Restriction Enzymes
New England Biolabs, Inc. PvuII-HF – 25000 units
A high fidelity restriction endonuclease that recognizes the sequence CAG^CTG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. MspJI – 1000 units
A restriction endonuclease that recognizes the sequence CNNRNNNNNNNNN^NNNN_.
Cayman Chemical Myrca rubra bark Myrctrn 10mg
A flavonoid with diverse biological activities; active against B. subtilis, E. coli, K. pneumoniae, S. aureus, and C. albicans (MIC = 0.25 mg/ml for all); inhibits AChE and α-glucosidase in vitro (IC50s = 65 and 79 μg/ml, respectively); inhibits LPS-induced production of TNF-α and enhances LPS-induced RANTES production in RAW 264.7 cells at 0.2 and 1 mM
New England Biolabs, Inc. Haelll Methyltransferase – 500 units
HaeIII Methyltransferase modifies the internal cytosine residue (C5) of the sequence GGCC. This enzyme comes from an E. coli strain that carries the cloned HaeIII modification gene from Haemophilus aegyptius (ATCC 11116).
ABclonal Technology BbsI
5...G A A G A C (N)2 ...3 3...C T T C T G (N)6 ...5 Isoschizomers: BpiI, BstV2I
Apexbio Technology LLC Dpn I(Synonyms: DpnI, DpnI Restriction Enzyme, Dam methylation-sensitive restriction enzyme DpnI), 5000U.
Dpn I is a restriction endonuclease sensitive to Dam methylation specifically recognizing and cleaving the adenine N6-methylated sequence GA/TC within DNA Cleavage is dependent on adenine methylation status mammalian CpG methylation overlapping Dpn I sites blocks cleavage and methylation by Dcm is not recognized by this enzyme It is routinely used for the rapid digestion of plasmid DNA PCR fragments and genomic DNA and finds broad utility in genetic typing molecular cloning Southern blotting SNP genotyping and restriction fragment length polymorphism (RFLP) analyses
New England Biolabs, Inc. BsmAI – 1000 units
A restriction endonuclease that recognizes the sequence GTCTCN^NNNN_.
New England Biolabs, Inc. DpnII – 5000 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. HinfI – 25000 units
A restriction endonuclease that recognizes the sequence G^ANT_C.
New England Biolabs, Inc. MboI – 2500 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. BsaJI – 1000 units
A restriction endonuclease that recognizes the sequence C^CNNG_G.