LAMP and Other Isothermal Amplification Technologies
Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.
Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)
Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.
Invitrogen™ SuperScript™ IV RT-LAMP Master Mix
Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.
Invitrogen™ Lyo-ready Bst DNA Polymerase
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.
Invitrogen™ Dry-Ready™ RT-LAMP Kit
Experience the simplicity and efficiency of the air drying process by gently heating materials at moderate temperatures in a simple ventilated oven.
Thermo Scientific™ EquiPhi29™ DNA Amplification Kit
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
GINKGO BIOWORKS INC (3P) 10 mL CFPS Economy kit. 1000 reactions of 10 uL. Contains lysate, reagent mix, and control plasmid.
Ginkgo’s cell free protein synthesis (CFPS) Economy kit is a beta version of our CFPS kit formulated to maximize protein expression per dollar spent. The E. coli transcription/translation system produces proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification.10 mL kit enables user to run 1000 reactions of 10 µL, 200 reactions of 50 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C.
Thermo Scientific™ EquiPhi29™ DNA Polymerase
Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.
Thermo Scientific™ Reaction Buffer for phi29 DNA Polymerase (10X)
Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi29 DNA polymerase.
Thermo Scientific™ EquiPhi29™ DNA Polymerase Reaction Buffer (10X)
This reaction buffer is for use with EquiPhi29 DNA Polymerase. Buffer is included with purchase of the polymerase, but additional buffer is made available here to help accommodate experimental needs.
Mascon Medical PD-Phi29 DNA polymerase, 5000U/pk
PD-Phi29 is an engineered variant of the phi29 DNA polymerase with enhanced thermostability and activity compared to wild-type enzyme. The polymerase retains its intrinsic 3´→5' exonuclease activity, allowing high-fidelity proofreading during nucleotide incorporation. PD-phi29 is ideal for isothermal DNA amplification with exceptionally low template input (~1fg DNA). Compatible formats include RCA and MDA for whole genome amplification and plasmid sequencing.
Features:
• Thermostable up to 42°C
• High yield even from ultra-low input
• Low error rate
• Robust strand-displacement processivity
Includes two tubes (enzyme + buffer); store at –15°C to –25°C. For Research Use Only.
PD-Phi29 DNA Polymerase, 0.1 mg/mL, 10,000 U/mL, 0.5 mL (5,000 U/PK)
Mascon Medical PD-Phi29 DNA polymerase, 1000U/pk
PD-Phi29 is an engineered variant of the phi29 DNA polymerase with enhanced thermostability and activity compared to wild-type enzyme. The polymerase retains its intrinsic 3´→5' exonuclease activity, allowing high-fidelity proofreading during nucleotide incorporation. PD-phi29 is ideal for isothermal DNA amplification with exceptionally low template input (~1fg DNA). Compatible formats include RCA and MDA for whole genome amplification and plasmid sequencing.
Features:
• Thermostable up to 42°C
• High yield even from ultra-low input
• Low error rate
• Robust strand-displacement processivity
Includes two tubes (enzyme + buffer); store at –15°C to –25°C. For Research Use Only.
PD-Phi29 DNA Polymerase, 0.1 mg/mL, 10,000 U/mL, 0.1 mL (1,000 U/PK)
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