Gel Electrophoresis Buffers and Diluents
Invitrogen™ Digitonin (5%)
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
Invitrogen™ NativePAGE™ Sample Buffer (4X)
A solution used to solubilize native protein samples prior to use with the NativePAGE Gel System
Corning™ 3D Clear Starter Kit
Corning™'s 3D Clear Starter Kit combines 30 mL of 3D Clear with the four buffer solutions required to process 3D tissue cultures for immunolabeling. Only immunolabels and nuclear stains need to be added. This kit comes with enough material to process three 96-well plates of 3D cell culture models.
Invitrogen™ NuPAGE™ Transfer Buffer (20X)
Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting
Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)
Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Thermo Scientific™ DNA Gel Loading Dye (6X)
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.
Invitrogen™ 4X Bolt™ LDS Sample Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Invitrogen™ NuPAGE™ Antioxidant
Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Invitrogen™ RediLoad™ Loading Buffer
Designed for easy loading and tracking of PCR products run in agarose gels
Invitrogen™ Bolt™ Transfer Buffer (20X)
Optimized for the transfer of proteins from Bolt Bis-Tris Plus gels to membranes for western blotting
Invitrogen™ NativePAGE™ Sample Prep Kit
Includes sample preparation reagents for native gel electrophoresis
Invitrogen™ NativePAGE™ Running Buffer (20X)
Used to make the NativePAGE Cathode and Anode Running Buffers
Invitrogen™ NativePAGE™ 5% G-250 Sample Additive
Add to samples prepared with the NativePAGE Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE gels
Thermo Scientific Chemicals Laemmli SDS sample buffer, reducing (6X)
Laemmli SDS sample buffer is used to prepare protein samples in the Laemmli SDS-PAGE system.