Substrates
Gold Biotechnology Inc D-Luciferin, Potassium Salt (Endotoxin Free) 1g
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
Medchemexpress LLC Solutol HS-15, 61909-81-7, 50 g
Formula: (C₂H₄O)nC₁₈H₃₆O3. Solutol HS-15 is a permeability enhancer. Store at 4°C, sealed storage, away from moisture.
Surmodics Inc TMB One Component HRP Membrane Substrate, 100mL
The BioFX™ one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution for membrane applications. The staining pattern provides a dark blue precipitate with low background to increase the signal-to-noise ratios in western blot, dot blots and line assays
Surmodics Inc BCIP/NBT Purple One Component AP Membrane Substrate, 1L
The substrate is a ready to use, one-component formulation of 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium for use in immunoblotting or other membrane applications.
Vector Laboratories ImmPACT DAB EqV Peroxidase (HRP) Substrate | 100mL
ImmPACT DAB EqV consists of equal volumes of Reagent 1 and Reagent 2, and when mixed together form a working solution. ImmPACT DAB EqV produces a dark brown reaction product and is 3 to 5 times more sensitive than the original DAB substrate.
Vector Laboratories ImmPACT AEC HRP Substrate Kit 120 ml
Vector Laboratories ImmPACT AEC Peroxidase (HRP) Substrate Kit produces red reaction products for use in IHC and blot-based protein detection protocols. The novel ImmPACT technology generates a crisper, brighter red reaction product that is 3-5 times more sensitive than conventional AEC substrates. ImmPACT AEC can be used for both manual and automated staining methods. Stained slides must be aqueously mounted, e.g. in VectaMount AQ and are stable for up to four years at 4 degrees C. This product consists of diluent and concentrated chromogen stock solutions in convenient dropper bottles and can be used on nylon or PVDF membranes. Total working volume is 120 ml. It is not recommended for use on nitrocellulose membranes.
Rockland Immunochemicals TMB ELISA PEROXIDASE SUBSTRATE - TMBE-100
TMB ELISA Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction can be halted with acid to change the TMB to yellow. TMB ELISA Peroxidase Substrate is ideal for investigators in Microbiology, Immunology, and Cancer research.
Gold Biotechnology Inc D-Luciferin, Potassium Salt (Proven and Published™) 250 mg
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
United States Biological Corporation IPTG DIOXANE FREE 5G
IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in E. coli. Solubility: Colorless, clear, complete Identity (IR): Conforms to structure pH (5%): 5.0-7.0 Dioxane: Not Detected Specific Rotation (C=1,H2O): -29° to -35° Melting Point: 109 -114.0°C Water (KF): ≤1.0% Blue-White Assay: Induces b-galactosidase in a pUC 18-containing strain. Storage and Stability: Powder is very stable at RT. Stable for 12 months after receipt. For long-term storage of reconstituted product, aliquot and store at -20°C. Further dilutions can be made in assay buffer.
Vector Laboratories ImmPACT NovaRED™ Peroxidase (HRP) Substrate Kit 120 ml
ImmPACT NovaRED™ Peroxidase (HRP) Substrate produces an intense red reaction product, with 2-4X the sensitivity of standard VECTOR NovaRED substrate. Sections stained with NovaRED can be dehydrated, cleared, and permanently mounted. This chromogen is useful as an alternative to DAB or as a second color for multiple antigen labeling. ImmPACT NovaRED Substrate also provides excellent color contrast in pigmented tissue such as melanoma and can be used for both manual and automated staining methods. ImmPACT NovaRED™ substrate produces a red reaction product for use in immunohistochemical applications. This product generates 120 ml of working solution that is stable for up to 14 days when stored at 4 degrees C.