LAMP and Other Isothermal Amplification Technologies
Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.
Invitrogen™ SuperScript™ IV RT-LAMP Master Mix
Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.
Invitrogen™ Dry-Ready™ RT-LAMP Kit
Experience the simplicity and efficiency of the air drying process by gently heating materials at moderate temperatures in a simple ventilated oven.
Invitrogen™ Lyo-ready Bst DNA Polymerase
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.
Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)
Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.
Thermo Scientific™ EquiPhi29™ DNA Amplification Kit
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Thermo Scientific™ EquiPhi29™ DNA Polymerase
Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.
Thermo Scientific™ Reaction Buffer for phi29 DNA Polymerase (10X)
Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi29 DNA polymerase.
Thermo Scientific™ EquiPhi29™ DNA Polymerase Reaction Buffer (10X)
This reaction buffer is for use with EquiPhi29 DNA Polymerase. Buffer is included with purchase of the polymerase, but additional buffer is made available here to help accommodate experimental needs.
GINKGO BIOWORKS INC (3P) Ginkgo Bioworks™ Cell Free Protein Synthesis - Economy kit (E. coli CFPS): 10 mL
Ginkgo’s cell free protein synthesis (CFPS) Economy kit is formulated to maximize protein expression per dollar spent. The E. coli transcription/translation system produces proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification. The 10 mL kit enables user to run 500 reactions of 20 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C. For research use only.
Meridian Life Science Gly-Free Phi29 Polymerase -HC, 100UL
Glycerol-Free Phi29 DNA Polymerase (HC) is a highly processive DNA-dependent DNA polymerase with strong strand displacement activity, which enables efficient isothermal DNA amplification from low DNA inputs. Glycerol-Free Phi29 DNA Polymerase (HC) 3′ - 5′ exonuclease activity delivers high fidelity amplification, making it an appropriate solution for sequencing DNA template preparation. It is supplied with a 5x Reaction Buffer that contains excipients required for lyophilization. Glycerol-Free Phi29 DNA Polymerase (HC) enables flexible and scalable reaction volumes, and is suitable for applications such as rolling circle amplification (RCA) and multiple displacement amplification (MDA).
Medchemexpress LLC Phi29 DNA polymerase | 125 U
Phi29 DNA polymerase is a protein-engineered Phi29 DNA polymerase expressed in E. coli, purified and isolated multiple times. It exhibits higher amplification efficiency and sensitivity compared to the wild-type enzyme, significantly shortening reaction times.
- High amplification efficiency and sensitivity: Greatly shortens reaction time.
- Special strand displacement activity: Possesses efficient continuous synthesis characteristics.
- Strong binding to templates: Can continuously synthesize DNA fragments up to 70kb without dissociating from the template.
- Strong 3′→5′ exonuclease proofreading function: Fidelity is 100 times higher than Taq DNA polymerase.
- High purity: Multiple column purifications ensure a clear single-purpose band in SDS-PAGE gel detection, with no E. coli DNA residue or endonuclease/exonuclease contamination in qPCR detection.
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