Substrates
Advanced Biomatrix CytoSoft 6-well Plate, Elastic Modulus 32 kPa
This CytoSoft product, Catalog Number 5144, has an elastic modulus of approximately 32 kPa in a standard flat-bottom 6-well plate. The thickness of the silicone gel is uniform with a ~0.5 mm thick layer of silicone in each well that is fully compatible with mammalian cell cultures. The plates are packaged individually, sterilized using irradiation and provided with 5 plates per package. The rigidity of the substrate to which cells adhere can have a profound effect on cell morphology and gene expression.The silicone substrates of CytoSoft products are optically clear and have a low auto-florescence. The layer of silicone in each well is firmly bonded to the bottom of the well. Unlike hydrogels (such as polyacrylamide gels), silicone gels are not susceptible to hydrolysis, do not dry nor swell, are resilient and resistant to tearing or cracking, and their elastic moduli (rigidities) remain nearly unchanged during extended storage periods.
AnaSpec SUC-LLVY-AMC, FLUOROGENIC 5MG
Suc-LLVY-AMC, fluorogenic substrate[Suc-LLVY-AMC] ; MW 763.9; (5 mg) This 7-amino-4-methylcoumarin (AMC) labeled peptide is widely used as a fluorogenic substrate for 20S proteasome, calpains and other chymotrypsin-like proteases. Cleavage of this AMC peptide by the enzymes generates strongly fluorescent AMC that is monitored fluorimetrically at Abs/Em=353/442 nm.
Sigma Aldrich Fine Chemicals Biosciences Hippuryl-Arg carboxypeptidase substrate | 744-46-7 | MFCD00055706 | 250MG
Hippuryl-Arg carboxypeptidase substrate | Purity: >=98% (TLC) | Mol Wt: 335.36 | 744-46-7 | MFCD00055706 | 250MG
Medchemexpress LLC Calpain substrate | 728005-83-2 | 99.9% | 1675.95 | 5 MG
Calpain substrate | 728005-83-2 | 99.9% | 1675.95 | 5 MG
![Alkali Scientific D Luciferin Sodium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thia zolecarboxylic acid, sodium salt], 100 Mg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659814.jpg-250.jpg)
Alkali Scientific D Luciferin Sodium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thia zolecarboxylic acid, sodium salt], 100 Mg
D-Luciferin Sodium Salt is a key substrate used in bioluminescence assays, particularly for detecting the activity of luciferase in biological systems. This reagent, in its sodium salt form, is used in laboratory applications to measure light emission through a reaction with the enzyme luciferase. The product is commonly used in applications such as gene expression studies, cell viability assays, and ATP detection. With its ability to generate light in the presence of luciferase, it provides an effective and highly sensitive method for biochemical assays. This 100mg bottle is ideal for laboratory-scale experimentation and analysis.
![Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 100 Mg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661849.jpg-250.jpg)
Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 100 Mg
D-Luciferin Free Acid is a bioluminescence substrate formulated for high-sensitivity detection in a range of biochemical assays. When oxidized by luciferase, D-Luciferin emits light, making it a valuable tool for detecting ATP levels, measuring cellular activity, or assessing enzyme activity. This 100mg product is designed for use in laboratories where precise and sensitive measurements are required. It is commonly used in applications involving molecular biology, microbiology, and cellular research, providing excellent luminescence signals for quantification and assay development in high-throughput systems.
Medchemexpress LLC D-luciferin sodium | 103404-75-7 | MFCD00044938 | 99.9% | 302.30 | C11H7N2NaO3S2 | 5MG
D-Luciferin sodium is the sodium salt of D-luciferin, a bioluminescent substrate for firefly luciferase. Upon oxidation in the presence of luciferase, ATP, and oxygen it produces a yellow-green light (peak ~560 nm), making it suitable for sensitive reporter assays and bioluminescence imaging. Supplied as a light-yellow solid with high purity and a defined molecular weight for consistent assay performance.
- High purity for reliable assay results.
- Generates a strong yellow-green bioluminescent signal (peak ~560 nm).
- Suitable for in vitro and in vivo luciferase reporter assays.
- Sodium salt form for good aqueous solubility and easy preparation.
- Supplied in small, accurately weighed quantities for precise dosing.
Medchemexpress LLC Boc-Gln-Ala-Arg-AMC (acetate) | 00-00-0 | 99.7% | 690.74 g/mol | C31H46N8O10 | 5 MG
Boc-Gln-Ala-Arg-AMC acetate is an AMC-labeled peptide substrate for fluorescence-based protease assays. Enzymatic cleavage liberates the AMC fluorophore, producing a measurable fluorescent signal used in activity assays and screening applications.
- Fluorogenic peptide substrate for protease activity assays.
- Releases fluorescent AMC upon enzymatic hydrolysis.
- Validated for trypsin and TMPRSS2 activity measurement.
- High purity (99.7%) by HPLC.
- Available in small pack sizes suitable for assay development.