Additional Cell Culture Reagents and Supplements
Invivogen Blasticidin (Solution), 500 mg (50 x 1 ml)
Purity: ≥ 95% (HPLC), Product concentration: 10 mg/ml, Blasticidin is an efficient selection antibiotic that acts on both eukaryotic and prokaryotic cells. Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery.
Sigma Aldrich Fine Chemicals Biosciences Poly-L-lysine hydrobromide mol wt 1,000-5,000 | 25988-63-0 | MFCD00237230 | 1G
Poly-L-lysine hydrobromide mol wt 1,000-5,000 | 25988-63-0 | MFCD00237230 | 1G
WHITE MOUNTAIN SYSTEMS CORPORATION RHS 32CHNL STIM/RECORD HEADSTG
NC3839066 RHS 32CHNL STIM/RECORD HEADSTG
Sigma Aldrich Fine Chemicals Biosciences Poly-L-lysine hydrobromide mol wt 1,000-5,000 | 25988-63-0 | MFCD00237230 | 500MG
Poly-L-lysine hydrobromide mol wt 1,000-5,000 | 25988-63-0 | MFCD00237230 | 500MG
ILEX LIFE SCIENCES LLC Cellendes 3-D Life Dextran-HA Hydrogel Ready-To-Design Kit (1 Kit)
3-D Life is the most flexible system for biomimetic hydrogel design for 3D cell cultures. It uses two polymers that form a hydrogel by covalent crosslinking at ambient temperature. The chemical reaction is non-toxic to cells and occurs rapidly, preventing cell settlement before gel is formed. 3-D Life Dextran-HA Hydrogel Kit enables set up of hydrogels containing hyaluronic acid (hyaluronan). Thiol groups on crosslinker HyLink form stable thioether bonds with thiol-reactive groups on SG-Dextran, resulting in gel formation. Mixed at physiological pH 7.2 for optimal cell compatibility. Slow gelation allows for manipulation before onset of gel formation. Cell adhesion peptides (RGD, Cat. No. 09-P-001) can be attached to SG-Dextran to provide a cell-adhesive matrix. Dextran hydrogels can be dissolved by dextranase (Cat. No. D10-1), allowing easy cell recovery. One kit allows formation of 3.3 ml hydrogel of a crosslinking strength of 1.2 mmol/L. Manufactured in Germany by Cellendes GmbH.
STEMCELL Technologies ClonaCell™-CHO AOF Supplement, Size: 2.5 mL
Animal origin-free, serum-free, L-glutamine-free, and phenol red-free 40X culture supplement for cloning during CHO cell line development
Sunrise Science Products CSM-Leu Powder, 10 grams
Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes. Suggested g/L: 0.69. Contents: Adenine hemisulfate (10 mg/L), L-Arginine (50 mg/L), L-Aspartic acid (80 mg/L), L-Histidine hydrochloride monohydrate (20 mg/L), L-Isoleucine (50 mg/L), L-Lysine hydrochloride (50 mg/L), L-Methionine (20 mg/L), L-Phenylalanine (50 mg/L), L-Threonine (100 mg/L), L-Tryptophan (50 mg/L), L-Tyrosine (50 mg/L), L-Valine (140 mg/L), Uracil (20 mg/L)
STEMCELL Technologies NeuroCult™ SM1 Without Antioxidants, Size: 10 mL
NeuroCult™ SM1 Without Antioxidants is an optimized serum-free culture supplement, based on the published B27 formulation (Brewer et al.), with antioxidants (tocopherol, tocopherol acetate, superoxide dismutase, catalase, and glutathione) removed. NeuroCult™ SM1 Without Antioxidants can be used in protocols where antioxidants may interfere with oxidative stress, or similar assays used in neurodegenerative research. In combination with a basal medium of choice, NeuroCult™ SM1 Without Antioxidants can be used for neural progenitor cell expansion and the study of neural stem and progenitor cell differentiation.
Sigma Aldrich Fine Chemicals Biosciences Trypan Blue solution 0.4%, for microscopy | 72-57-1 | MFCD00003969 | 250ML
Trypan Blue solution 0.4%, for microscopy | Mol Wt: 960.81 | 72-57-1 | MFCD00003969 | 250ML
Sigma Aldrich Fine Chemicals Biosciences IL 7 human Animal componen
IL7 (interleukin 7) was first identified in 1988 in a bone marrow culture as a factor that facilitated murine B cell precursors growth. It is a cytokine produced by non-lymphoid cells in lymphoid organs. In humans it is also produced by keratinocytes peripheral blood dendritic cells follicular dendritic cells intestinal epithelial cells hepatic tissue endothelial cells smooth muscle cells and fibroblasts. IL7 gene is localized to human chromosome 8q12-q13. It is a glycoprotein with a four a-helix structure with a hydrophobic core. Recombinant human IL-7 is a 17.4 kDa protein containing 153 amino acid residues.
ILEX LIFE SCIENCES LLC NEUROPAN BASAL MEDIUM 6X500ML
PAN-Biotech Neuropan Serum-Free Medium for Neuron Cultivation: Basal Medium, w: L-Glutamine, w/o: Glutamate, w: 2.2 g/L NaHCO3 (6 x 500 ml). INTRO: Neuropan Basal Medium is formulated to meet special requirements of neuronal cells. When supplemented with Neuropan-27 Supplement or Neuropan-2 Supplement, it allows long-term maintenance of normal phenotype and growth of neuronal cells, and maintains pure populations of neuronal cells without the need for an astrocyte feeder. APPLICATION: Neuropan Basal Medium meets the special cell culture requirements of prenatal and embryonic neuronal cells from hippocampus, cortex, and other regions of the brain. It allows for both long and short term maintenance of homogenous populations of neuronal cells without the need of an astrocyte feeder layer. STERILE: Yes. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. COMPOSITION: Contains salts, amino acids, glucose, L-Glutamine, and trace elements in an optimized formulation.
Sigma Aldrich Fine Chemicals Biosciences Elastase from porcine pancreas lyophilized powder, suitable for cell culture | 39445-21-1 | MFCD00130998 | 5mg
Elastase from porcine pancreas lyophilized powder, suitable for cell culture | 39445-21-1 | MFCD00130998 | 5mg
ATHENA ES L-Alanyl-L-Glutamine, dehydrated
L-Alanyl-L-glutamine is a direct replacement for L-glutamine in cell culture media. The alanyl derivative of glutamine is thermally stable, which extends the shelf-life of cell culture medium by limiting deamination of the terminal amino group of the side-chain.