Substrates
Sigma Aldrich Fine Chemicals Biosciences D Luciferin synthetic100MG
D-Luciferin is a small molecule present abundantly in bioluminescent organisms. This molecule is sensitive to oxygen and light.
Medchemexpress LLC HER2/CD340 Human H 10ug
The HER2/CD340 protein is a dynamic tyrosine kinase that is essential in the neuregulin receptor complex and regulates microtubule dynamics Upon activation it triggers the MEMO1-RHOA-DIAPH1 pathway inhibits GSK3B and promotes the association of APC and CLASP2 on the cell membrane to achieve microtubule stabilization HER2/CD340 Protein Human (HEK293) is the recombinant human-derived HER2/CD340 protein expressed by HEK293 with tag free
Bioworld IPTG, 2 g
IPTG Isopropyl-β-D-Thiogalactopyranoside. An inducer of β-galactosidase used to promote the expression of proteins in cells controlled by the lac operator system. For blue/white screening, 0.1mM final IPTG concentration in LB (Luria Broth) media is recommended. Techncial Information:Biotechnology Grade.Dioxane free.
Gold Biotechnology Inc X-gluc (CHX salt) 2 x 1 g
X-Gluc is a chromogenic substrate for beta-glucuronidase (GUS) which is encoded by the gusA (uid A) gene, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate. X-Gluc is often used in GUS staining of plant cells and tissue and in infections caused by E. coli.
Enzo Life Sciences OMNICATHEPSIN fluorogenic substrate (10mg). CAS: 65147-22-0
Fluorogenic substrate cleaved by plasma kallikrein and kallikrein 8, but not plasmin; by papain; and by most cathepsins (cathepsins B, C/DPP-I, F, K/O2, L, L2/V, O, S, and X/Z). Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144). Alternative name: Z-FR-AMC, Z-Phe-Arg-AMC. Purity: ≥99%. Long Term Storage: -20°C.
Medchemexpress LLC HER2/CD340 Human S 10ug | 10ug
The HER2/CD340 protein is a dynamic tyrosine kinase that is essential in the neuregulin receptor complex and regulates microtubule dynamics Upon activation it triggers the MEMO1-RHOA-DIAPH1 pathway inhibits GSK3B and promotes the association of APC and CLASP2 on the cell membrane to achieve microtubule stabilization HER2/CD340 Protein Human (S310Y HEK293 His) is the recombinant human-derived HER2/CD340 protein expressed by HEK293 with C-10 His labeled tag and S310Y mutation
Sigma Aldrich Fine Chemicals Biosciences Adenosine 5'-phosphosulfate sodium salt >=85% | 102029-95-8 | MFCD00057011 | 5MG
Adenosine 5'-phosphosulfate sodium salt >=85% | Purity: >=85% | Mol Wt: 427.28 | 102029-95-8 | MFCD00057011 | 5MG
Medchemexpress LLC D-luciferin potassium | 115144-35-9 | 100.0% | 318.41 | C11H7KN2O3S2 | 250MG
D-Luciferin potassium is the potassium salt of D-luciferin, the natural substrate for firefly luciferase. In the presence of ATP and appropriate cofactors it undergoes enzymatic oxidation to produce rapid, proportional chemiluminescent light, making it suitable for reporter gene assays, cell viability measurements, and sensitive ATP detection. Supplied as a high-purity solid and available in multiple pack sizes for research use.
- High-purity potassium salt for reliable luminescent assays.
- Produces rapid, proportional chemiluminescent signal.
- Compatible with luciferase-based reporter and ATP assays.
- Supplied as a solid, stable form for storage and handling.
- Available in a range of pack sizes to suit experimental needs.
Biotium IPTG, 5 g
IPTG (Isopropylthio-β-galactoside) is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures. Properties: White solid soluble in DMSO; Store desiccated at 4°C and protect from light; C9H18O5S; MW: 238.3
Ubpbio SUC-LLVY-AMC, 25MG
Fluorescent Peptides/Protein Substrates – SUC-LLVY-AMC (UBPBio catalog# G1100, G1101) – Suc-LLVY-AMC is a fluorogenic substrate for the chymotrypsin – like activity of the 20S and 26S proteasomes. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.