Additional Cell Culture Reagents and Supplements
Sigma Aldrich Fine Chemicals Biosciences GSEM Supplement 50 x, liquid, sterile-filtered, suitable for cell culture | MFCD00677096 | 100ML
GSEM Supplement 50 x, liquid, sterile-filtered, suitable for cell culture | MFCD00677096 | 100ML
Sigma Aldrich Fine Chemicals Biosciences Concanavalin A from Canavalia ensiformis (Jack bean), 11028-71-0, MFCD00071069, 5 mg
Type IV-S, lyophilized powder, aseptically processed, BioReagent, suitable for cell culture, Synonym: Con A. Concanavalin A from Canavalia ensiformis (Jack bean) has been used: in in vitro cell proliferation assay and cell cycle assessment, in lymphocyte proliferation, and in splenocyte culturing.
STEMCELL Technologies Hydrocortisone Stock Solution, Size: 3 mL
Hydrocortisone Stock Solution is recommended for use as a cell culture supplement and for the preparation of PneumaCult™, MammoCult™, and EpiCult™ media when culturing airway epithelial cells, mammary primary cells, or tumor cell lines.Hydrocortisone Stock Solution is provided at a concentration of 96 µg/mL, which is ideal as a 200X stock solution for use with PneumaCult™-ALI (Catalog #05001), MammoCult™ (Catalog #05620), EpiCult™-B (Human; Catalog #05601), and EpiCult™-C (Catalog #05630) media. It may also be used for preparing PneumaCult™-Ex Medium (Catalog #05008) or PneumaCult™-Ex Plus Medium (Catalog #05040).
Sigma Aldrich Fine Chemicals Biosciences Calcium chloride, 10043-52-4, MFCD00010903, 100 g
Linear Formula: CaCl2, Molecular Weight: 110.98, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%.
Advanced Biomatrix HyStem-C Version 2.0 Thiol-Modified Hyaluronan and Gelatin Hydrogel Kit, 2.5 mL
HyStem-C contains thiolated hyaluronic acid & gelatin for easy 3D cell culture. HyStem-C version 2.0 is reconstituted with a buffered 1X PBS. The resulting components will also result in a pH neutral isotonic salt hydrogel. The ending pH, osmo, and rheological properties are the same from Version 1.0 to Version 2.0. HyStem-C hydrogels provide an excellent starting point for optimizing the matrix for cell culture. HyStem-C is fully chemically-defined and based on three biocompatible components: thiol-modified hyaluronan (Glycosil), thiol-reactive crosslinker, PEGDA (Extralink), and thiol-modified denatured collagen (Gelin-S). Gelin-S provides basic cell-attachment sites for a wide variety of primary cells and cell lines and is therefore recommended as an ideal substrate for adherent cell types and for cell culture optimization. In some cases, HyStem-C hydrogels can be further enhanced by the addition of ECM proteins to match native signals.
Advanced Biomatrix HyStem Version 2.0 Thiol-Modified Hyaluronan Hydrogel Kit, 7.5 mL
HyStem contains thiolated hyaluronic acid for easy 3D cell culture. HyStem version 2.0 is reconstituted with a buffered 1X PBS. The resulting components will also result in a pH neutral isotonic salt hydrogel. The ending pH, osmo, and rheological properties are the same from Version 1.0 to Version 2.0. HyStem hydrogels are xeno-free and completely customizable. The hydrogel is formed when the crosslinking agent, Extralink-Lite (PEGDA) is added to a mixture of Glycosil (thiol-modified hyaluronan). Recommended for applications requiring specific attachment factor optimization as well as non-adherent cell culture, cellular applications. Extracellular matrix proteins can be mixed into the hydrogel and incorporated non-covalently before gelation. The HyStem Hydrogel Kits are optimal for culturing stem cells whose natural environment is rich in hyaluronic acid (HA).
