Cell Culture Media, Supplements, and Reagents
Sigma Aldrich Fine Chemicals Biosciences Beta-Estradiol | 50-28-2 | MFCD00003693 | 1mg
Beta-Estradiol | Mol Wt: 272.38 | 50-28-2 | MFCD00003693 | 1mg
STEMCELL Technologies Animal Component-Free Cell Dissociation Kit, Size: 1 Kit
Animal Component-Free Cell Dissociation Kit is optimized for dissociation and passaging of human stem and progenitor cells cultured in various media formulations. Animal Component-Free Cell Dissociation Kit includes ACF Enzymatic Dissociation Solution and ACF Enzyme Inhibition Solution. This kit was previously named MesenCult™-ACF Dissociation Kit. The name was changed to accommodate the use of this product with other cell types.
Sigma Aldrich Fine Chemicals Biosciences Burkholderia cepacia Selective Agar
Burkholderia cepacia Selective Agar | Composition (agar, 15.0 g/L; crystal violet, 0.002 g/L; lactose, 10.000 g/L; phenol red, 0.080 g/L; sodium chloride, 5.0 g/L; sucrose, 10.0 g/L; tryptone, 10.0 g/L; yeast extract, 1.5 g/L), 500g
Advanced Biomatrix Poly-D-Lysine, Solution, 0.1 mg/mL
Poly-D-Lysine comes with 50 mL of a 0.1 mg/ml sterile-filtered solution. Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule is resistant to enzymatic degradation and has been used to culture a wide variety of cell types. The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment. Poly-D-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions.
CROSSTEX INTERNATIONAL GROWTH PROMOT.BACILLUS SUBTILI
GP Sus-B. subtilis Growth Promotion Test Suspensions (QC Organisms)
VITRO BIOPHARMA MSC-GRO™ VitroPlus III Low Serum Complete Medium
VitroPlus III, Low Serum Complete, 500ml
IBI SCIENTIFIC 3P Carbenicillin Disodium Salt | 4800-94-6 | MFCD00077683 | 25 g
Carbenicillin - 25gm Carbenicillin is an antibiotic which inhibits bacterial cell wall synthesis, activity against gram-positive and gram-negative bacteria, and for cell culture media applications. Recommended use is 100mg/ml.
MilliporeSigma Milliflex Rapid Reagent Kit, 100 tests
An integral component in the Milliflex Rapid Microbiology Detection and Enumeration system, the Milliflex Rapid AutoSpray Station applies an equal volume of two reagents across a membrane surface in order to detect living microorganisms.
FUJIFILM BIOSCIENCES INC BalanCD Transfectory CHO - Liquid
BalanCD Transfectory CHO system of media and supplements is a scalable platform that produces milligram to gram-scale yields of recombinant proteins using a simplified, affordable protocol. The system (including BalanCD Transfectory CHO, Transfectory Supplement and Anti-Clumping Supplement) supports small- andLarge-scale transient transfection in CHO cells, enabling scientists to use the same medium before and after transfection.The chemically-defined, animal component-free medium produces proteinLevels equivalent to, or better than, other commercially available media.
STEMCELL Technologies STEMdiff™ Mesenchymal Progenitor Kit, Size: 1 Kit
STEMdiff™ Mesenchymal Progenitor Kit is a defined culture kit consisting of animal component-free (ACF) induction medium, expansion medium, and attachment substrate. It is optimized for the derivation of cells with mesenchymal progenitor cell (MPC)-like properties from human embryonic stem (ES) cells or induced pluripotent stem (iPS) cells. This kit provides a complete workflow of defined reagents for derivation and expansion of human ES- or iPS-derived MPCs.CollPlant (https://collplant.com/) is the manufacturer of the rhCollagen component of the cell attachment substrate.
Sigma Aldrich Fine Chemicals Biosciences Hoagland′s No. 2 Basal Salt Mixture, 1 L
Used in the preparation of Hoagland′s solution for treating root tips.
Stemculture LLC StemCultures LLC FGF2 DISCs (Standard) – 10 ng/mL in 2 mL
FGF2 DISCs are biocompatible hydrogels that release defined levels of native FGF2 stably into culture medium for one week. Native FGF2 has a short half-life (4 hours) and rapidly decays in cell culture media. Adding soluble FGF2 through daily media feeds creates fluctuations in growth factor levels that affect signaling pathways and creates a stressful cellular environment. With FGF2 DISCs, FGF2 levels are stable over one week, allowing the following reduced feeding with drastically improved cell quality and downstream applications. DISC device technology allows you to use your favorite media and feed less often. One DISC can be added per passage and can remain in the culture well/vessel for several media feeds (i.e., a pack of 48 DISCs will last 2 months when culturing in a 6 well plate). FGF2 DISCs are designed to release at a constant 10 ng/mL in 2 mL of medium. This technology gives scientists enhanced control over growth factor levels in cultures to provide high-quality cells.
Promocell GmbH PromoCell Macrophage Detachment Solution
Macrophages are strongly adherent and are not effectively dislodged from culture surfaces by standard enzymatic cell dissociation reagents. Indeed, these cells are highly sensitive to improper dissociation procedures. The PromoCell Macrophage Detachment Solution is chemically defined and non-enzymatic. It was especially designed for the gentle release of adherent macrophages and guarantees the best possible cell viability, even after prolonged exposure times. Unlike enzyme-based solutions, the PromoCell Macrophage Detachment Solution does not alter cell surface proteins and neutralization is not necessary.