General Cell Based Assays
ABclonal Technology TrKC Rabbit mAb
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family This kinase is a membrane-bound receptor that upon neurotrophin binding phosphorylates itself and members of the MAPK pathway Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position Mutations in this gene have been associated with medulloblastomas secretory breast carcinomas and other cancers Several transcript variants encoding different isoforms have been found for this gene
Eurofins Discoverx PathHunter eXpress IFNGR1/IFNGR2 Dimerization Assay, 2pl Kit. (93-1098E3CP5M)
The PathHunter IFNGR1/IFNGR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IFN-g) induced dimerization of IFNGR1/IFNGR2 receptors. This product uses EFC technology. The cell line included overexpresses IFNGR1 target protein fused to PK alongside IFNGR2 target protein fused to EA. Upon ligand-induced dimerization of IFNGR1/IFNGR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL4R/IL2RG Dimerization Assay, 10pl Kit. (93-0988E3CP5L)
The PathHunter IL4R/IL2RG Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-4) induced dimerization of IL4R/IL2RG receptors. This product uses EFC technology. The cell line included overexpresses IL4R target protein fused to PK alongside IL2RG target protein fused to EA. Upon ligand-induced dimerization of IL4R/IL2RG, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ATCC 10 STR Profiling Authentication Services
One complete human cell line authentication service, utilizing Short Tanden Repeat (STR) profiling. Trained experts amplify 17 STR loci plus Amelogenin using Promega’s PowerPlex 18D System. A comprehensive analysis report interprets both karyotypically normal and abnormal cell lines, includes a electropherograms supporting the allele calls at each locus, known reference profiling against the ATCC STR database and a comprehensive interpretation of results. This order contains ten sample kits, mailed upon purchase. Sample kit mailed upon purchase. Download the instructions for sample preparation and submission.
Cell Signaling Technology Phospho-p4442 MAPK Erk12 Thr202Tyr204 197G2 Rabbit mAb InTraSeqtm 3 Conjugate 3023 25 ul
Phospho-p4442 MAPK Erk12 Thr202Tyr204 197G2 Rabbit mAb InTraSeqtm 3 Conjugate 3023 25 ul
ABclonal Technology HRH3 Rabbit pAb
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and can regulate neurotransmitter release. This receptor can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system.
Eurofins Discoverx PathHunter eXpress HTR2C (VGV) U2OS β-Arrestin GPCR Assay, 2pl Kit. (93-0932E3CP19M)
The PathHunter HTR2C(VGV) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C(VGV) GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C(VGV) and EA-tagged β-Arrestin-2. Activation of HTR2C(VGV) stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGFR U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0936E3CP5L)
The PathHunter PTGFR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. cloprostenol) based activation of PTGFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGFR and EA-tagged β-Arrestin-2. Activation of PTGFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Montana Molecular Green fS15-targeted cADDis cAMP Assay - 10mL
Measure real-time cAMP signaling kinetics with this flag-tagged S15 cADDis cAMP sensor. Add fS15-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). Other Montana Molecular tools including BacMam-packaged GPCRs, red fluorescent nucleus-targeted or cytosolic cAMP sensors, or red fluorescent sensors for DAG or PIP2 can easily be co-expressed with this assay.
Eurofins Discoverx PathHunter eXpress TRHR Total Internalization Assay, 2pl Kit. (93-0949E3CP5M)
The PathHunter TRHR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of TRHR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged TRHR and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the TRHR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR2C (VGV) U2OS β-Arrestin GPCR Assay, 10pl Kit. (93-0932E3CP19L)
The PathHunter HTR2C(VGV) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C(VGV) GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C(VGV) and EA-tagged β-Arrestin-2. Activation of HTR2C(VGV) stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical DIrect BIlIrubIn Buffer 8mL
Direct bilirubin buffer has been tested and formulated to work exclusively with Cayman’s Total and Direct Bilirubin Colorimetric Assay Kit (Item No. 701720).