Galactosidase Assay Kits
MilliporeSigma™ Novagen™ BetaRed™ β-Galactosidase Assay Kit
Designed for rapid, sensitive measurement of β-galactosidase activity in cell extracts.
Invitrogen™ β-Gal Assay Kit
Provides reagents required to quickly measure levels of active β-galactosidase expressed in cells transfected with plasmids expressing lacZgene
MilliporeSigma™ Novagen™ BetaFluor™ β-Galactosidase Assay Kit
Designed for rapid, sensitive measurement of β-galactosidase activity in cell extracts.
Medchemexpress LLC β-Galactosidase, E. coli | 9031-11-2 | ≥50U/mg solid | 5 KU
β-Galactosidase (EC 3.2.1.23) is a glycoside hydrolase that breaks down β-glycosidic bonds between galactose and its organic parts. In E. coli, the lacZ gene is the structural gene for β-galactosidase, forming part of the lac operon's induction system. This enzyme can hydrolyze lactose into glucose and galactose, which then enter glycolysis.
- Can catalyze the transgalactosylation of lactose into allolactose
- Allolactose can further be broken into monosaccharides
Abcam Beta Galactosidase Assay Kit
Beta Galactosidase Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam oNPG, beta-galactosidase substrate, 1G
MW 301.25 g/mol. β-galactosidase substrate for colorimetric and EIA applications. Hydrolysed by β-galactosidase to yield galactose and ortho-Nitrophenol, which is yellow in color.
The product is subject to the following: Abcam Restricted Use Statement
AMERICAN RESEARCH PRODUCTS INC ALPHA-GALACTOSIDASE ASSAY KIT
503029843 ALPHA-GALACTOSIDASE ASSAY KIT
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES B-GALACTOSIDASE FROM KLUYV50ML
NC2964735 B-GALACTOSIDASE FROM KLUYV50ML
New England Biolabs, Inc. Endo-β-Galactosidase - 50 ug
Endo-β-Galactosidase is an endoglycosidase that hydrolyzes internal B(1-4)-galactose linkages within a variety of sulfated and nonsulfated poly-N-acetyllactosamine structures, derived from milk, glycoproteins and glycolipids. This enzyme is used for structural and functional analysis of poly-N-acetyllactosamine in glycoconjugates. This endo-beta-galactosidase enzyme from F. keratolyticus exhibits broader substrate specificity and higher catalytic efficiency compared to others from E. freundii and B. fragilis.
- Broader substrate specificity & higher activity compared to others from E. freundii and B. fragilis
- Recombinant enzyme with no detectable endoglycosidase, other exoglycosidase contaminating activities
- Glycerol-free for optimal performance in HPLC and mass spectrometry analysis≥95% purity, as determined by SDS-PAGE and intact ESI-MS
Sigma Aldrich Fine Chemicals Biosciences beta Galactosidase Reporte
-galactosidase (-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of -gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of -gal like O-nitrophenyl--D-galactopyranoside (ONPG). -gal is a cost-effective simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the -galactosidase gene LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.
Medchemexpress LLC Β-Galactosidase, E. coli (EC 3.2.1.23) | 9031-11-2 | 1 KU
Β-Galactosidase (EC 3.2.1.23) is a glycoside hydrolase that hydrolyzes the β-glycosidic bonds formed between galactose and its organic moieties. In E. coli, the lacZ gene is the structural gene of β-galactosidase, which can be used as part of the induction system lac operon. This enzyme can hydrolyze lactose to form glucose and galactose, which then enter glycolysis, or catalyze the transgalactosylation of lactose into allolactose, which can then be cracked into monosaccharides.
- Hydrolyzes β-glycosidic bonds
- Catalyzes transgalactosylation of lactose
- Effective pH range 3.0-8.0
- Optimum pH range 3.5-5.0
- Effective temperature range 55°C-65°C
- Optimum temperature range 55°C-60°C
- White to light yellow solid appearance