STEMCELL Technologies ImmunoCult™ Human Th1 Differentiation Supplement, Size: 1 mL
ImmunoCult™ Human Th1 Differentiation Supplement contains a combination of human cytokines and a blocking antibody formulated to promote the robust activation, expansion, and differentiation of peripheral blood-derived, naïve, CD4+ human T cells into Th1 cells. ImmunoCult™ Human Th1 Differentiation Supplement is intended for use with ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) and ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971). This product is recommended for:• Research into the regulation of human naïve T cell differentiation and Th1 function• Development of procedures to expand and polarize naïve T cells toward a Th1 subset
STEMCELL Technologies StemSpan™ Erythroid Expansion Supplement (100X), Size: 1 mL
StemSpan™ Erythroid Expansion Supplement (100X) contains a combination of recombinant human cytokines formulated to selectively promote the expansion and differentiation of erythroid progenitor cells from CD34+ cells isolated from human cord blood (CB) or bone marrow (BM) samples.When added to serum-free medium, StemSpan™ Erythroid Expansion Supplement typically promotes the production of thousands of erythroid cells per input CD34+ cell in 14-day liquid cultures initiated with CD34+ human CB cells. See data tab for more details.StemSpan™ Erythroid Expansion Supplement (100X) is intended for use in combination with any of the following StemSpan™ media:• StemSpan™ SFEM (Catalog #09600)• StemSpan™ SFEM II (Catalog #09605)• StemSpan™-XF (Catalog #100-0073)• StemSpan™-ACF Erythroid Expansion Medium (Catalog #09860)
Sigma Aldrich Fine Chemicals Biosciences Thymidine | 50-89-5 | MFCD00006537 | 1 g
Thymidine | Purity: 99% | Mol Wt: 242.23 | 50-89-5 | MFCD00006537 | 1 g
STEMCELL Technologies ImmunoCult™ Human Treg Differentiation Supplement, Size: 1 mL
ImmunoCult™ Human Treg Differentiation Supplement contains a human cytokine and small molecule formulated to promote the robust activation, expansion, and differentiation of peripheral blood-derived, naïve, CD4+ human T cells into regulatory T cells (Tregs). ImmunoCult™ Human Treg Differentiation Supplement is intended for use with ImmunoCult™-XF T Cell Expansion Medium and ImmunoCult™ Human CD3/CD28 T Cell Activator. This product is recommended for:• Research into the regulation of human naïve T cell differentiation and Treg function• Development of procedures to expand and polarize naïve T cells into Tregs
Sigma Aldrich Fine Chemicals Biosciences Vitamin B12 BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, >=98% | 68-19-9 | 100MG
Vitamin B12 BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, >=98% | Purity: >=98% | Mol Wt: 1355.37 | 68-19-9 | 100MG
Molecular Devices CloneMatrix, Methylcellulose 2.5X Concentrate, 6 x 40 mL
CloneMatrix 2.5X is a methylcellulose concentrate used to convert standard mammalian cell culture media into semi-solid cloning media that preserves colony position for screening and automated picking.
- 2.5X methylcellulose concentrate for preparing semi-solid cloning media.
- Supplied as 6 x 40 mL bottles; each 40 mL makes 100 mL final media.
- Animal-free formulation compatible with serum-free media.
- Supports formation of discrete clonal colonies for automated colony identification and picking.
- Ready-to-dilute format reduces aliquoting and contamination risk.
ILEX LIFE SCIENCES LLC PAN Biotech HBSS Hanks Balanced Salts Solution with Ca and Mg with phenol red and NaHCO3 3000 ML 1/EA
PAN-Biotech Hanks Balanced Salts Solution (HBSS) w Ca and Mg w 0 35 g/L NaHCO3 6 x 500 ml DESCRIPTION Hanks Balanced Salts Solution (HBSS) with Phenol red with calcium and magnesium with 0 35 g/L sodium bicarbonate APPLICATION HBSS can be used as a washing solution for living cells or as a carrier solution for various substances The main purpose of HBSS is to maintain pH as well as osmotic balance and provides water and essential inorganic ions to cells It is designed for use with cells maintained in non-CO2 atmospheric conditions For cell dissociation procedures use a modified HBSS which is formulated without Ca and Mg salts BICARBONATE With 0 35 g/L Sodium Bicarbonate CALCIUM/MAGNESIUM With Ca and Mg CONCENTRATION 1x LIQUID/POWDER Liquid PHENOL RED With Phenol Red STERILE Yes STORAGE TEMP 2 C - RT MANUFACTURER PAN-Biotech GmbH COUNTRY OF ORIGIN Germany USAGE Laboratory Research Use Only Not for use in diagnostic or therapeutic procedures