Protein Purification Reagents and Kits
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Filtered Search Results
Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
| Particle Size | 90μm |
|---|---|
| Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
| Flow Rate | 150 to 600cm/hr. |
| Content And Storage | 4° to 30°C |
| pH Range | 2 to 12 |
| Product Type | Ni Sepharose excel chromato graphy media |
| dimMatrix | Ni-Sepharose |
| Support Type | 6% Highly Cross-linked Agarose |
Thermo Scientific™ Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X) for Pierce™ Primary Cell Isolation Kits
Easily isolate and culture highly viable functional primary cardiomyocytes
Thermo Scientific™ Pierce™ ChIP-Grade Protein A/G Plus Agarose
Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.
| Stationary Phase | Protein A/G |
|---|---|
| Type | Agarose |
| Target | IgG Antibody |
| Content And Storage | Upon receipt store at 4°C |
| Format | Bottle |
| Form | Liquid Suspension |
| Column Type | Affinity, Agarose Resin |
| Product Line | Pierce |
Thermo Scientific™ Pierce™ Chromatin Prep Module
Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.
Thermo Scientific™ ActivX™ TAMRA-FP Serine Hydrolase Probe
ActivX™ probes have been designed to selectively label and enrich serine hydrolases through binding of their respective active sites with tagged fluorophosphonate to enable assessment of enzyme activity.
Thermo Scientific™ Pierce™ Glycoprotein Isolation Kit, WGA
Purify glycoproteins from serum and cell lysates using affinity resins or kits with concanavilin A (ConA) or wheat germ agglutinin (WGA) lectins.
Thermo Scientific™ ActivX™ Azido-FP Serine Hydrolase Probe
ActivX™ probes have been designed to selectively label and enrich serine hydrolases through tagged fluorophosphonate to assess enzyme activity. ActivX Serine Hydrolase Probes covalently label serine hydrolase using fluorophosphonate conjugated with either desthiobiotin or azide.
Thermo Scientific™ SMALP 300
Thermo Scientific SMALP 200 and SMALP 300 are styrene maleic acid (SMA) copolymers designed for the solubilization and stabilization of membrane proteins. These innovative products offer a streamlined approach to membrane protein isolation by combining solubilization and stabilization into a single step.
| Content And Storage | Store at 4°C |
|---|---|
| Sensitivity | Sensitive to light |
| Form | Liquid |
| Product Type | Polymer |
| Reagent Type | Extraction and stabilization |
| pH | 8 |
| Purification Target | Membrane proteins |
| For Use With (Application) | Structure/Function |
| Formula Weight | 10,000 Da |
| Product Line | Thermo Scientific |
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
| Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C |
| Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Material | Polypropylene |
| Support Type | 6% Highly Cross-linked Agarose |
Thermo Scientific™ Lauryldimethylamine-N-oxide (LDAO), LC/MS grade
Lauryldimethylamine-N-oxide (LDAO) is a zwitterionic surfactant that possesses unique properties for solubilization and stabilization of biomolecules, particularly membrane proteins.
| CAS | 1643-20-5 |
|---|---|
| Form | Powder |
| Product Type | Lauryldimethylamine-N-oxide |
| Molecular Weight (g/mol) | 229.4 |
| Color | White |
| Purity | ≥99% |
| For Use With (Application) | LC-MS |
| Molecular Formula | C14H31NO |
Thermo Scientific™ Tetraethylene Glycol Monooctyl Ether (C8E4), LC/MS grade
Tetraethylene Glycol Monooctyl Ether (C8E4) is a non-ionic surfactant that possesses unique properties making it suitable for solubilization of sensitive biomolecules without significant denaturation or disruption of their structure and function.
Novex™ Protein A
Protein A
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ SMALP 200
Thermo Scientific SMALP 200 and SMALP 300 are styrene maleic acid (SMA) copolymers designed for the solubilization and stabilization of membrane proteins. These innovative products offer a streamlined approach to membrane protein isolation by combining solubilization and stabilization into a single step.
| Content And Storage | Store at 4°C |
|---|---|
| Sensitivity | Sensitive to light |
| Form | Liquid |
| Product Type | Polymer |
| Reagent Type | Extraction and stabilization |
| pH | 7.5 |
| Purification Target | Membrane proteins |
| For Use With (Application) | Structure/Function |
| Formula Weight | 6,500 Da |
| Product Line | Thermo Scientific |
Thermo Scientific™ HCT 116 (+hIGF-1) Control Lysate (1mg/mL)
Thermo Scientific™ HCT 116 (+hIGF-1) Control Lysate is a stimulated cell lysate that has been validated for the expression levels and phosphorylation of key targets in the RAS signaling pathway.
Thermo Scientific™ Pierce™ GST Spin Purification Kit, 3 mL
Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.