and reliable kit for the direct measurement of ORAC antioxidant capacity from cell lysate, plasma, serum, tissue homogenates, and food extracts. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, antioxidant standards and unknown samples.
Enzo Life Sciences SOD activity kit (5x96wells)
ELS offers a large collection of superoxide dismutase products for oxidative stress research. This is a versatile assay for the determination of SOD activity. Differentiate between the activity of three SOD isoenzymes: MnSOD, FeSOD, and Cu/ZnSOD. Obtain higher throughput sample analysis with a convenient colorimetric 96 well plate format and fast results in just 10 minutes. Alternative Name: Superoxide dismutase. Applications: Colorimetric detection, Activity assay. Contents: Microtiter plate, Standard, SOD buffer concentrate, Xanthine oxidase, Xanthine solution, Triton x-100, WST-1 reagent
Cell Biolabs Inc CytoSelect™ 24-Well Cell Migration Assay Kit (8 µm, Colorimetric), 5x12 assays
Chemotaxis, a type of cell migration, is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia that are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions. The CytoSelect™ Cell Migration Assay Kit utilizes polycarbonate membrane inserts (8 µm pore size) to assay the migratory properties of cells. The kit contains twelve 8 um polycarbonate membrane inserts in each of five 24-well plates, through which migratory cells are able to pass. Cells are quantified using the provided Cell Stain Solution. The 8 µm pore size is optimal for epithelial and fibroblast cell migration.
Bioassay Systems EnzyChrom™ Catalase Assay Kit. For quantitative determination of catalase activity and evaluation of drug effects on catalase activity.
For quantitative determination of catalase activity and evaluation of drug effects on catalase activity. Key Features: Sensitive and accurate. Use 10 µL sample. Linear detection range 0.2 to 5 U/L catalase activity. Simple and Convenient. The procedure involves adding a Substrate to sample, incubation for 30 min, followed by a Detection Reagent and reading the optical density or fluorescence intensity. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, cell culture etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.2 U/L.
Cell Biolabs Inc CytoSelect™ 24-Well Cell Migration Assay Kit (3 µm, Fluorometric), 12 assays
Chemotaxis, a type of cell migration, is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia that are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions. The CytoSelect™ Cell Migration Assay Kit utilizes polycarbonate membrane inserts (8 µm pore size) to assay the migratory properties of cells. The kit contains twelve 3 um polycarbonate membrane inserts in a 24-well plate, through which migratory cells are able to pass. Cells are quantified using the provided fluorometric dye. The 3 µm pore size is optimal for measuring the migration of leukocytes.
Sigma Aldrich Fine Chemicals Biosciences Dihydrofolate Reductase Assay Kit kit sufficient for 50-100 tests | 1KT
Dihydrofolate Reductase Assay Kit kit sufficient for 50-100 tests | 1KT
Cell Biolabs Inc CELL ADHESION ASSAY- 48 ASSAYS
Full quantitation of cell adhesion with no manual cell countingFluorometric or colorimetric detectionPlates precoated with a uniform substrate layer of a single ECM protein in each row: Collagen I, Collagen IV, Fibrinogen, Fibronectin, and Laminin.Our CytoSelect™ 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with your choice of single ECM protein in each of the first 5 rows, with the last row provided as a negative control. Just follow a simple protocol:Cells are seeded onto the substrate.Adherent cells attach, while non-adherent cells are washed away.Adherent cells are quantified on a standard plate reader or fluorometer.
Indigo Biosciences Human Insulin-Like Growth Factor Receptor 1 (IGF-1R) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 384-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Insulin-Like Growth Factor Receptor 1 reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against Insulin-Like Growth Factor Receptor 1. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request).