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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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556 – 570 of 5,115 results
556

Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns

Designed for method optimization and parameter screening

Buffer 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
Column Type Ion Exchange Column
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
For Use With (Application) Optimization and parameter screening, ion exchange chromato graphy
Max. Pressure 1.5 bar
Particle Size 45 to 165μm
Flow Rate <450 cm/hr.
Support Type 6% Cross-linked Agarose
Height (English) 3.93 in.
557

Cytiva HiScreen Prepacked HIC Column, Capto Butyl

Intended for initial and intermediate step purifications that require a medium with low to medium hydrophobicity.

558

Cytiva HiTrap™ Protein L Prepacked Columns

For capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs

Particle Size 85μm
Ligand Type Recombinant Protein L (E. coli), mammalian free
Flow Rate <500 cm/hr.
Height (Metric) 20mm
Content And Storage 2° to 8°C
pH Range 2 to 10
Product Type HiTrap Protein L column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Affinity chromato graphy medium (Resin) for capture of Antibodies and Antibody fragments such as Fab fragments, scFv and Dabs
Support Type Highly Cross-linked Agarose
Max. Pressure 5 bar
Height (English) 0.78 in.
559

Cytiva HiTrap Capto S ImpAct Prepacked Columns

HiTrap Capto S ImpAct columns are prepacked with Capto S ImpAct cation exchange chromatography medium for screening and small-scale protein purifications using ion exchange chromatography (IEX).

Particle Size 50μm
pH Range 4 to 12
Product Type HiTrap Capto S ImpAct Prepacked Column
Column Type Ion Exchange Column
Material High-flow agarose
560

Cytiva HiScale™ Chromatography Columns
GSA_VA

Withstand pressures of up to 20 bar (290 psi) and high flow rates. Cytiva Life Sciences™ HiScale™ Chromatography Columns are designed to be compatible with modern BioProcess chromatography media such as MabSelect™ and Capto™.

Includes Column tube, outer protection tube, two adapters, PEEK tubing, and unions at both ends for easy connection to valves, pumps, and monitors
Product Type HiScale Chromatography Column
Column Type Empty HPLC Column
Material Borosilicate glass
For Use With (Application) Process development and preparative chromatography
Support Type Polypropylene (reinforced glassfibre)/Polyamide Net
Max. Pressure 20 bar
561

Thermo Scientific™ POROS™ CaptureSelect™ LC Lambda Affinity Column, 4.6 x 50 mm

Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.

562

Thermo Scientific™ POROS™ CaptureSelect™ GCSF Affinity Column, 4.6 x 100 mm

Thermo Scientific™ POROS™ CaptureSelect™ GCSF HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands.

563

Thermo Scientific™ POROS™ CaptureSelect™ IgA Affinity Column, 4.6 x 50 mm

Thermo Scientific™ POROS™ CaptureSelect™ IgA HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands.

564

Cytiva HiTrap Capto MMC Columns

HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

Particle Size 75μm
Buffer Aqueous Buffer
Height (Metric) 25mm
Content And Storage 4° to 30°C
pH Range 2 to 12
Product Type HiTrap Capto MMC column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Bind Proteins at the conductivity of the feed material and to solve specific purification challenges
Support Type Highly Cross-linked Agarose
Height (English) 0.98 in.
565

Cytiva HiTrap™ Chelating HP Columns
GSA_VA

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

Particle Size 34μm
Buffer 6M Guanidine Hydrochloride, 8M Urea
Ligand Type Iminodiacetic Acid
Height (Metric) 25mm
Content And Storage 4° to 30°C
pH Range 3 to 13
Product Type HiTrap chelating HP column
Column Type Affinity Column
For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 3 bar
Height (English) 0.98 in.
566

Cytiva DEAE-Sepharose™ Media

Developed for process-scale chromatography

567

Cytiva Chelating Sepharose™ Fast Flow Medium

Use for purifying native and recombinant proteins and peptides that have an affinity for metal ions. Chelating Sepharose™ Fast Flow Medium provides robust performance and regulatory support.

568

Cytiva GSTrap™ FF Columns: 7 x 25mm

Purifies GST-tagged proteins in one step

Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.27 in.
Bed Volume 1 mL
Description GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
pH Range 3 to 12
Diameter (Metric) Inner 7 mm
Material Polypropylene
For Use With (Application) For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract
Particle Size 90 μm
Ligand Type Glutathione and 10 carbon linker arm
Flow Rate <4 mL/min.
Type Pre-packed Column
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Support Type 4% Highly Cross-linked Agarose
569

Cytiva GSTrap™ 4B Prepacked Columns: 7 x 25mm

Use for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ 4B Prepacked Columns: 7 x 25mm are prepacked with Glutathione Sepharose™ 4B

Ligand Type Glutathione
Flow Rate <4 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.27 in.
Bed Volume 1 mL
Description GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 4 to 13
Diameter (Metric) Inner 7 mm
Material Polypropylene
For Use With (Application) GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.
Support Type 4% Highly Cross-linked Agarose
570

Cytiva PD MidiTrap™ G-10

Cleanup biological samples, such as peptides, small proteins, and oligosaccharides. Cytiva Life Sciences™ PD MidiTrap™ G-10 offers fast desalting and efficient removal of contaminants such as salts, dyes, and radioactive labels.