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A variety of media, supplemental media components, and reagents suitable for cell culture operations and processes. Products include fully prepared media as well as antibiotics, growth factors, contamination detection and removal reagents, cell dissociation reagents, and more.
StemSpan™ CC100 cytokine cocktail contains a combination of both early- and late-acting recombinant human (rh) cytokines formulated to support the proliferation of human hematopoietic cells. It is supplied as a 100X concentrate.When added to serum-free medium, StemSpan™ CC100 promotes the expansion of CD34+ cells isolated from human cord blood and bone marrow, and is recommended if high cell yields and large numbers of CD34+ cells are desired.We recommend using StemSpan™ CC100 in combination with any of the following StemSpan™ media:• StemSpan™ SFEM (Catalog #09600)• StemSpan™ SFEM II (Catalog #09605)• StemSpan™-XF (Catalog #100-0073)• StemSpan™-AOF (Catalog #100-0130)
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pH 8.5-9.5 (25 °C), suitable for cell culture, Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP).
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Chloramphenicol - 25gm Chloramphenicol is used in the amplification of plasmids and colony hybridizations. Antibiotics dissolved in ethanol need not be sterilized.
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DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS DMEM Complete Medium contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS. DMEM Complete Medium is a convenient sterile solution for the culture of primary mammalian cells including mouse and chicken cells, and a variety of normal and transformed cells. |
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Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). Lipopolysaccharides from Escherichia coli 026:B6 was used to elicit the secretion of cytokines by human PBMC, murine bone marrow dendritic cells and rat astrocytes.
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