Substrates
![Alkali Scientific D Luciferin Sodium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thia zolecarboxylic acid, sodium salt], 1 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660983.jpg-250.jpg)
Alkali Scientific D Luciferin Sodium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thia zolecarboxylic acid, sodium salt], 1 G
D-Luciferin Sodium Salt (1 g) is a key reagent for bioluminescence assays, commonly used to detect luciferase enzyme activity. This compound is widely used in biochemical and molecular biology applications, where it reacts with luciferase to emit light, enabling the quantification of enzyme activity. The high concentration in this 1 g bottle is suitable for extended or large-scale experiments, including cellular assays, gene expression analysis, and ATP quantification. The bioluminescence generated through this reaction offers an effective and sensitive method for detecting and quantifying luciferase activity in research and diagnostics.
Medchemexpress LLC Z-Gly-Pro-AMC | 68542-93-8 | 99.8% | 463.48 g/mol | C25H25N3O6 | 10 MG
Z-Gly-Pro-AMC is a fluorogenic peptide substrate used to measure prolyl endopeptidase activity. Enzymatic hydrolysis releases the fluorescent 7-amino-4-methylcoumarin (AMC), enabling sensitive fluorometric assays.
- Hydrolyzed by prolyl endopeptidase to release 7-amino-4-methylcoumarin (AMC).
- Excitation 380 nm, emission 465 nm for fluorometric detection.
- High purity: 99.8%.
- Molecular weight 463.48 g/mol; molecular formula C25H25N3O6.
- Available as a 10 mg vial for laboratory assays.
- Suitable for enzymatic activity assays and fluorescence-based screening.
Zymo Research Corporation 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL) (5 x 5 ml)
Ready-to-use sterile 2% X-GAL solution that can be added directly to your agar plates. The X-GAL solution has been uniquely formulated to reduce false positives during blue-white screening and generates a rich blue color that is easily distinguishable from background.
Sigma Aldrich Fine Chemicals Biosciences Azocasein protease substrate | 102110-74-7 | MFCD00130592 | 10g
Azocasein protease substrate | 102110-74-7 | MFCD00130592 | 10g
Sigma Aldrich Fine Chemicals Biosciences Azocasein | 102110-74-7 | MFCD00130592 | 5g
Azocasein | 102110-74-7 | MFCD00130592 | 5g
EPICYPHER INC EPIDYNE-FRET NUCLEOSOME REMODE
EpiDyne-FRET Nucleosome Remodeling Assay Substrate 50ugMononucleosomes assembled from recombinant human histones expressed in E. coli
AG Scientific Inc X-GAL and IPTG Solution, 50 ML
Stable solution of X-Gal and IPTG. Does not contain DMSO or DMF.IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.Solution concentration: 10 mg/mL X-Gal, 8 mg/mL IPTG.Not for human therapeutic use or for medicinal purposes. For research applications only.CAS Number: 7240-90-6, 367-93-1Molecular Weight: 408.6 Da / 238.3 DaChemical Formula: C14H15BrClNO6 / C9H18O5SSolubility: WaterStorage Temperature: +4C
AG Scientific Inc IPTG, 100 G
IPTG known as Isopropyl-Beta-D-Thiogalactopyranoside functions as an inducer of galactosidase activity by binding to and inhibiting the repressor.It is utilized for the induction of expression from the lac promoter and derivates. IPTG is also used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene.CAS Number: 367-93-1Molecular Weight: 238.3 DaChemical Formula: C9H18O5SSolubility: Soluble in water (5%), renders clear colorless solutionStorage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.
Apexbio Technology LLC D-Luciferin (potassium salt) 115144-35-9 1g
D-Luciferin (potassium salt) a potassium salt derivative of D-luciferin serves as the bioluminescent substrate for firefly luciferase The enzyme-mediated oxidation of D-luciferin in the presence of ATP and oxygen produces bioluminescent emission at approximately 560 nm without the need for external excitation This property is applied extensively in bioluminescence imaging (BLI) experiments including live-cell and small-animal imaging Cells genetically engineered to express luciferase utilize D-luciferin to generate optical signals facilitating non-invasive visualization and tracking of biological events such as tumor growth gene expression dynamics in vivo distribution and therapeutic responses