Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ PE Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ PE Positive Selection Kit II is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ PE Positive Selection Kit (Catalog #18557) for even faster cell isolations.
Genscript Corporation Enterokinase, His, Bovine
Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.
Invent Biotechnologies Inc Minute™ Plasma Membrane-Derived Lipid Raft Isolation Kit - Spin Column-Based (20 preps)
Lipid rafts are small membrane domains containing high level of cholesterol and sphingolipids. Lipid rafts have been found in plasma membrane (PM) and internal organellar membranes such as mitochondria-associated membrane (MAMs) and endoplasmic reticulum. Lipid rafts are implicated in numerous cellular processes such as signal transduction, membrane trafficking and protein sorting. Lipid-modified proteins and some transmembrane proteins are concentrated in the rafts while other proteins are excluded. Lipid rafts are also found to be associated with Na+/K+ ATPase on PM. More information: https://inventbiotech.com/products/minute%E2%84%A2-plasma-membrane-derived-lipid-raft-isolation-kit-20-preps?_pos=1&_sid=89f0094ad&_ss=r*For total lipid raft isolation, please refer to MinuteTM Total Lipid Raft Isolation Kit under Cat # LR-039 (Invent Biotechnologies, Inc.).
Invent Biotechnologies Inc Minute™ SDS-Remover (10 ml)
This item has a minimum qty of 3 per supplier requirements.
Sodium dodecyl sulfate is one of the most commonly used detergents for biological research. However, the presence of SDS in the sample could interfere with downstream applications. For instance, SDS must be reduced to below a certain concentration before trypsin digestion of protein can be performed effectively. The presence of SDS in a protein sample could interfere with, and needs to be removed prior to, the analysis. The presence of SDS in protein samples also interferes with antigen-antibody binding and has a negative impact for experiments such as immunoprecipitation and ELISA. This proprietary formulated reagent is designed for fast and easy removal of SDS in solution by precipitation. The efficacy is superior to SDS-removal column and TCA precipitation with minimum protein loss (<20%). Due to the small volume of SDS-Remover used, the final protein concentration of the sample is not significantly impacted.
Invent Biotechnologies Inc Minute™ Protein Solubilization Reagent for MS (2 ml)
This item has a minimum qty of 4 per supplier requirements.
This reagent is formulated for dissolving protein pellets using protein kits from Invent Biotechnologies (Cat# Nos. SM-005, SM-005-P, CP-011, NE-013, YM-017, MM-018 and AF-023) and other companies. Solubilized proteins are denatured due to the presence of SDS and can be used for trypsin digestion and for subsequent mass spectrometry analysis.
Invent Biotechnologies Inc Minute™ Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 Preps)
Preparation of cytosolic and nuclear fractions from cultured cells and tissues is common. Cell fractionation from cultured cells is relatively easy and straightforward. However, a clear separation of cytosolic and nuclear fractions from tissues, especially frozen tissues, is challenging. The cross-contamination from frozen tissues is always an issue because of the altered tissue cellular structure caused by the freeze and thaw cycle, improper homogenization, and the use of an inadequate extraction buffer. More information: https://inventbiotech.com/products/minute-cytosolic-and-nuclear-extraction-kit-for-frozen-fresh-tissues?_pos=1&_sid=2257e16f3&_ss=r
Abcam Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer), 1KIT
ab110171 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells.
The product is subject to the following: Abcam Restricted Use Statement
Biotium GloMelt™ Thermal Shift Protein Stability Kit, with ROX, 2000 assays
GloMelt™ dye can be used to detect protein unfolding or measure thermal stability by performing a thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.
INNOVATIVE BIOCHIPS Single Cell Isolation Chip: 1CellDish-60mm, 64 wells, Pack of 5
1CellDish is a handheld device with microfluidics aliquoting channels designed for single-cell isolation. The operation is by pipetting cell suspension into the center well and distributing cells to the satellite wells to form single-cell aliquots in less than 2 µL volumes. Features: 1) It provides rapid, convenient, and damage-free cell isolation. 2) It simplifies steps for harvesting target cells and genes. 3) It is compatible with all cell sizes smaller than 50 µm at either low or high cell density. 4) It uniformly distributes cell suspension into 64 outlet wells and retrieves single target cells by pipetting. Parameters: 1) Size of device: standard 60 mm Petri Dish format. 2) Number of wells: 64. 3) Well volume: 2.5 µL. 4) Single-cell yield: about 20 cells per device. Summary: 1CellDish isolates cells at a price below $5 per cell. It is used for single-cell lysis, PCR, sequencing, and multiomics.
STEMCELL Technologies EasySep™ Mouse B Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-B cells and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using and EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For isolation of B cells expressing CD11b or CD43, we recommend using the EasySep™ Mouse Pan-B Cell Isolation kit (Catalog #19844 (https://www.stemcell.com/products/easysep-mouse-pan-b-cell-isolation-kit.html)).This product replaces the EasySep™ Mouse B Cell Enrichment Kit (Catalog #19754) for even faster cell isolations.
STEMCELL Technologies EasySep™ Mouse CD11c Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD11c Positive Selection Kit II isolates highly purified CD11c+ cells from splenocytes or other tissues by immunomagnetic positive selection. Desired cells are targeted with antibodies and magnetic particles, and isolated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately ready for downstream applications such as flow cytometry, culture, and cell-based experiments.This product replaces the EasySep™ Mouse CD11c Positive Selection Kit (Catalog #18758) for even faster cell isolations and does not result in the labeling of isolated cells with PE.