General Cell Based Assays
Cayman Chemical SPHK1 asy Buffer1X 14mL
Source Recombinant human C-terminal His-tagged coagulation factor X expressed in insect cells Amino acids 1-488 (full length) MW 52.8 kDa
Cayman Chemical SphngosIn KInase 2 InhIbIto 96
A DAPK1 (IC50 200 nM) and DAPK3 inhibitor selectively binds to DAPK1 DAPK2 and DAPK3 (Kds 300 79 and 280 nM respectively) over Pim-1 Pim-2 and Pim-3 (Kds 1800 6500 and 810 nM respectively) but does inhibit Pim-3 (IC50 200 nM) inhibits phosphorylation of RLC20 in primary human coronary artery smooth muscle cells at 10 UM reduces phenylephrine-induced contractions in isolated mouse aortic rings and increases the time to reach 50 maximal contractions in calyculin A-stimulated isolated rat caudal arterial smooth muscle strips at 10 and 100 UM respectively
Eurofins Discoverx PathHunter C5AR1 Bioassay Kit, 10-Plate. (93-0557Y2-00060)
The PathHunter C5AR1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C5AR1 and EA-tagged β-Arrestin-2. Activation of C5AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Cayman Chemical SphngosIn KInase 1 InhIbIto 96
A DAPK1 (IC50 200 nM) and DAPK3 inhibitor selectively binds to DAPK1 DAPK2 and DAPK3 (Kds 300 79 and 280 nM respectively) over Pim-1 Pim-2 and Pim-3 (Kds 1800 6500 and 810 nM respectively) but does inhibit Pim-3 (IC50 200 nM) inhibits phosphorylation of RLC20 in primary human coronary artery smooth muscle cells at 10 UM reduces phenylephrine-induced contractions in isolated mouse aortic rings and increases the time to reach 50 maximal contractions in calyculin A-stimulated isolated rat caudal arterial smooth muscle strips at 10 and 100 UM respectively
Abcam TMRE Mitochondrial Membrane Potential Assay Kit 200test
TMRE Mitochondrial Membrane Potential Assay Kit
Med Vet International Vitamin B-12, 500Mcg, 100 Tablets, 24/Case
Vitamin B-12, 500Mcg, 100 Tablets, 24/Case Vitamin B12 is an essential vitamin. This means that the body requires vitamin B12 to work properly. Vitamin B12 can be found in foods such as meat, fish, and dairy products. It can also be made in a laboratory. It is often taken in combination with other B vitamins. Products are not always manufacturer specific. Actual product appearance may differ from website image.
Amsbio LLC Heparin Red Kit. For the direc
Heparin Red Kit. For the direct detection of heparin in human plasma
Dojindo Molecular Technologies Inc GSSG/GSH Quantification Kit (200 TESTS) is high reproducibility for detecting GSH, GSSG and total glutathione individually. Easy and simple procedure, takes 1-2 hours.
Glutathione is a tripeptide present in the body, involved in antioxidation, drug metabolism, as well as enzyme substrates of glutathione peroxidase, glutathione S-transferase, and thiol transferase, etc. Glutathione is usually present as reduced form (GSH), but GSH is converted into its oxidized form (GSSG) by stimulation such as oxidative stress. Therefore, the ratio of GSH and GSSG has been noted as an index of oxidative stress. The GSSG/GSH Quantification kit contains the Masking Reagent of GSH. The GSH can be deactivated in the sample by simply adding the Masking Reagent. Therefore, the GSSG is detected by measuring the absorption (λmax = 412 nm) of DTNB (5,5 Edithiobis (2-nitrobenzoic acid) ) using the enzymatic recycling system. Also, GSH can be determined by the quantity by subtracting GSSG from the total amount of glutathione. The kit is limited to quantifying GSH/GSSG concentration from 0.5 μmol/l to 50 μmol/l and GSSG concentration from 0.5 μmol/l to 25 μmol/l.
Invivogen QUANTI-Luc™ Coelenterazine-utilizing Luciferase Detection Medium
QUaNTI-Luc - a coelenterazine-based luminescence assay reagent. Unit Size: 5 pouches (5 x 25ml). QUaNTI-Luc, is an assay reagent containing all the components required to quantitatively measure the activity of Lucia luciferase and other coelenterazine-utilizing luciferases. One pouch prepares 5 x 96 well plates.
Sigma Aldrich Fine Chemicals Biosciences Urea Assay Kit sufficient
Urea is the major end product of nitrogen metabolism in most animals and is produced in a series of reactions in the liver called the urea cycle. In the urea cycle ammonia is converted to urea which is carried by blood to the kidneys for elimination from the body. High levels of urea in the blood may indicate renal failure. Urea levels may also be elevated in response to treatment with certain drugs such as corticosteroids or in response to decreased kidney filtration due to dehydration or congestive heart failure. Decreased blood urea levels can occur in response to liver disease or malnutrition.