Substrates
Enzo Life Sciences Z-YVAD-AFC (1mg). CAS: 201608-13-1
Caspase-1 substrate. Fluorogenic substrate for caspase-1. AFC has an excitation maximum of 400nm and an emission maximum of 505nm. This product is similar to Ac-YVAD-AMC, ALX-260-024, but the AFC fluorophore has a greater Stokes' shift upon cleavage and the N-terminal is blocked by a benzyloxycarbonyl moiety, which increases cell permeability. Alternative name: Caspase 1 substrate (fluorogenic). Formula: C39H40N5O11F3. MW: 811.8. Purity: ≥95% (HPLC). Single spot (TLC). Solubility: Soluble in DMSO, DMF or methanol; insoluble in water. Long Term Storage: -20°C. Use/Stability: DMF or DMSO stock solution highly stable at -20°C. Handling: Protect from light.
Sino Biological Peptide Substrates: Thr-phosphopeptide
The Thr-phosphopeptide sequence (KRT(p)IRR) is derived from human NR2F6 (amino acids 79-84) and is suitable as a substrate for PP2A, PP2B, PP2C and PP1.
Medchemexpress LLC Z-val-val-arg-AMC | 124485-41-2 | MFCD00238523 | 99.8% | 663.76 | C34H45N7O7 | 5 MG
Z-Val-Val-Arg-AMC is a fluorogenic peptide substrate used to measure cathepsin protease activity in biochemical and enzymatic assays. The peptide contains a benzyloxycarbonyl (Z) protecting group and a 7-amino-4-methylcoumarin (AMC) fluorescent leaving group; cleavage by cathepsins releases AMC and generates a measurable fluorescence signal. For research use only.
- Fluorogenic substrate for cathepsin activity assays.
- Sequence: {Z}-Val-Val-Arg-{AMC}.
- High purity (99.8%).
- Molecular weight 663.76.
- Chemical formula C34H45N7O7.
- Available in small mg-scale packages for assay development.
Enzo Life Sciences Cathepsin D & E substrate (fluorogenic) (1 mg)
Quenched fluorogenic substrate for cathepsin D (kcat/Km=1.09 x 10 M-1s-1) and cathepsin E (kcat/Km=1.56 x 10 M-1s-1). Mca fluorescence is quenched by the Dnp group until cleavage separates them. Activity of the two enzymes on this substrate can be distinguished by the use of Ascaris pepsin inhibitor, which inhibits cathepsin E, but not cathepsin D. This substrate is also cleaved by MMP-2, MMP-3, MMP-9, but not MMP-1 nor MMP-13. This substrate is useful for inhibitor screening and kinetic analysis. Ex.: 320-340 nm, Em.: 393-420 nm. Mca-Pro-Leu-OH (BML-P127) can be used to calibrate for Mca fluorescence. MW: 1756.0. Formulation: Trifluroacetate salt. Purity: ≥95% (HPLC). Sequence Mca-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 [Mca= (7-methoxycoumarin-4-yl)acetyl; Dnp=dinitrophenyl]. Appearance: Yellow powder. Solubility: Soluble in DMSO (1mM) or 50% acetonitrile (10mg/ml). Long Term Storage: -20°C. Handling: Protect from light. Keep cool and dry.
Biotium NucView 530 Caspase-3 Substrate, 1 mM in DMSO, Trial Size
NucView Caspase-3 Substrates are novel fluorogenic substrates for real-time or endpoint detection of apoptosis in live cells. The non-toxic, no-wash substrates do not inhibit caspase-3 unlike other caspase substrates or FLICA assays permitting multiday analysis. Consisting of a high-affinity DNA dye linked to a DEVD caspase-3 substrate moiety, the cell permeable non-fluorescent substrate is cleaved by caspase-3 in apoptotic cells to emit fluorescence, allowing detection of both caspase-3 activity and apopototic nuclear morphology. Suitable for flow cytometry, fluorescence microscopy, or live cell imaging systems, staining is compatible with fixation and permeabilization for immunostaining. NucView 488 is validated in over 100 cell types and 200 publications. Available with blue (NucView 405), green (NucView 488), or red (NucView 530) fluorescence, in DMSO or PBS for DMSO-sensitive cells, and as kits with apoptosis or necrosis probes. NucView technology is covered by U.S. patents.
