Protein Purification Reagents and Kits
STEMCELL Technologies RosetteSep™ Human Multiple Myeloma Cell Enrichment Cocktail, For processing: 200 mL bone marrow processed
The RosetteSep™ Human Multiple Myeloma Cell Enrichment Cocktail is designed to enrich multiple myeloma cells (B cell and plasma cells) from fresh bone marrow aspirates by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD14, CD33, CD41, CD45RA, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified multiple myeloma cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies EasySep™ Human B Cell Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human B Cell Enrichment Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-B cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For even faster cell isolations, we recommend the new EasySep™ Human B Cell Isolation Kit (17954) (/products/easysep-human-b-cell-isolation-kit.html), which isolates cells in just 9 minutes.
STEMCELL Technologies RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail, For processing: 200 mL bone marrow processed
The RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail is designed to isolate mesenchymal stem cells from fresh bone marrow by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD3, CD14, CD19, CD38, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified mesenchymal stem cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit is designed to isolate CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors but is lost upon maturation to plasma cells. It is also expressed on follicular dendritic cells.This product replaces the EasySep™ Human Whole Blood CD19 Positive Selection Kit (Catalog #18084) for even faster cell isolations.
ILEX LIFE SCIENCES LLC Biocolor Blyscan Glycosaminoglycan (sGAG) Assay, Standard Size Kit (110 assays)
The Biocolor Blyscan™ Glycosaminoglycan Assay is a quantitative colorimetric dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans (sGAG). The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples. The dye label used in the assay is 1,9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains. Applications: Cell culture derived material, soluble sGAGs from fluids (ex. synovial fluid, urine), fibrous and hyaline cartilages, tissues (ex. arteries, heart, lung, skin and other material containing extracellular matrix, tumors). Soluble sGAGs may be assayed directly, solid materials will require a preliminary papain extraction. Sensitivity: 0.5 µg. Assay run time: 1 hour.
STEMCELL Technologies EasySep™ Human CD4+ T Cell Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human CD4+ T Cell Enrichment Kit is designed to isolate CD4+ T cells from fresh or previously frozen peripheral mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD4+ T cells and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For even faster cell isolations, we recommend the new EasySep™ Human CD4+ T Cell Isolation Kit (17952) (http://www.stemcell.com/en/Products/All-Products/EasySep-Human-CD4-T-Cell-Isolation-Kit.aspx) which isolates cells in just 8 minutes.
CUBE BIOTECH PureCube Ni-NTA Agarose; 1000 ml
1 L Ni-NTA MagBeads for His-tag-purification, 25% slurry
STEMCELL Technologies RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail, For processing: 40 mL bone marrow processed
The RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail is designed to isolate mesenchymal stem cells from fresh bone marrow by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD3, CD14, CD19, CD38, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified mesenchymal stem cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies EasySep™ Human CD34 Positive Selection Kit II, For processing: 5 x 10^9 cells
The EasySep™ Human CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh or previously frozen bone marrow or mobilized peripheral blood mononuclear cells, or from previously frozen cord blood mononuclear cells, or from hESC/hiPSC-derived hematopoietic cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.This product can be used in place of the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056) for even faster cell isolations.
STEMCELL Technologies RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36, For processing: 40 mL whole blood processed
The RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36 is designed to enrich circulating epithelial tumor cells from fresh whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD16, CD19, CD36, CD38, CD45, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a density gradient medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified tumor cells are present as a highly enriched population at the interface between the plasma and the density gradient medium.This cocktail is compatible with small-cell carcinoma samples, including lung cancer samples. If you are working with breast cancer samples, use the RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD56 (Catalog #15137).
STEMCELL Technologies EasySep™ Human Cord Blood CD34 Positive Selection Kit II, For processing: 1000 mL cord blood processed
Complete kit for CD34+ cells from cord blood
STEMCELL Technologies EasySep™ Human CD4 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD4 Positive Selection Kit II is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing CD4 and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human CD4 Positive Selection Kit (Catalog #18052) for even faster cell isolations.
Novus Biologicals Maurice cIEF pI Marker - 6.14, Lyophilized, 210 uL
Maurice C. reagents and consumables are designed for use on with Maurice and Maurice C. systems, and must be used in conjunction with Compass for iCE version 1.0 software.
Cytiva Biacore maintenance kit (type 3)
Kit for conducting instrument maintenance of Biacore 8K+ and Biacore 8K SPR systems, containing BIAtest, BIAdesorb and BIAnormalizing solutions.