Restriction Enzymes
Promega Restriction Enzymes - Rsa I (C)
Capable of digesting DNA in 15 minutes or less. Available at high concentration, containing 5,000 units of RsaI at a concentration of 40-80u/μL. (GT/AC CA\TG)
Promega Restriction Enzymes - Sal I Blue/White Cloning and Genome Qualified (D)
Capable of digesting DNA in 15 minutes or less. Genome qualified to ensure optimal performance in genomic analysis applications. (G/TCGA C C AGCT\G)
Promega Restriction Enzymes - Dpn I (B)
Capable of digesting DNA in 15 minutes or less. (G(me)A/TC CT\(me)AG)
Promega Rapid Digestion Kit---Trypsin, 100microg
The Rapid Trypsin and Rapid Trypsin/Lys-C Digestion Kits are designed to shorten protein digestion times to 60 minutes versus the typical 4-18 hours.
Promega Restriction Enzymes - Xho I Blue/White Cloning and Genome Qualified (D)
Capable of digesting DNA in 15 minutes or less. Genome qualified to ensure optimal performance in genomic analysis applications. (C/TCGA G G AGCT\C)
Promega Restriction Enzymes - Hind III Blue/White Cloning Qualified (E)
Capable of digesting DNA in 15 minutes or less. Star activity may be observed in the presence of Mn2+. (A/AGCT T T TCGA\A)
Promega Restriction Enzymes - Pst I Blue/White Cloning Qualified (H)
Capable of digesting DNA in 15 minutes or less. Blue/white cloning qualified, provides a higher level of quality control for enzymes used in cloning applications. (C TGCA/G G\ACGT C)
Promega Restriction Enzymes - Sma I Blue/White Cloning and Genome Qualified (J)
XmaI is a neoschizomer of SmaI. Available at high concentration, containing 5,000 units of SmaI at a concentration of 40-80u/μL. (CCC/GGG GGG\CCC)
Promega Rapid Digestion Kit---Trypsin/Lys-C, 100microg
The Rapid Trypsin and Rapid Trypsin/Lys-C Digestion Kits are designed to shorten protein digestion times to 60 minutes versus the typical 4-18 hours.
Promega Restriction Enzymes - Nde I (D)
Active and capable of digesting DNA in 15 minutes or less, directly in GoTaq™ Green Master Mix. (CA/TA TG GT AT\AC)
Promega Restriction Enzymes - BamH I Blue/White Cloning Qualified (E)
Capable of digesting DNA in 15 minutes or less. Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications. (G/GATC C C CTAG\G)
Dryzyme™ Restriction Enzymes
Three most frequently used restriction enzymes for biotechnology.
New England Biolabs, Inc. SpeI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence A^CTAGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. PstI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence C_TGCA^G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BamHI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^GATC_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.