Bioworld 4-Methylumbelliferyl-A-L-Iduronide, 1 mg
Substrate for alpha-L-IduronidaseSpecification: * Slightly soluble in water This product is sold for laboratory research use onlyReference:Use of 4-methylumbelliferryl-alpha-L-iduronide and 4-trifluoromethylumbelliferryl-alpha-L-iduronide for detecting alpha-L-iduronidase deficiencies in human tissue and for rapid prenatal diagnosis of Hurler disease. Vopr Med Khim. 1991 Jan-Feb; 37 (1):74-7.
Research Products International Corp IPTG, 200 G
IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.
Dioxane-Free
Water Content: <0.49%
Echelon Biosciences Research Labs PI(3,4,5)P3 BIOTIN 10 ug
Biotin PI(3,4,5)P3 (Biotin Phosphatidylinositol 3,4,5-trisphosphate) is a water soluble analog of PI(3,4,5)P2 (PIP3) labeled with biotin at the sn-1 position.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2. This item is non returnable
Echelon Biosciences Research Labs BODIPY FL-PI(3,4,5)P3 50 ug
BODIPY FL PI(3,4,5)P3 (BODIPY FL Phosphatidylinositol 3,4,5-trisphosphate) is a water soluble analog of PI(3,4,5)P3 (PIP3) labeled with the green fluorophore, Bodipy-FL at the sn-1 position.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2. This item is non returnable
Cayman Chemical TR-FRET asy Buffer AddItIve 1g
An ionizable cationic lipid has been used in the generation of LNPs for the delivery of mRNA in vivo LNPs containing 119-23 and encapsulating an mRNA reporter show greater intramuscular transfection efficacy compared to LNPs containing SM-102 or DLin-MC3-DMA in mice 119-23-containing LNPs accumulate in mouse hepatic and splenic immune cells to a greater extent than SM-102-containing LNPs LNPs containing 119-23 and encapsulating mRNA encoding hEPO increase serum hEPO levels in mice
Biotium Aquaphile™ Coelenterazine h, lyophilized solid
Aquaphile coelenterazine native is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use.Properties: Yellow solid soluble in water or PBS buffer; Store at -20°C and protect from light; C26H21N3O3; MW: 423; [55779-48-1].
American Research Products Inc AntiHuman HER2
Anti-Human HER-2 (Trastuzumab) - HRP Biosimilar is a biosimilar antibody with human reactivity Available in 100ug size with 7 - 14 business days lead time Ships via gel pack Functional grade preclinical antibodies may be stored sterile as received at 2-8 C for up to one month For longer term storage aseptically aliquot in working volumes without diluting and store at -80 C Avoid Repeated Freeze Thaw Cycles
![eMolecules AstaTech / D-LUCIFERIN POTASSIUM SALT / 0.25g / 233637367 / TD8084 / 95.000 / 115144-35-9 / [null] / 318.410 / C11H7KN2O3S2](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502469431.JPG-250.jpg)
eMolecules AstaTech / D-LUCIFERIN POTASSIUM SALT / 0.25g / 233637367 / TD8084 / 95.000 / 115144-35-9 / [null] / 318.410 / C11H7KN2O3S2
AstaTech / D-LUCIFERIN POTASSIUM SALT / 0.25g / 233637367 / TD8084 / 95.000 / 115144-35-9 / [null] / 318.410 / C11H7KN2O3S2
Neogen Diagnostics K-Blue Aqueous TMB Substrate, 200 mL, Microwell, HRP, Colorimetric, solvent-free
K-Blue Aqueous is a revolutionary 100% solvent-free, non-volatile TMB substrate. It contains 3,3,5,5 tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) in a one-bottle format with long-term stability and high activity. K-Blue Aqueous develops a deep blue color in the presence of peroxidase labelled conjugate and is ideal for end users working under strict regulatory requirements. The formula is compliant with Annex XVII for REACH and contains no hazardous substances at or above published reporting levels. K-Blue Aqueous has been optimized for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate. Stable for a minimum of 36-months when stored at 2-8C.
CPC Scientific N-Methylsulfonyl-DPhe-Gly-Arg-pNA (trifluoroacetate salt) 5MG
Determination of t-PA(tissue plasminogen activator) in purified preparation and in cell culture supernatents in the micro gram scale. Working concentration ~0.25 mM.ONE-LETTER SEQUENCE: N-Methylsulfonyl-DPhe-GR-pNAMOLECULAR FORMULA: C24H32N8O7S1MOLECULAR WEIGHT:576.5STORAGE CONDITIONS: -20 5CRESEARCH AREA: Apoptosis