General Cell Based Assays
Cayman Chemical AMPK actIvatr 5mg
An indirect activator of AMPK (EC50 = 11.7 μM); stimulates glucose uptake in L6 myocytes and promotes phosphorylation of ACC, a known target of AMPK; decreases blood glucose concentrations in Zucker diabetic rats, demonstrating its utility in whole animals; inhibits the growth of multiple myeloma cells
Echelon Biosciences Research Labs PI(3,4,5)P3 DiC16 1 mg
PI(3,4,5)P3 diC16 (Phosphatidylinositol 3,4,5-trisphosphate diC16) is a synthetic, purified dipalmitoyl PI(3,4,5)P3.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2. This item is non returnable
Indigo Biosciences Human Adrenoceptor Beta 1 (ADRB1) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Beta 1 reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRB1. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Eurofins Discoverx PathHunter eXpress CSF3R-JAK1 Functional Assay, 2-Plate Kit. (93-0809E3CP16M)
The PathHunter CSF3R-JAK1 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses CSF3R activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged CSF3R cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR35 Activated GPCR Internalization Assay, 10pl Kit. (93-0968E3CP5L)
The PathHunter GPR35 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GPR35 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress FPR3 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0171E2CP2M)
The cAMP Hunter FPR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. humanin) based activation of FPR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FPR3, a naturally Gi/Go coupled GPCR. Upon activation of FPR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR35 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0942E2CP2M)
The PathHunter GPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR35 and EA-tagged β-Arrestin-2. Activation of GPR35 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human MMP-3 ASY
The R-PLEX Human MMP-3 Assay Kit from Meso Scale Discovery is used to quantitatively measure MMP-3 levels in human serum plasma saliva urine cell culture supernatant and other biological samples It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity a broad dynamic range and requires low sample volume The assay has high specificity for active MMP-3 with no cross-reactivity to pro-MMP-3 The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer diluent and calibrator It can detect MMP-3 levels between 0 03-1000 ng/mL
American Research Products Inc Rat regulated on actiVation in
Rat regulated on actiVation in normal T-cell eXpressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit from CUSABIO Detection Range 15 6 pg/mL-1000 pg/mL Sensitivity 7 8 pg/mL Sample serum plasma cell culture supernates tissue homogenates Method Sandwich
Eurofins Discoverx PathHunter eXpress CDC25A Degradation Assay, 10-Plate Kit. (93-0765E3CP0L)
The PathHunter eXpress CDC25A Degradation Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting the cell cycle checkpoint pathway. This kit enables detection of levels of CDC25a, allowing for assessment of CHK-1-activation-induced CDC25a degradation. This product uses EFC technology. The cell line included overexpresses CDC25a target protein fused to ProLabel. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of CDC25a EFC signal is lost. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Perkin Elmer US LLC UATR Sample Position Plate - 7 mm
The PerkinElmer Universal ATR (UATR) Accessory is ideal for the general IR analysis of solids and liquids. The UATR comes in a variety of crystal configurations as well as single/multiple reflections capabilities. It produces high quality spectra through the use of a pressure arm. The pressure arm force indicator ensures sample-to-sample and operator-to-operator reproducibility. The accessory is recognized when it is placed in the spectrometer's sample compartment, the optics are automatically optimized, and the system is ready to be used without additional alignment. This UATR is compatible with the Spectrum One/100/400/65 and Spectrum 3/Frontier spectrometers.
Meso Scale Discovery R-PLEX AB40 4G8 ASY
The Meso Scale Discovery R-PLEX Human Abeta40 4G8 Assay Kit is a multiplex immunoassay that measures Abeta40 levels in human samples such as serum plasma CSF and cell culture supernatants It uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The kit includes a plate coated with a 4G8 antibody to capture Abeta40 along with detection antibodies calibrators controls buffers and other reagents needed to run the assay It allows rapid and accurate quantification of Abeta40 levels which may be useful as a biomarker in Alzheimer s disease research and drug development
Eurofins Discoverx cAMP Hunter eXpress FPRL1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0044E2CP2L)
The cAMP Hunter FPRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of FPRL1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FPRL1, a naturally Gi/Go coupled GPCR. Upon activation of FPRL1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences Cathepsin K drug discovery kit (1x96wells)
The Cathepsin K Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin K. Using a convenient microplate format, cathepsin K activity is monitored fluorimetrically using the substrate provided in the kit. Applications: Fluorescent detection, HTS Activity assay.
Cayman Chemical LPO asy TrIphenylphosphIn 1ea
A menin-MLL1 protein-protein interaction inhibitor binds to menin (Kd 22 nM) and inhibits the interaction between menin and MLL1 in a cell-free assay (IC50 46 nM) reduces cell growth (GI50 3 UM) and induces cell cycle arrest at the G1 phase in MV4-11 cells