Protein Purification Reagents and Kits
Cayman Chemical Soluble Epoxide Hydrolase Inh
Cayman’s fluorescence-based sEH Inhibitor Screening Assay Kit provides a convenient method for screening human sEH inhibitors. The assay utilizes (3-phenyl-oxiranyl)-acetic acid cyano-(6-methoxy-naphthalen-2-yl)-methyl ester (PHOME) as a substrate. When the epoxide moiety of PHOME is hydrolyzed by sEH, an intramolecular cyclization occurs, which results in the release of a cyanohydrin under basic conditions. The cyanohydrin quickly decomposes into cyanide ion and the highly fluorescent 6-methoxy-2-naphthaldehyde,{14265} which can be analyzed using a fluorescence plate reader at excitation and emission wavelengths of 330 and 465 nm, respectively.
Takara Bio TALON® Metal Affinity Resin 10 ml
Immobilized metal affinity chromatography resin for the purification of recombinant his-tagged proteins under native or denaturing conditions. 10 ml
Enzo Life Sciences FLUOR DE LYS HDAC6 fluorometric drug discovery kit (1x96wells)
A FLUOR DE LYS. HDAC6 assay is based on the FLUOR DE LYS Substrate and FLUOR DE LYS Developer combination. The assay procedure has two steps. First, the FLUOR DE LYS Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYSDeveloper produces a fluorophore. Alternative Name: HDAC6 fluorescent assay kit. Applications: Cell-based assays, Activity assay.
Bioworld Protoplast Isolation Kit, 20 Prep(s)
Protoplast Isolation KitProtoplast isolation kit is used to the isolation of protoplast from plant tissue. Contents: all necessary enzymes, buffers and reagents to perform isolation of protoplasts.
Biolegend LEGENDplex™ Mouse L-selectin Capture Bead B5, 13X; Reactivity: Mouse; Apps: Multiplex - Component; Size: 100 tests (270 μL)
LEGENDplex™ Mouse L-selectin Capture Bead B5, 13X; Reactivity: Mouse; Apps: Multiplex - Component; Size: 100 tests (270 μL)
Gold Biotechnology Inc Glutathione Agarose Resin 5 mL
Glutathione has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.
Biolegend MojoSort on Columns - Large Columns Apps Cell Sep - Pos Cell Sep - Neg Size 25 Pack
MojoSort on Columns - Large Columns Apps Cell Sep - Pos Cell Sep - Neg Size 25 Pack
Biotium GloMelt™ Thermal Shift Protein Stability Kit, with ROX, 2000 assays
GloMelt™ dye can be used to detect protein unfolding or measure thermal stability by performing a thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.
STEMCELL Technologies EasySep™ Human CD271 Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Human CD271 Positive Selection Kit II is designed to isolate CD271+ cells from fresh human bone marrow by positive selection. Desired cells are targeted with antibody complexes recognizing CD271 and dextran-coated magnetic particles. The kit also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD271+ antigen is expressed on multiple cell types including mesenchymal stem and progenitor cells (MSCs).This product replaces the EasySep™ Human CD271 Positive Selection Kit (Catalog #18659) for even faster cell isolations.
INNOVATIVE BIOCHIPS Single Cell Isolation Chip: 1CellDish-60mm, 64 wells, Pack of 5
1CellDish is a handheld device with microfluidics aliquoting channels designed for single-cell isolation. The operation is by pipetting cell suspension into the center well and distributing cells to the satellite wells to form single-cell aliquots in less than 2 µL volumes. Features: 1) It provides rapid, convenient, and damage-free cell isolation. 2) It simplifies steps for harvesting target cells and genes. 3) It is compatible with all cell sizes smaller than 50 µm at either low or high cell density. 4) It uniformly distributes cell suspension into 64 outlet wells and retrieves single target cells by pipetting. Parameters: 1) Size of device: standard 60 mm Petri Dish format. 2) Number of wells: 64. 3) Well volume: 2.5 µL. 4) Single-cell yield: about 20 cells per device. Summary: 1CellDish isolates cells at a price below $5 per cell. It is used for single-cell lysis, PCR, sequencing, and multiomics.
STEMCELL Technologies RosetteSep™ Human CD4+ T Cell Enrichment Cocktail, For processing: 200 mL whole blood processed
The RosetteSep™ Human CD4+ T Cell Enrichment Cocktail is designed to isolate CD4+ T cells from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD4+ T cells and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified CD4+ T cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
Active Motif Histone Purification Mini Kit
Active Motif's Histone Purification Mini Kit enables you to purify core histones (H2A, H2B, H3 and H4) as one total population while preserving their post-translational modifications such as acetylation, methylation, and phosphorylation states. This purification method is an improvement over acid precipitation methods and utilizes a convenient spin column and proprietary buffer system to purify the core histones from cells and tissue samples. Histones isolated by this method are suitable substrates for downstream assays and in vitro chromatin assembly.
Zymo Research Corporation Chromatin Wash Buffer II, 30 ml
Highlights: Sold separately or as part of the Zymo-Spin ChIP Kit (D5209, D5210). Storage temperature: room temperature.
Waters Corp Waters BioResolve CX pH Concentrate Kit, pH 5.0, pH 10.2
This kit, designed for use with BioResolve SCX columns, contains 10X buffer concentrates A and B. These concentrates are optimized for creating a pH 5.0 to 10.2 gradient, which is used for mAb charge variant separations. The kit includes one bottle of BioResolve CX pH Concentrate A (pH 5.0) and one bottle of BioResolve CX pH Concentrate B (pH 10.2).
- Mobile phase
- UNSPSC: 41115709
- Application: Protein
- Application kits
- Units per package: 2 pk