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rAPid Alkaline Phosphatase is an alkaline phosphatase isolated from bovine intestine and supplied as a recombinant enzyme of 56 kD expressed in the yeast Pichia Pastoris. The recombinant form ensures consistency and safety. rAPid Alkaline Phosphatase catalyzes the dephosphorylation of 5 phosphates from DNA and RNA nucleotides and proteins. Alkaline phosphatase treatment prevents self-ligation of DNA fragments by removing the 5-phosphoryl termini required by ligases this feature is of major importance in cloning strategies to decrease vector background. Unlike calf intestinal phosphatase rAPid Alkaline Phosphatase is rapidly completely and irreversibly inactivated by heat treatment for two minutes at 75 C. It is therefore an excellent alternative to Shrimp Alkaline Phosphatase. In addition the enzyme is active in restriction enzyme buffers. Therefore restriction enzyme digestion dephosphorylation enzyme inactivation ligation or 5-end labeling can be performed without purif
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Alkaline phosphatase catalyzes the removal of phosphate group from various compounds that are phosphorylated. Alkaline phosphatase from calf intestine hydrolyzes 5AE-monophosphate groups from both DNA and RNA. It can also hydrolyze 5AE-diphosphate and 5AE-triphosphate groups from RNA.
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The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.
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rAPid Alkaline Phosphatase is an alkaline phosphatase isolated from bovine intestine and supplied as a recombinant enzyme of 56 kD expressed in the yeast Pichia Pastoris. The recombinant form ensures consistency and safety. rAPid Alkaline Phosphatase catalyzes the dephosphorylation of 5 phosphates from DNA and RNA nucleotides and proteins. Alkaline phosphatase treatment prevents self-ligation of DNA fragments by removing the 5-phosphoryl termini required by ligases this feature is of major importance in cloning strategies to decrease vector background. Unlike calf intestinal phosphatase rAPid Alkaline Phosphatase is rapidly completely and irreversibly inactivated by heat treatment for two minutes at 75 C. It is therefore an excellent alternative to Shrimp Alkaline Phosphatase. In addition the enzyme is active in restriction enzyme buffers. Therefore restriction enzyme digestion dephosphorylation enzyme inactivation ligation or 5-end labeling can be performed without purif
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
The SMC(TM) human cTnI High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences 1) Elution buffer disrupts the sandwich separating the labeled detection antibody for quantification. 2)The Erenna and SMCxPRO(TM) immunoassay systems detect analytes using Single Molecule Counting (SMC(TM)) technology.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More