Protein Purification Reagents and Kits
BIOHIPPO h/m CD36-sh-RFP/Luc
h/m CD36-sh-RFP/Luc shRNA lentiviral vector expressing RFP/Luc with selection for studies in Human/Mouse Each vial provides a nominal titer of 2x10E6 transducing units TU Validated h/m CD36 shRNA Lentivirus High-titer shRNA lentiviral particles specific for human/mouse CD36 molecule (CD36) The shRNA has been validated to meet or exceed 70% CD36 knockdown efficiency using a specific fluorescence-based method that is more rapid and reliable than qPCR The shRNA lentivirus is ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation and ideal for transducing difficult-to-transfect cells including thawed and/or primary cells Order a shRNA lentivirus set and receive lentivirus produced from mix of 2 independent shRNAs validated to knockdown the target gene ( 70%) plus control lentivirus produced from mix of 2 scrambled shRNA constructs
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit, For processing: 60 mL whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit is designed to isolate CD8+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD8 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD8 antigen is expressed on cytotoxic T cells and weakly on a subset of NK cells.This product replaces the EasySep™ Human Whole Blood CD8 Positive Selection Kit (Catalog #18083) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications. Effector memory CD4+ T cells can be isolated from the CD62L- fraction.
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit, For processing: 60 mL whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit is designed to isolate highly purified myeloid (including CD14+ and CD66b+) cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 antigen is expressed on myeloid progenitor cells, granulocytes and monocytes.This product replaces the EasySep™ Human Whole Blood CD33 Positive Selection Kit (Catalog #18287) and EasySep™ HLA Whole Blood CD33 Positive Selection Kit (Catalog #18287HLA) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human ILC2 Isolation Kit, For processing: 2 x 10^9 cells
The EasySep™ Human ILC2 Isolation Kit is designed to isolate Group 2 Innate Lymphoid Cells (ILC2s) from leukapheresis products by immunomagnetic positive selection without the use of a flow sorter.First, CRTH2+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CRTH2+ cells, and unwanted non-ILC2s are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified ILC2s that are immediately ready for downstream applications.Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
Isolate highly purified CD33+ and CD66b+ myeloid cells, including myeloid progenitor cells, granulocytes, and monocytes, from fresh whole blood prior to chimerism testing. Desired cells are targeted with tetrameric antibody complexes recognizing CD33, CD66b, and dextran-coated magnetic particles, and separated without the use of columns through immunomagnetic positive selection using an EasySep™ magnet. Cells of interest remain in the tube while unwanted cells are poured off. This product can be used with RoboSep™ to automate immunomagnetic cell isolation. Visit www.EasySep.com for more information.
STEMCELL Technologies EasySep™ Human CD56 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD56 Positive Selection Kit II is designed to isolate CD56+ cells from fresh or previously frozen peripheral blood mononuclear cells and human skeletal muscle (myoblasts and fibroblasts) cultures by positive selection. Desired cells are targeted with antibody complexes recognizing CD56 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD56 antigen is expressed on NK cells, NKT cells and human myoblasts.This product replaces the EasySep™ Human CD56 Positive Selection Kit (Catalog #18055) for even faster cell isolations.
BIOHIPPO AAV-EF1a-tettoxlc-P2A-EGFP-WPRE-hGH-polyA
Recombinant adeno associated virus expressing Tetanus toxin light chain fused to EGFP under the EF1a promoter with WPRE and hGH polyA elements. The Tet responsive configuration enables doxycycline controlled expression, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
Biotium Primary Antibody CD8a SK1 CF503R 1/EA
CD8a (clone SK1) is a mouse monoclonal antibody that recognizes the human protein CD8 CD8a is a common marker of cytotoxic (Killer) T cells and is a T cell coreceptor This CF503R antibody conjugate has been validated in flow cytometry
BIOHIPPO h/m P53-sh-RFP/Luc
h/m P53-sh-RFP/Luc shRNA lentiviral vector expressing RFP/Luc with selection for studies in Human/Mouse Each vial provides a nominal titer of 2x10E6 transducing units TU Validated human P53 shRNA Lentivirus High-titer shRNA lentiviral particles specific for human tumor suppressor protein p53 (P53/TP53) The shRNA has been validated to meet or exceed 70% P53 knockdown efficiency using a specific fluorescence-based method that is more rapid and reliable than qPCR The shRNA lentivirus is ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation and ideal for transducing difficult-to-transfect cells including thawed and/or primary cells Order a shRNA lentivirus set and receive lentivirus produced from mix of 2 independent shRNAs validated to knockdown the target gene ( 70%) plus control lentivirus produced from mix of 2 scrambled shRNA constructs
Genscript Corporation CD4/LEU3 His Mouse
CD4 also known as L3T4 T4 and W3/25 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes It is a standard phenotype marker for the identification of T cell populations Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses In T-cells functions primarily as a coreceptor for MHC class II molecule peptide complex