Substrates
Echelon Biosciences Research Labs Plasminogen Activator Substrate - 10 mg,
Plasminogen Activator Substrate - 10mg , BOC-Val-Gly-Arg-beta-naphthylamide Category/Application: Enzyme Substrates This item is non returnable
Echelon Biosciences Research Labs Z-VVR-AFC - 5 mg, CAS#: 124485-41-2
Z-VVR-AFC - 5mg , Z-Val-Val-Arg-AFC Category/Application: Enzyme Substrates This item is non returnable
Echelon Biosciences Research Labs Ac-Arg-Ala-Val-AMC - 5 mg,
Ac-Arg-Ala-Val-AMC - 5mg , Ac-Arg-Ala-Val-AMC Category/Application: Enzyme Substrates This item is non returnable
Echelon Biosciences Research Labs Succinyl-Phe-Ala-His-Phe-AMC - 5 mg,
Succinyl-Phe-Ala-His-Phe-AMC - 5mg , Succinyl-Phe-Ala-His-Phe-AMC Category/Application: Enzyme Substrates This item is non returnable
Enzo Life Sciences FLUOR DE LYS H4-AcK16 deacetylase substrate (0.5µmol)
The deacetylated state of lysine-16 of histone H4 (H4-K16) is thought to be a critical factor in determining regions of chromatin silencing. Conversely, acetylation of lysine-16 can be associated with increased transcription, such as that due to dosage compensation from the X-chromosome of male Drosophila. The acetylated H4 lysine-16 is preferentially deacetylated by yeast Sir2 in vitro and in vivo. FLUOR DE LYS-H4-AcK16 is a fluorogenic, acetylated peptide substrate suitable for various lysine deacetylases, including the HDAC activities in HeLa nuclear extract, HDAC8, SIRT1 (human Sirtuin 1) and SIRT2 (human Sirtuin 2). It is based on residues from histone H4 (12-16, Lys-Gly-Gly-Ala-Lys(Ac)) and was the preferred substrate for both SIRT1 and SIRT2 within a group patterned on acetylation sites in histone H3 and histone H4 N-terminal tails. Purity: ≥95%. Formulation: Supplied as a 5 mM solution (100 µl) in HDAC Assay Buffer.
Enzo Life Sciences TACE substrate II (fluorogenic) (100µg)
Sensitive fluorogenic substrate for TACE (TNFα converting enzyme, ADAM17). Also cleaved by ADAM10 and likely other ADAMs and MMPs. The kcat/Km of TACE for this substrate is 7-fold higher than for the same peptide with the Mca-Dpa fluorophore-quencher pair. FAM fluorescence is quenched by the TAMRA group until cleavage separates them. Ex.: 485nm, Em.: 535nm, although the following Ex/Em can also be used: 490,494/515,520nm. This substrate is useful for inhibitor screening, kinetic analysis, and cellular activity assay. Alternative name: ADAM17 substrate 2 (fluorogenic). Purity: ≥95% (HPLC). Formulation: Lyophilized. Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Enzo Life Sciences Z-RR-AMC (50mg). CAS: 88937-61-5
Fluorogenic substrate specific for cathepsin B (kcat/Km=105 M-1s-1). The following proteases do not cleave, or inefficiently cleave, this substrate: papain, cathepsins C/DPP-I, F, H, K/O2, L, L2/V, O, S, X/Z. Ex.: 365 nm, Em.: 440 nm, although the following Ex/Em can also be used: 355,380/450,460. This substrate is useful for analysis of cathepsin B activity in cell extracts, inhibitor screening, and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144). Alternative name: Z-Arg-Arg-AMC. Purity: ≥98%. Long Term Storage: -20°C.
Enzo Life Sciences OMNICATHEPSIN fluorogenic substrate (50mg). CAS: 65147-22-0
Fluorogenic substrate cleaved by plasma kallikrein and kallikrein 8, but not plasmin; by papain; and by most cathepsins (cathepsins B, C/DPP-I, F, K/O2, L, L2/V, O, S, and X/Z). Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144). Alternative name: Z-FR-AMC, Z-Phe-Arg-AMC. Purity: ≥99%. Long Term Storage: -20°C.
Sigma Aldrich Fine Chemicals Biosciences N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin >=97% (HPLC), powder | 169332-61-0 | MFCD00671411 | 5MG
N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin >=97% (HPLC), powder | Purity: >=97% (HPLC) | Mol Wt: 675.64 | 169332-61-0 | MFCD00671411 | 5MG
Enzo Life Sciences MeOSuc-AAPV-pNA (5mg). CAS: 70967-90-7
Highly sensitive substrate for human and mouse neutrophil elastase (leukocyte elastase) and neutrophil proteinase 3 (PR-3, myeloblastin), but not cathepsin G nor chymotrypsin. This substrate can be used for in vitro assays with purified enzyme, or with biological fluids or conditioned medium. Hydrolysis of the substrate can be detected at 405-410 nm. Purity: ≥98%. Solubility: Soluble in DMSO (>20 mM) or methanol (1 mg/mL). Long Term Storage: -20°C.
Enzo Life Sciences CSH103 substrate (500µg). CAS: 135546-44-0
This sequence, ADAQHATPPKKKRKVED, is SV40 L Tag (118-138) with Ser to Ala120 and Ala123 substitutions to increase specificity. Substrate is specific for p34cdc2 (CDK1) and is a poor substrate for CKI, II, PKA, GSK3, PKC, CaMKII, and phosphorylase b kinase. Can be used in cell lysates using TCA/phosphocellulose assay. Km=74 µM for active complex with cyclin B. Long Term Storage: -20°C.
Research Products International Corp BCIP, P-Toluidine Salt, 500 MG
- Substance Name: 5-Bromo-4-Chloro-3-Indolyl-Phospate-P-Toluidine Salt
- CAS Number: 6578-06-9
- Molecular Formula: C8H5BrCINO4P • C7H10N
- Molecular Weight: 433.6
![Research Products International Corp Magenta-Gal [5-Bromo-6-chloro-3-indolyl-B-D-galactopyranoside], 100 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B73000-0.1.JPG-250.jpg)
Research Products International Corp Magenta-Gal [5-Bromo-6-chloro-3-indolyl-B-D-galactopyranoside], 100 Milligrams
Chromogenic substrate of β-Galactosidase. Produce magenta color.
![Research Products International Corp D-Luciferin Sodium Salt [4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thia-zolecarboxylic acid, sodium salt], 100 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-L37080-0.1.JPG-250.jpg)
Research Products International Corp D-Luciferin Sodium Salt [4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thia-zolecarboxylic acid, sodium salt], 100 Milligrams
RPI offers the highest purity D-Luciferin Sodium Salt for a wide range of bioluminescent applications. Allows highly sensitive bioluminescent measurements. Ideal for reporter gene assays, ATP assays, whole animal studied, DNA sequencing and more. Our product is specially purified and tested extensively to ensure the highest signal and reproducibility with low background and sensitivity. Guaranteed purity is determined by both standard HPLC and Chiral HPLC methods. Protect from light and moisture.
Echelon Biosciences Research Labs Capsase Substrate - 5 mg, CAS#: 219138-08-6
Capsase Substrate - 5mg , Z-Val-Asp-Val-Ala-Asp-AFC Category/Application: Enzyme Substrates This item is non returnable