General Cell Based Assays
Eurofins Discoverx HitHunter cAMP Assay for Small Molecules, 200/800 dp (96-well/384-well). (90-0075SM2)
This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or Gαi-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Small Molecules, with a protocol written for use in a 384-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 2 plates (384-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Eurofins Discoverx PathHunter eXpress NMBR Total Internalization Assay, 10pl Kit. (93-0939E3CP5L)
The PathHunter NMBR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of NMBR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged NMBR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the NMBR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Ammonia Assay Kit sufficie
Ammonia (NH3) or the ammonium ion (NH4 ) is found in the atmosphere rainwater soil seawater and volcanic areas. It is widely used as fertilizer being an important source of nitrogen for living systems. It also plays a role in animal physiology. Ammonia is produced by amino acid metabolism and is converted to urea in the human liver through the urea cycle. Ammonia acts as a precursor for various other nitrogen compounds. It is a colorless gas with pungent odor and can be easily liquefied. It is also used as a feed supplement in livestock industry.
Abcam Chloride Channel Assay Kit (Colorimetric)
Chloride Channel Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress PDGFRa Functional Assay, 2-Plate Kit. (93-0823E3CP16M)
The PathHunter eXpress PDGFRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated PDGFRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged PDGFRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical AMPK Complexa11nd 1 10ug
Source: Active recombinant human C-terminal His-tagged AMPK complex (α1, β1, and γ1 subunits) expressed in insect cells • Amino acids: 1-559, 1-270, and 1-331 for the α1, β1, and γ1 subunits, respectively • MW: ~146 kDa
Abcam Histone H4 Total Quantification Kit (Colorimetric).
Histone H4 Total Quantification Kit (Colorimetric).
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx cAMP Hunter™ Human PPYR1 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human PPYR1 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PPYR1 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human GHRHR Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human GHRHR Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GHRHR (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress CSF1R/CSF1R Dimerization Assay, 2pl Kit. (93-1061E3CP5M)
The PathHunter CSF1R/CSF1R Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. M-CSF) induced dimerization of CSF1R/CSF1R receptors. This product uses EFC technology. The cell line included overexpresses CSF1R target protein fused to PK alongside CSF1R target protein fused to EA. Upon ligand-induced dimerization of CSF1R/CSF1R, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 10pl. (95-0118Y2-00058)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Abcam Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric)
Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress GPR123 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0417E2ACP1M)
The PathHunter GPR123 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR123 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR123 and EA-tagged β-Arrestin-2. Activation of GPR123 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Molecular Devices FLIPR Calcium 6-QF Assay Kit, Quench-free, 10 plates
The FLIPR Calcium 6-QF Assay Kit (Explorer kit) is a quench-free, mix-and-read fluorescent assay for measuring intracellular calcium flux in high-throughput plate formats. The Calcium 6-QF formulation provides an increased signal window and is suitable for quench-sensitive targets and multiplexed assays.
- Quench-free formulation for quench-sensitive targets and multiplexing.
- Designed for mix-and-read workflows with pre-optimized FLIPR and FlexStation protocols.
- Sufficient reagents for 10 plates in Explorer kit format.
- Each reagent vial for Sample and Explorer kits is sufficient for one 96-, 384-, or 1536-well plate.
- Suitable for endogenous, primary, and stem cell targets with improved signal window and assay robustness.
American Research Products Inc ELF5 Transcription Factor Activity Assay
ELF5 Transcription Factor Activity Assay