General Cell Based Assays
Promega ReliaPrep miRNA Cell and Tissue Miniprep System
ReliaPrep miRNA Cell and Tissue Miniprep System provides complete isolation of total RNA, including microRNA (miRNA) and other small non-coding RNA (sncRNA) subspecies, from a wide variety of cell and tissue types in as quickly as 40 minutes.
Promega Corporation Triglyceride-Glo Assay
Detect Triglyceride from a variety of biological samples works with lysed cells, tissues, medium, and serum samples. No organic extraction, extreme heat, or centrifugation required, with linear triglyceride detection up to 80 μM.
Promega NanoBRET TE Intracellular HDAC Assay, 1,000 assays
NanoBRET Target Engagement Intracellular HDAC Assay measures compound binding at select HDAC target proteins within intact cells. The assay is based on NanoBRET System, an energy transfer technique designed to measure molecular proximity in living cells.
LanthaScreen™ TR-FRET ER beta Competitive Binding Kit
LanthaScreen™ TR-FRET ER beta Competitive Binding Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor (ER) beta.
Thermo Scientific™ LanthaScreen™ TR-FRET ER alpha Coactivator Assay Kit
The LanthaScreen™ TR-FRET ER alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential estrogen receptor (ER) alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment.
Alexa Fluor™ Kinase Tracer 199, 25 μM
Alexa Fluor™ Kinase Tracer 199 is detected by addition of a Eu-labeled anti-tag antibody.
Promega Glucose-Glo Assay, 5ml
A bioluminescent assay for rapid and sensitive measurement of glucose from a variety of sample types. The assay is suitable for detecting altered glucose consumption due to changes in glycolysis or glucose production during gluconeogenesis.
Biotium XTT Cell Viability Assay Kit (1000 Assays)
The XTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using standard photometric microplate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and to screen cytotoxic agents. XTT is a tetrazolium derivative. Similar to MTT, XTT measures cell viability based on the activity of mitochondrial enzymes in live cells that reduce XTT and are inactivated shortly after cell death. Unlike MTT, XTT is reduced to a highly water-soluble orange-colored product instead of the insoluble formazan from MTT, thus eliminating the solubilization step required for the MTT assay. The amount of water-soluble product generated from XTT reduction is proportional to the number of living cells in the sample and can be photometrically quantified at 475 nm. Continuous color development permits measurements at multiple time points for extended detection range.
Cell Viability Incorporated CELL VIABILITY REAGENTS
Cell Viability reagent kit designed for use on automated cell viability analyzers. Four reagent pack with 4 x 200 sample vials.
Aat Bioquest Screen Quest™ Fluo-8 No Wash Calcium Assay Kit | 10 Plates
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest™ Fluo-8 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Fluo-8 NW which can cross cell membrane. Fluo-8 NW is the brightest calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Fluo-8 AM are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Fluo-8 NW.
Eurofins Discoverx PathHunter Detection Kit, 75 (10 plates), 1,000/4,000 dp (96-well/384-well). (93-0001)
The PathHunter Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter cell lines or with custom cell lines made using ProLabel/PK expression vectors. This kit contains lysis buffer and EFC substrate to allow for detection of EFC levels in the assayed cells. This kit contains all components required to assay 10 plates (96/384 well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0001.
Dojindo Molecular Technologies Inc Cellular Senescence Detection Kit (3 plate), detects SA-β-gal with high sensitivity, applicable to both living and fixed cells, for cell sample by fluorescence microscopy or flow cytometry.
SPiDER-βGal is a new reagent to detect β-galactosidase specifically which possesses a high cell-permeability and a high retentivity inside cells. This novel reagent can detect β-galactosidase not only in fixed cells but also in living cells, and also can be applied to quantitative analysis by flow cytometry. Senescence-associated beta-galactosidase (SA-β-gal), which is overexpressed in senescent cells, has been widely used as a marker of cellular senescence. Cellular Senescence Detection Kit – SPiDER-βGal is optimized to detect intracellular SA-β-gal. Modern publications with a high number of citations summarize the various features of cellular senescence on the following page (https://www.dojindo.com/senescent-cell-detection-selection-guide/).