Protein Purification Reagents and Kits
Promega NanoBRET™ PPI MCS Starter System
Protein:Protein Interaction (PPI) Assays use NanoLuc Luciferase and HaloTag™ protein fused to target proteins of interest to enable sensitive, reproducible detection of protein interactions in the natural cellular environment.
G-Biosciences DetergentOUT™ Tween™, Gravity Flow Column
Pre-packed, spin and gravity flow column format, detergent removal resin columns for polysorbate or Tween™ detergents or surfactants.
G-Biosciences DetergentOUT™ Tween™, Maxi
DetergentOUT™ Tween™, Maxi (10 mL) is used for fast and efficient removal of polysorbate or Tween™ detergents.
Anti-DYKDDDDK Affinity Resin, G-Biosciences
Anti‐DYKDDDDK Affinity Resin is suitable for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems. DYKDDDDK, commonly known as FLAG™ sequence (FLAG™ is a registered trademark of Sigma).
STEMCELL Technologies EasySep™ Dead Cell Removal (Annexin V) Kit, For processing: 1 x 10^9 cells
The EasySep™ Dead Cell Removal (Annexin V) kit is designed to deplete apoptotic (Annexin V+) cells from cell culture or tissue preparations by immunomagnetic negative selection. Unwanted cells are labeled with Annexin V, antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are simply poured off into a new tube. Annexin V binds phosphatidylserine on the outer leaflet of the cell membrane during apoptosis.
Biolegend MojoSort™ Mouse CD3 T Cell Isolation Kit
MojoSort™ Mouse CD3 T Cell Isolation Kit Reactivity: Mouse; Apps: Cell Sep - Neg; Size: 100 tests
STEMCELL Technologies EasySep™ Human Cord Blood CD34 Positive Selection Kit II, For processing: 1000 mL cord blood processed
Complete kit for CD34+ cells from cord blood
STEMCELL Technologies RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36, For processing: 40 mL whole blood processed
The RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36 is designed to enrich circulating epithelial tumor cells from fresh whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD16, CD19, CD36, CD38, CD45, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a density gradient medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified tumor cells are present as a highly enriched population at the interface between the plasma and the density gradient medium.This cocktail is compatible with small-cell carcinoma samples, including lung cancer samples. If you are working with breast cancer samples, use the RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD56 (Catalog #15137).
STEMCELL Technologies EasySep™ Human CD4 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD4 Positive Selection Kit II is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing CD4 and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human CD4 Positive Selection Kit (Catalog #18052) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human CD34 Positive Selection Kit II, For processing: 5 x 10^9 cells
The EasySep™ Human CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh or previously frozen bone marrow or mobilized peripheral blood mononuclear cells, or from previously frozen cord blood mononuclear cells, or from hESC/hiPSC-derived hematopoietic cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.This product can be used in place of the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056) for even faster cell isolations.
STEMCELL Technologies RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail, For processing: 40 mL bone marrow processed
The RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail is designed to isolate mesenchymal stem cells from fresh bone marrow by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD3, CD14, CD19, CD38, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified mesenchymal stem cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
ILEX LIFE SCIENCES LLC Biocolor Blyscan Glycosaminoglycan (sGAG) Assay, Standard Size Kit (110 assays)
The Biocolor Blyscan™ Glycosaminoglycan Assay is a quantitative colorimetric dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans (sGAG). The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples. The dye label used in the assay is 1,9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains. Applications: Cell culture derived material, soluble sGAGs from fluids (ex. synovial fluid, urine), fibrous and hyaline cartilages, tissues (ex. arteries, heart, lung, skin and other material containing extracellular matrix, tumors). Soluble sGAGs may be assayed directly, solid materials will require a preliminary papain extraction. Sensitivity: 0.5 µg. Assay run time: 1 hour.
STEMCELL Technologies EasySep™ Human CD4+ T Cell Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human CD4+ T Cell Enrichment Kit is designed to isolate CD4+ T cells from fresh or previously frozen peripheral mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD4+ T cells and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For even faster cell isolations, we recommend the new EasySep™ Human CD4+ T Cell Isolation Kit (17952) (http://www.stemcell.com/en/Products/All-Products/EasySep-Human-CD4-T-Cell-Isolation-Kit.aspx) which isolates cells in just 8 minutes.