General Cell Based Assays
Innovative Research Inc Hmn uPA Rcmb LMW 100ug
Human Urokinase (uPA) Recombinant Low Molecular Weight 100ug - Human Urokinase (uPA) Recombinant Low Molecular Weight from Innovative Research has been recombinantly produced in insect cells and purified using a combination of conventional and affinity chromatography. This is a frozen liquid buffered in 0.05M Sodium Acetate; 0.1M NaCl; 1mM EDTA; pH 5.0 and a purity of 95%. It is useful as a positive control for ELISA and western blot and and other applications including cell differentiation, cell signaling, clotting assays, fibrinolysis, plasminogen activation, protein-protein interactions, receptor binding, thromboelastography, and thrombolysis. Purified from auto-catalytic digestion of recombinant human urokinase produced in insect cells. Active two-chain low molecular weight human urokinase contains the proteinase domain and is separated from the amino terminal fragment by cleavage at the Lys135-Lys136 bond.
Eurofins Discoverx PathHunter Human PROKR1 Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human PROKR1 Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure PROKR1 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human TNF alpha (IL17RD/TNFR2) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human TNF alpha (IL17RD/TNFR2) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IL17RD/TNFR2 . This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human HRH2 (H2) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human HRH2 (H2) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure HRH2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery R-PLEX Human CD5L ASY
The R-PLEX Human CD5L Assay from Meso Scale Diagnostics is a multiplex immunoassay kit that enables the simultaneous quantitative measurement of CD5L in human serum plasma and cell culture supernatants This assay utilizes MSD s electrochemiluminescent detection technology and is provided in a convenient 96-well plate format The kit includes a CD5L-specific antibody coated plate detection antibody calibrator and assay diluent The assay has a typical sensitivity of 0 3 ng/mL and an upper limit of quantification of 2500 ng/mL for CD5L This product can aid in the quantification of CD5L for life science research applications
Eurofins Discoverx PathHunter eXpress FPR1 Total Internalization Assay, 2pl Kit. (93-0873E3CP0M)
The PathHunter FPR1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of FPR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged FPR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the FPR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GHR-JAK2 Functional Assay, 2-Plate Kit. (93-0829E3CP14M)
The PathHunter GHR-JAK2 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses GHR activation via detection of JAK2 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged GHR cytokine receptor, an untagged CTK JAK2 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Rhod-4 Calcium Assay Kit - No Wash
Rhod-4 Calcium Assay Kit - No Wash
The product is subject to the following: Abcam Restricted Use Statement
Miltenyi Biotec, Inc. h IL 10 Secr. Assay Cell Enrichm.(PE)
Isolation and detection of human IL-10–secreting cells.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Eurofins Discoverx AssayComplete Cell Plating 23 Reagent, 100. (93-0563R23A)
AssayComplete Cell Plating 23 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx cAMP Hunter eXpress SSTR4 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0059E2CP2M)
The cAMP Hunter SSTR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin 28) based activation of SSTR4 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type SSTR4, a naturally Gi/Go coupled GPCR. Upon activation of SSTR4, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery sAPPalpha/sAPPbeta Kit
The SappAlpha/SappBeta Kit from Meso Scale Diagnostics measures alpha and beta secretase activity in biological samples It includes reagents plates substrates detection antibodies buffers and calibrators Using electrochemiluminescent detection it offers sensitive quantification of secretase activity The kit supports high-throughput screening of compounds affecting amyloid precursor protein processing Suitable for cell or tissue extracts it requires small sample volumes and includes reagents for 96 wells processing up to 40 samples in duplicate
Eurofins Discoverx PathHunter eXpress GPR26 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0392E2ACP1M)
The PathHunter GPR26 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR26 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR26 and EA-tagged β-Arrestin-2. Activation of GPR26 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MC5R CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0231E2CP2L)
The PathHunter MC5R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-MSH) based activation of MC5R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC5R and EA-tagged β-Arrestin-2. Activation of MC5R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR2C U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0289E3CP3L)
The PathHunter HTR2C eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C and EA-tagged β-Arrestin-2. Activation of HTR2C stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.