Substrates
Research Products International Corp X-GAL and IPTG Ready to Use Non-Toxic Solution 10mg/ml, 500 Milligrams
Stable solution of X-GAL and IPTG. Does not contain DMSO or DMF. Solution concentration is 10 mg/ml X-Gal and 8 mg/ml IPTG.
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. WARNING! This product can expose you to 1-Methyl-2-Pyrrolidon, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.
Vector Laboratories ImmPACT™ VIP Peroxidase (HRP) Substrate Kit 120 ml
Vector Laboratories ImmPACT VIP Peroxidase (HRP) Substrate Kit produces an intense violet (purple) reaction that is 2-4 times more sensitive than that of Vector VIP Substrate. The Vector ImmPACT VIP chromogen provides an excellent color contrast in pigmented tissues such as melanoma or retina and can be used for both manual and automated staining methods. This kit consists of 120 ml of diluent and concentrated stock solutions in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4 degrees C. Stained sections should be dehydrated, cleared, and permanently mounted.
Research Products International Corp 4-Methylumbelliferyl-N-acetyl-B-D-glucosaminide, 100 MG
Fluorescent substrate for N-Acetyl-B-D-glucosaminidase and B-N-Acetyl-D-hexosaminidase. Ultrasensitive. Checked by actual enzymatic assay.
Research Products International Corp D-Luciferin Free Acid 500 MG
Used with firefly luciferase in bioluminescence for the determination of ATP.
AURORA BIOLABS LLC TR-FRET PARP2 Trapping Assay Kit, 384 rxn
TR-FRET PARP2 Trapping Assay Kit, 384 rxn
Vector Laboratories AEC Peroxidase (HRP) Substrate Kit 300 ml
AEC Peroxidase (HRP) Substrate Kit (3-amino-9-ethylcarbazole) produces a red reaction product that can be used as a single label or as a second color for multiple antigen labeling using either manual or automated IHC staining methods. Stained slides must be aqueously mounted (e.g. in VectaMount AQ). This kit contains stock solutions in convenient dropper bottles, including all of the reagents necessary to prepare about 300 ml of working solution (2-3000 sections). It can be also used for staining on blots. Vector Laboratories horseradish peroxidase (HRP) substrate kits include options that are cost-effective (e.g. standard DAB), sensitive (e.g. ImmPACT™ DAB) and convenient (ImmPACT DAB EqV). The range of vibrant colors available facilitates multiplex staining.
Biotium NucView 488 Caspase-3 Substrate, 1mM in 1X PBS
NucView Caspase-3 Substrates are novel fluorogenic substrates for real-time or endpoint detection of apoptosis in live cells. The non-toxic, no-wash substrates do not inhibit caspase-3 unlike other caspase substrates or FLICA assays permitting multiday analysis. They consist of a high-affinity DNA dye linked to a DEVD caspase-3 substrate moiety. Initially non-fluorescent, the cell permeable substrate is cleaved by caspase-3 in apoptotic cells and emits fluorescence, for detection of caspase-3 activity and apopototic nuclear morphology. Suitable for flow cytometry, fluorescence microscopy, or live cell imaging systems, staining is compatible with fixation and permeabilization for immunostaining. NucView 488 is validated in over 100 cell types and 200 publications. Available with blue (NucView 405), green (NucView 488), or red (NucView 530) fluorescence, as PBS solutions for DMSO-sensitive cells, and as stand-alone assays or in kits with other apoptosis or necrosis probes.
Revvity Health Sciences Inc LANCE Ultra ULight Anti-GST Antibody, 1nmole
LANCE Ultra ULight Anti-GST Antibody, 1nmole
Sigma Aldrich Fine Chemicals Biosciences SIGMAFAST(TM) OPD tablet | 50SET
SIGMAFAST(TM) OPD tablet | 50SET
![Research Products International Corp NBT [Nitro Blue Tetrazolium] [2,2'-Di-P-Nitrophenyl-5, 5'-Diphenyl-3, 3'-(3,3'-Dimethoxy-4, 4'-Diphenylene)-Ditetrazolium Chloride], 100 MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-N66000-0.1.JPG-250.jpg)
Research Products International Corp NBT [Nitro Blue Tetrazolium] [2,2'-Di-P-Nitrophenyl-5, 5'-Diphenyl-3, 3'-(3,3'-Dimethoxy-4, 4'-Diphenylene)-Ditetrazolium Chloride], 100 MG
Colorimetric substrate for dehydrogenases and other oxidases. NBT is used with X-Phos for colorimetric detection of alkaline phosphatase activity in blotting, immunohistochemical and cytochemistry techniques. Protect from light.
Research Products International Corp 4-Nitrophenyl-B-D-Glucopyranoside, 5 Grams
Spectrophotometric substrate for detection of β-D-Glucosidase.
Free PNP: <200ppm
Abcam Nitrocefin, Chromogenic cephalosporin beta-lactamase substrate, 5MG
MW 516.5 Da, Purity >95%. Chromogenic cephalosporin β-lactamase substrate. Indicates β-lactamase activity via a red color intensely proportional to the original concentration of Nitrocefin. Undergoes rapid distinctive color change from yellow (max at pH 7.0 = 390 nm) to red (max at pH 7.0 = 486 nm) as the amide bond in the beta-lactam ring is hydrolyzed.
The product is subject to the following: Abcam Restricted Use Statement
Abcam O x iRed™ probe, pero x idase fluorogenic substrate, 5MG
MW 257.24 Da, Purity >99%. Highly sensitive, stable peroxidase fluorogenic substrate used as a hydrogen peroxide probe.
The product is subject to the following: Abcam Restricted Use Statement
Research Products International Corp IPTG, 200 G
IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.
Dioxane-Free
Water Content: <0.49%