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Specific assay kits designed measure the activation levels of small, active GTPases by isolating them via their specific downstream effectors. Kits employ molecular methods including pull-down affinity purification, electrophoresis, and western blotting.
Cell Biolabs’ RhoA Activation Assay Kit provides a simple and fast tool to monitor the activation of RhoA. The kit utilizes easily identifiable Rhotekin RBD Agarose beads (pink in color) to selectively isolate and pull-down the active form of Rho from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rho is detected by western blot analysis using an Anti-RhoA specific antibody. Each kit includes an Immunoblot Positive Control and provides sufficient quantities to perform 20 assays.
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This kit detects RAB10 in human serum plasma tissue homogenates and cell lysates offering a broad detection range of 39 pg/mL to 2500 pg/mL with a sensitivity of 9 77 pg/mL Designed for quantitative analysis it employs a sandwich ELISA methodology ensuring high specificity and accuracy The assay requires a short incubation period of 1-5 hours and uses a sample volume of 50-100 L per test Results are measured at 450 nm providing reliable data for research applications such as studying protein expression regulatory mechanisms or disease associations Its optimized protocol balances efficiency and precision making it suitable for laboratories prioritizing robust performance in RAB10 detection across diverse biological matrices
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