Apoptosis Reagents and Kits

BD Annexin V: FITC Apoptosis Detection Kit I Available on GSA/VA Contract for Federal Government customers only.

Staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes

BD Annexin V-PE Available on GSA/VA Contract for Federal Government customers only.

Serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis

BD Phospholipid-binding Protein, APC Annexin V Available on GSA/VA Contract for Federal Government customers only.

Has high affinity for PS, and binds to cells with exposed PS

Promega™ Caspase-Glo™ 3/7 Assay Kit

Provides a homogeneous luminescent assay that measures Caspase-3/7 activities

BD Annexin V-FITC Available on GSA/VA Contract for Federal Government customers only.

Serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis

BD Annexin V: PE Apoptosis Detection Kit I Available on GSA/VA Contract for Federal Government customers only.

Contains apoptotic program characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA

Promega™ DeadEnd™ Fluorometric TUNEL System

Detects and quantifies apoptotic cells within a cell population

eBioscience™ Annexin V Apoptosis Detection Kit eFluor™ 450 Non-distribution product as customer accommodation.

Compatible with intracellular staining. Pre-titrated and tested on mouse thymocytes cultured overnight in medium (to induce apoptosis) or on Jurkat cells treated with 10 μM Camptothecin for 4 hours. Both the Annexin V eFluor 450 and the 7-AAD components can be used at 5μL per test.

BD Annexin V: FITC Apoptosis Detection Kit II Available on GSA/VA Contract for Federal Government customers only.

Staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes.

Promega RealTime-Glo Annexin V Apoptosis and Necrosis Assay, 100 assays

A simple, non-lytic assay that allows real-time monitoring of apoptosis progression, without the need for multiple plates or complicated processing. A multimode plate reader for detecting luminescence and fluorescence is the only instrument required.

BD Violet Live Cell Caspase Probe

Cells with active caspases can be detected. BD Pharmingen™ Violet Live Cell Caspase Probe contains a fluorochrome-labeled caspase inhibitor. Fluorochrome-labeled caspase inhibitors have three functional domains: fluorochrome , 3-amino acid sequence (VAD) that binds to the active site of the activated caspase, and fluoromethyl ketone (FMK) moiety that allows the probe to irreversibly bind the active caspase.

R&D Systems™ TACS TdT In Situ Apoptosis Detection Kit, Fluorescein Available on GSA/VA Contract for Federal Government customers only.

A TUNEL assay designed for fixed cells, embedded tissue, and frozen tissue using fluorescence microscopy

R&D Systems™ CometAssay™ Single Cell Gel Electrophoresis Assay Available on GSA/VA Contract for Federal Government customers only.

Provides a simple and effective method for evaluating DNA damage in cells

eBioscience™ Annexin V Apoptosis Detection Kit APC Non-distribution product as customer accommodation.

Compatible with intracellular staining. Pre-titrated and tested on mouse thymocytes cultured overnight in medium (to induce apoptosis) or on Jurkat cells treated with 10 μM Camptothecin for 4 hours. Both the Annexin V APC and the Propidium iodide components can be used at 5μL per test

R&D Systems™ TACS TdT In Situ Apoptosis Detection Kit, Blue Label Available on GSA/VA Contract for Federal Government customers only.

A TUNEL assay designed for fixed cells, embedded tissue, and frozen tissue using light and electron microscopy

Promega™ Caspase-Glo™ 8 and Caspase-Glo™ 9 Assay Systems

Highly sensitive homogeneous luminescent assays that measure either Caspase-8 or Caspase-9 activity

R&D Systems™ TACS Annexin V-FITC Apoptosis Detection Kit Available on GSA/VA Contract for Federal Government customers only.

Facilitates rapid fluorometric quantitation of apoptotic cells

Promega™ Apo-ONE™ Homogeneous Caspase-3/7 Assay Kit

Provides a fluorescent method for the fast and sensitive measurement of active Caspase-3 and -7 in a homogeneous format

R&D Systems™ CometAssay Lysis Solution Available on GSA/VA Contract for Federal Government customers only.

For use with Single Cell Gel Electrophoresis Assay

R&D Systems™ MTT Cell Proliferation/Viability Assay Available on GSA/VA Contract for Federal Government customers only.

Safe and reliable method minimizes the number of steps necessary to assay for proliferation rate in cultured cells

R&D Systems™ CardioTACS™ In Situ Apoptosis Detection Kit Available on GSA/VA Contract for Federal Government customers only.

Provides all the reagents for the detection of DNA fragmentation in cardiac cells and tissue sections along with a cation that specifically enhances the signal in apoptotic cardiac cells

Promega™ ApoLive-Glo™ Multiplex Assay

Measures both the number of viable cells as a marker of cytotoxicity and caspase activation as a marker of apoptosis in a single well to determine the mechanism of cell death

R&D Systems™ TACS XTT Cell Proliferation/Viability Assay Available on GSA/VA Contract for Federal Government customers only.

Avoids radioactivity and gives reproducible and sensitive results

BD Apoptosis Detection Kit (APO-Direct) Available on GSA/VA Contract for Federal Government customers only.

Single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.

Promega™ DeadEnd™ Colorimetric TUNEL System

Provides rapid detection of apoptotic cells in situ at the single cell level

R&D Systems™ Calcein AM Cell Viability Assay Available on GSA/VA Contract for Federal Government customers only.

Provides a simple, rapid, and accurate method to measure cell viability and/or cytotoxicity

R&D Systems™ CometAssay LMAgarose Available on GSA/VA Contract for Federal Government customers only.

Ready-to-use in minutes with a 90 to 100°C water bath

R&D Systems™ CometSlide™ Microscope Slides Available on GSA/VA Contract for Federal Government customers only.

Sample-ready pretreated glass slides used for the rapid analysis of DNA fragmentation associated with DNA damage

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