Substrates
Seracare Life Sciences Inc TMB MEMBRANE HRP SUBSTRATE
Most sensitive chromogenic substrate for peroxidase detection. It develops a deep blue color at the reaction site in the presence of peroxidase-labeled conjugates. Recommended for membrane assays and dot blots. 3-component format of 3, 3', 5, 5' - Tetramethylbenzidine, simply mix and use.
Seracare Life Sciences Inc TRUEBLUE HRP SUBSTRATE
TrueBlue is up to 100 times more sensitive than DAB and it is free of known carcinogens. The strong blue reaction product provides excellent contrast with DAB and other substrates. HistoMark TrueBlue provides the most sensitive means available to detect peroxidase-labeled conjugates. Because of its high sensitivity, TrueBlue is recommended for detection of the least abundant marker in immunohistochemistry applications. TrueBlue is alcohol and xylene insoluble.
AdipoGen L-Leucine-7-amido-4-methylcoumarin hydrochloride
L-Leucine-7-amido-4-methylcoumarin hydrochloride, AdipoGen Life Sciences. Chemical. CAS: 62480-44-8. Formula: C16H20N2O3 . HCl. MW: 324.8. Synthetic. Fluorogenic substrate for leucine aminopeptidase. Sensitivity of fluorescence detection is greatly increased over other methods such as 4-Methoxy-naphthylamide (pNA) assays. 62480-44-8
Biotium Coelenterazine 400a (DeepBlueC™)
Coelenterazine 400a, also called DeepBlueC™ is a coelenterazine derivative that serves as a substrate for Renilla luciferase (Rluc) and generates an emission peak centered around 400 nm. It is the preferred Rluc substrate for BRET studies because it has minimal interference with the emission of the GFP acceptor. Properties: Yellow solid soluble in MeOH or EtOH; Store at 20°C and protect from light; C26H21N3O; MW: 391.46. DeepBlueC is a trademark of BioSignal Packard.
Sino Biological Peptide Substrates: IKKtide
The IKKtide peptide sequence (KKKKERLLDDRHDSG-LDSMKDEE) is derived from human IkBA (amino acids 21-41) and is suitable as a substrate for IKK alpha and IKK beta.
Seracare Life Sciences Inc TMB SUBSTRATE SOL B 1X100ML
Most sensitive chromogenic substrate for peroxidase detection, more sensitive than ABTS and OPD. It develops a deep blue color in the presence of peroxidase-labeled conjugates measurable at 650 nm. Turns yellow when stopped by acidification and read at 450 nm. Recommended for ELISA and blotting applications. 2-component format of 3, 3', 5, 5' - Tetramethylbenzidine, simply mix and use. Has longer shelf life than 1-component TMB substrates.
Neogen Diagnostics K-Blue Aqueous TMB Substrate, 500 mL, Microwell, HRP, Colorimetric, solvent-free
K-Blue Aqueous is a revolutionary 100% solvent-free, non-volatile TMB substrate. It contains 3,3,5,5 tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) in a one-bottle format with long-term stability and high activity. K-Blue Aqueous develops a deep blue color in the presence of peroxidase labelled conjugate and is ideal for end users working under strict regulatory requirements. The formula is compliant with Annex XVII for REACH and contains no hazardous substances at or above published reporting levels. K-Blue Aqueous has been optimized for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate. Stable for a minimum of 36-months when stored at 2-8C.
Bioworld TMB Stop Reagent, 450 nm, 100 mL
TMB Stop ReagentStop reagent is 0.16M sulfuric acid used to stop the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB) reaction. Horseradish peroxidase (HRP) enzyme conjugates react withTMB and peroxide to produce a blue byproduct having maximum absorbance at 605nm. Adding Stop reagent changes it to yellow color with absorption maximum at 450 nm and stabilizes the color development.Quantity: 100ml
![Research Products International Corp D-Luciferin Sodium Salt [4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thia-zolecarboxylic acid, sodium salt], 250 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-L37080-0.253.JPG-250.jpg)
Research Products International Corp D-Luciferin Sodium Salt [4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thia-zolecarboxylic acid, sodium salt], 250 Milligrams
RPI offers the highest purity D-Luciferin Sodium Salt for a wide range of bioluminescent applications. Allows highly sensitive bioluminescent measurements. Ideal for reporter gene assays, ATP assays, whole animal studied, DNA sequencing and more. Our product is specially purified and tested extensively to ensure the highest signal and reproducibility with low background and sensitivity. Guaranteed purity is determined by both standard HPLC and Chiral HPLC methods. Protect from light and moisture.
Enzo Life Sciences Cathepsin D & E substrate (fluorogenic) (1 mg)
Quenched fluorogenic substrate for cathepsin D (kcat/Km=1.09 x 10 M-1s-1) and cathepsin E (kcat/Km=1.56 x 10 M-1s-1). Mca fluorescence is quenched by the Dnp group until cleavage separates them. Activity of the two enzymes on this substrate can be distinguished by the use of Ascaris pepsin inhibitor, which inhibits cathepsin E, but not cathepsin D. This substrate is also cleaved by MMP-2, MMP-3, MMP-9, but not MMP-1 nor MMP-13. This substrate is useful for inhibitor screening and kinetic analysis. Ex.: 320-340 nm, Em.: 393-420 nm. Mca-Pro-Leu-OH (BML-P127) can be used to calibrate for Mca fluorescence. MW: 1756.0. Formulation: Trifluroacetate salt. Purity: ≥95% (HPLC). Sequence Mca-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 [Mca= (7-methoxycoumarin-4-yl)acetyl; Dnp=dinitrophenyl]. Appearance: Yellow powder. Solubility: Soluble in DMSO (1mM) or 50% acetonitrile (10mg/ml). Long Term Storage: -20°C. Handling: Protect from light. Keep cool and dry.
Medchemexpress LLC HY-12591A 50mg Medchemexpress, D-Luciferin D-(-)-Luciferin;Firefly luciferin CAS:2591-17-5 Purity:98%
Medchemexpress,HY-12591A 50mg D-Luciferin D-(-)-Luciferin;Firefly luciferin CAS:2591-17-5 Formula:C11H8N2O3S2 D-luciferin is the natural substrate of luciferases that catalyze the production of light in bioluminescent insects. Purity:98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Research Products International Corp 4-Methylumbelliferyl-B-D-Galactopyranoside-6-Sulfate, Sodium Salt, 25 Milligrams
Fluorescent substrate for Morquio A Sulphatase.
- 4-Methylumbelliferone: <100PPM
MEDILUMINE INC MediLumine Inc. D-Luciferin Potassium Salt, 1 g
Highly pure D-Luciferin potassium salt. This potassium salt is a water soluble form of D-Luciferin. D-Luciferin is a common bioluminescent substrate used for imaging of firefly luciferase expression with peak chemiluminescent emission at 560 nm. The ATP dependant luciferin oxidation is catalyzed by the Luciferase enzyme encoded by luc gene.