Restriction Enzymes
New England Biolabs, Inc. HpyCH4V – 100 units
A restriction endonuclease that recognizes the sequence TG_^CA.
New England Biolabs, Inc. AsiSI – 500 units
A restriction endonuclease that recognizes the sequence GCG_AT^CGC.
New England Biolabs, Inc. BfuAI – 250 units
A restriction endonuclease that recognizes the sequence ACCTGCNNNN^NNNN_.
New England Biolabs, Inc. BtgZI – 500 units
A restriction endonuclease that recognizes the sequence GCGATGNNNNNNNNNN^NNNN_.
New England Biolabs, Inc. BtgZI – 100 units
A restriction endonuclease that recognizes the sequence GCGATGNNNNNNNNNN^NNNN_.
New England Biolabs, Inc. CspCI – 500 units
A restriction endonuclease that recognizes the sequence _NN^NNNNNNNNNNNCAANNNNNGTGGNNNNNNNNNN_NN^.
New England Biolabs, Inc. Nb.BsrDI – 5000 units
A restriction endonuclease that recognizes the sequence GCAATG_NN.
New England Biolabs, Inc. FatI – 250 units
A restriction endonuclease that recognizes the sequence ^CATG_.
New England Biolabs, Inc. PspXI – 1000 units
A restriction endonuclease that recognizes the sequence VC^TCGA_GB.
New England Biolabs, Inc. Eco53kI – 1000 units
A restriction endonuclease that recognizes the sequence GAG_^CTC.
New England Biolabs, Inc. NotI – 500 units
A restriction endonuclease that recognizes the sequence GC^GGCC_GC.
New England Biolabs, Inc. SbfI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence CC_TGCA^GG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.