General Cell Based Assays
Indigo Biosciences Human Insulin Receptor isoform B (INSRb) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Insulin Receptor, isoform B reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against Insulin Receptor, isoform B. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Indigo Biosciences INDIGO BIOSCIENCES INC Reporter Assay Kit, Human ADRA2A, 1 x 96-well format
This all-inclusive cell-based luciferase reporter assay kit is designed for quantifying functional activity (agonist or antagonist) against the Human Adrenoceptor Alpha 2A (ADRA2A). It utilizes proprietary mammalian cells engineered for constitutive ADRA2A expression. The kit includes ADRA2A Reporter Cells, optimized media, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. Kits must be stored at -80°C.
- Clear, reproducible results
- All-inclusive assay systems
- Exceptional cell viability post-thaw
- Consistent results lot to lot
BPS Bioscience Inc EIF2AK2 Kinase Assay Kit, 96-well plate, 100 reactions, ADP-Glo™ detection, PKR enzyme supplied, kinase substrate, ATP, assay buffer, stability 6 months
EIF2AK2 Kinase Assay Kit, 96-well plate, 100 reactions, ADP-Glo™ detection, PKR enzyme supplied, kinase substrate, ATP, assay buffer, stability 6 months
Lonza Walkersville PyroGene Recombinant Factor C Endpoint Fluorescent Assay
The PyroGene Recombinant Factor C Assay is the evolution of endotoxin detection testing. Lonza scientists have developed a recombinant form of Factor C, the first component in the horseshoe crab clotting cascade activated by endotoxin. Recombinant Factor C (rFC) is activated by endotoxin binding, and the active enzyme then cleaves a synthetic substrate, resulting in the generation of a fluorogenic compound. PyroGene rFC works through a single enzymatic step compared to the multiple step enzymatic process necessary for LAL assays.Benefits:Sensitivity range from 0.005 to 5 EU/mlHigher endotoxin specificity through the elimination of false positive glucan reactionsLess lot-to-lot variabilitySecurity of supplyFDA acknowledged alternative to LALApplications:Water testingTesting plant-based materialContent:2 x 96 tests/vialrFC enzyme solution2 x 6 mL/vial fluorogenic substrate2 x 5 mL/vial rFC assay buffer2 vials endotoxin2 x 30 mL/vial LAL Reagent Water
Biotium XTT Cell Viability Assay Kit (1000 Assays)
The XTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using standard photometric microplate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and to screen cytotoxic agents. XTT is a tetrazolium derivative. Similar to MTT, XTT measures cell viability based on the activity of mitochondrial enzymes in live cells that reduce XTT and are inactivated shortly after cell death. Unlike MTT, XTT is reduced to a highly water-soluble orange-colored product instead of the insoluble formazan from MTT, thus eliminating the solubilization step required for the MTT assay. The amount of water-soluble product generated from XTT reduction is proportional to the number of living cells in the sample and can be photometrically quantified at 475 nm. Continuous color development permits measurements at multiple time points for extended detection range.
Aat Bioquest Screen Quest™ Fluo-8 No Wash Calcium Assay Kit | 10 Plates
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest™ Fluo-8 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Fluo-8 NW which can cross cell membrane. Fluo-8 NW is the brightest calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Fluo-8 AM are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Fluo-8 NW.
Eurofins Discoverx AssayComplete Thawing Reagent T2, 100. (92-4102TR)
AssayComplete Thawing Reagent T2 is an optimized thawing reagent for revival and recovery of Eurofins DiscoverX cell lines. This reagent ensures optimal post-thaw viability, recovery and assay performance upon thawing. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended thawing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Agilent Technologies Seahorse XFe96/XF Pro FluxPak
Seahorse XFe96/XF Pro FluxPak . For use with XFe96 and XF Pro Analyzer. Seahorse XFe96/XF Pro FluxPak includes 18 XFe96/XF Pro sensor cartridges,18 XFe96/XF Pro Cell Culture Microplates, and 1 bottle of Seahorse XF Calibrant Solution 500mL.
Diazyme Laboratories EZ Vitamin D (25-OH) Assay Liquid Kit, 2-reagent, liquid stable, 200 Tests, 3 μL sample volume, 1 x 40 mL R1, 1 x 10 mL R2, 10 min time to results
The Diazyme EZ Vitamin D Assay is designed for the quantitative determination of 25-hydroxyvitamin D (25-OH vitamin D) in human serum and plasma using automated clinical chemistry analyzers. This assay provides a precise and efficient solution for assessing vitamin D sufficiency by detecting both Vitamin D2 and D3 without requiring sample pre-treatment or pre-dilution. It features high throughput capabilities and is traceable to NIST standards. With FDA clearance and CE marking, it ensures compliance and reliability in clinical settings.
- Liquid, two-reagent format
- 200 tests per kit
- Sample volume of 3 μL
- Time to results of approximately 10 min
- Throughput of approximately 300 tests/hour
- Linear range of 7.6 - 147.8 ng/mL
- Precision of <10% coefficient of variation
- Regulatory certified by FDA and CE
- For use with automated clinical chemistry analyzers
Eurofins Discoverx AssayComplete Cell Culture Kit-103, (92-3103G)
AssayComplete Cell Culture Kit-103 is an optimized media kit intended for use in culturing and propagating Eurofins DiscoverX cell lines. This kit contains the base medium, relevant serum, and pen/strep required to produce a full cell culture medium for optimal cell culture results. This product is provided as a 3-component kit which will produce approximately 550 mL of finished media. Please refer to cell line specific datasheet to determine the recommended cell culture kit. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.