Additional Cell Culture Reagents and Supplements
Cell Applications Inc ATTACHMENT FACTOR SOL 100ML
Promote attachment and spreading of endothelial cells. It is required for culturing microvascular endothelial cells 100 ml
Cell Applications Inc HBSS 100ML
Hanks Balanced Salt Solution for washing of cells before Trypsin/EDTA treatment. 100 ml
Cell Systems Corporation Passage Reagent System (4Z0-840)
The Passage Reagent System (PRS) consists of a matched set of reagents optimized for releasing cells from culture for subculture or freezing, plus Attachment Factor (4x100mL). This PRS includes PRG-1 (EDTA-dPBS Solution), PRG-2 (Trypsin/EDTA-dPBS Solution), PRG-3 (Trypsin Inhibitor-dPBS Solution) and Attachment Factor. The PRS consists of PRG-1 (EDTA-dPBS Solution), PRG-2 (Trypsin/EDTA-dPBS Solution) and PRG-3 (Trypsin Inhibitor-dPBS Solution). The EDTA in PRG-1 loosens cell attachment gently from culture surfaces. Trypsin concentration in PRG-2 is optimized to detach loosened cells while minimizing adverse effects on cell viability and surface markers. Trypsin inhibitor in PRG-3 promptly halts trypsin activity once cells have been successfully released. Attachment factor promotes cell attachment. Bulk order? Contact your Fisher Scientific sales rep. Available for next day delivery after the purchase order is received. Try Our Primary Human Cells: ideal for use with media & reagents.
Sigma Aldrich Fine Chemicals Biosciences Lipopolysaccharides from Escherichia coli O26:B6, MFCD00164401, 1 mg
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). Lipopolysaccharides from Escherichia coli 026:B6 was used to elicit the secretion of cytokines by human PBMC, murine bone marrow dendritic cells and rat astrocytes.
Sigma Aldrich Fine Chemicals Biosciences Pluronic F-127, 9003-11-6, MFCD00082049, 250G
Pluronic F-127 was used to coat a siliconized coverslip to hold an egg extract in a study. Pluronic F-127 was added to phosphate buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device. A study reports its use as a release vehicle to transport low-dose perivascular lipopolysaccharide (LPS) on mouse vein grafts. PLGA/pluronic F127 may be used to fabricate nerve guidance conduits (NGCs) for regeneration of peripheral nerve. Fabrication of poly (lactide-co-glycolide) (PLGA) — Pluronic F127 glass composites was reported. Pluronic F-127 was used for fluorescent labeling of blood vessels, astrocytes, and neurons.
InVitria ITSE Animal-Free - 100mL
InVitria’s ITS, ITSE, and ITSE+A Animal-Free are recombinant, blood-free cell culture media supplements used to optimize cell growth. For optimal proliferation and performance, cells cultured must be supplied with a critical proteins and components:Insulin: promotes uptake of glucose and amino acids, facilitating cellular signalingTransferrin: delivers intracellular iron via the transferrin receptor, drives cellular respiration, chelates free iron, and reduces generation of cytotoxic free radicals and peroxideSelenium: required for the activity of antioxidant enzymes such as glutathione peroxidase and thioredoxin reductaseEthanolamine: phospholipid precursor that improves the performance of cells in serum-free media and is required for the growth of certain cell typesAlbumin: binds, transports, and delivers ions, fatty acids, vitamins, hormones, small-molecule drugs, and toxins; regulates serum pH and osmotic pressure; and provides antioxidant activityMade in the USA.
Bioworld EGTA Buffer 0.5M, pH 8.0, 250ml
This is a metal chelating agent specific for calcium ions and is commonly used in tissue culture media and biological buffers. Specifications: Sterile solution. pH : 8, Appearance Form : Liquid, Appearance Color : Clear, Melting Point : 241 °C. 250ml
PHYTOTECHNOLOGY LABS GAMBORGS B5 MEDIUM 100L
This medium was developed for the initiation and growth of soybean cell suspensions. This medium contains no Ammonium Nitrate; it does contain Ammonium Sulfate and increased levels of Potassium Nitrate. Concentrations of NH4+ over 2 mM inhibited cell growth.
Expression Systems Production Boost Additive, 100ml
Expression Systems Production Boost Additive (PBA) is a nutrient boost designed to increase protein expression by supporting the increased needs of insect cells infected by recombinant baculovirus. PBA is a serum-free, animal-free formulation that complements ESF 921 and ESF AF insect cell culture media and extends the late and very-late expression period of production by maintaining high culture viability. PBA is a cocktail of nutrients designed for supplementation at a rate of1–10% at 6–36 hours post infection.
• Serum-free, animal-free formulation works seamlessly with ESF 921 and ESF AF
• Extends culture period by maintaining cell viability
• Increases protein expression
• Available in 100 mL or 1 L bottles
To download Instructions for Use and/or COA please visit: www.expressionsystems.com
STEMCELL Technologies NeuroCult SM1 Without Vitamin, 100 mL
Supplement 50X without vitamin A for the culture of neural progenitor cells
Cytek Biosciences Inc Monensin Solution (1000X)
Monensin Solution (1000X) ..... Cat. No. TNB-4505-L001 ..... Size 1 mL
Sigma Aldrich Fine Chemicals Biosciences Chaperonin 60 from Escherichia coli >95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder | Purity: >95% (SDS-PAGE) | MFCD00163256 | 1MG
Chaperonin 60 from Escherichia coli >95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder | Purity: >95% (SDS-PAGE) | MFCD00163256 | 1MG
InVitria Cellastim S - Recombinant Human Serum Albumin - 10g
InVitria’s Cellastim S recombinant human serum albumin (rHSA) is completely animal- and blood-free and is optimized to enhance the performance in cell culture media due to the favorable lipid profile. Cellastim S is compatible with a variety of different cell types including immune cells, VERO, MSC, HEK, CHO, etc.InVitria manufactures three animal- and blood-free rHSA components: Cellastim S, Exbumin™, and Optibumin. Cellastim S is optimized for cell culture applications. Exbumin is a regulatory approved excipient in final formulations. Optibumin is the highest-purity rHSA available on the market. Made in the USA.