Protein Isolation and Purification

Thermo Scientific™ SMART Digest™ Trypsin Kits

Obtain fast, simple, and highly reproducible trypsin protein digestion for peptide characterization and quantitation in biopharmaceutical protein research.

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™ Available on GSA/VA Contract for Federal Government customers only.

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

GE Healthcare HiScreen™ MabSelect™, Xtra™, SuRe™, SuRe LX™ Prepacked Columns

Designed for method optimization and parameter screening for capture of monoclonal antibodies.   GE Healthcare HiScreen™ MabSelect™, Xtra™, SuRe™, SuRe LX™ Prepacked Columns come ready-to-use  for convenience.

Triton™ X-100 (Electrophoresis), Fisher BioReagents™ Available on GSA/VA Contract for Federal Government customers only.

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO

GE Healthcare XK Chromatography Columns (Empty) Available on GSA/VA Contract for Federal Government customers only.

Ensure trouble-free operation for low to medium pressure. GE Healthcare XK Chromatography Columns (Empty) connect directly to ÄKTA design instruments or other liquid chromatography systems. The user-friendly ensures reproducible, accurate packing.

GE Healthcare PD MultiTrap™ Plates

Convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr) with high reproducibility from well to well and from plate to plate

GE Healthcare Thrombin Protease Available on GSA/VA Contract for Federal Government customers only.

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Promega Nano-Glo™ Luciferase Assay System

The Nano-Glo™ Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media.

GE Healthcare PreScission™ Protease Available on GSA/VA Contract for Federal Government customers only.

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. GE Healthcare Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

GE Healthcare PD MiniTrap™ G-25 Sample Preparation Columns Available on GSA/VA Contract for Federal Government customers only.

Use for cleanup of biomolecules with a molecular weight  >5000 Mr in sample volumes up to 0.5mL.  GE Healthcare Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

GE Healthcare HiScale™ Chromatography Columns Available on GSA/VA Contract for Federal Government customers only.

Withstand pressures of up to 20 bar (290 psi) and high flow rates. GE Healthcare Life Sciences™ HiScale™ Chromatography Columns are designed to be compatible with modern BioProcess chromatography media such as MabSelect™ and Capto™.

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin

Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation of DYKDDDDK-tagged proteins

GE Healthcare LabMate™ PD-10 Buffer Reservoirs

Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. GE Healthcare Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.

GE Healthcare Ni Sepharose™ 6 Fast Flow Available on GSA/VA Contract for Federal Government customers only.

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.   GE Healthcare Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Sartorius™ Sartoclear Dynamics™: Lab P15

Sartoclear Dynamics™: Lab P15 Cell Harvesting of up to 15 mL Mammalian Cell Cultures in One Step

GE Healthcare StrepTrap™ HP Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

Purify Strep(II)-tagged recombinant proteins by affinity chromatography. GE Healthcare Life Sciences™ StrepTrap™ HP Prepacked Columns are packed with a robust and stable Sepharose™ based medium which results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also high.

GE Healthcare Tricorn Columns Available on GSA/VA Contract for Federal Government customers only.

Tricorn columns are compatible with high flow rates and are designed for high-performance media such as Sepharose, Superdex, and SOURCE.

Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide

Tricosa-peptide for competitively eluting DYKDDDK-tagged fusion proteins from immobilized supports

Thermo Scientific™ Low Protein Binding Collection Tubes, 1.5 mL

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL).

Sartorius™ Sartoclear Dynamics™: Lab V

For simultaneous clarification and sterile filtration of 50 mL to 1 L mammalian cell cultures in one step without centrifugation.

GE Healthcare PD MidiTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL.  GE Healthcare Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

GE Healthcare HiTrap™ Capto™ Adhere Columns Available on GSA/VA Contract for Federal Government customers only.

Designed to remove contaminants in post-protein A purification

CHAPS (White Crystalline Powder), Fisher BioReagents™ Available on GSA/VA Contract for Federal Government customers only.

CAS: 75621-03-3 Molecular Formula: C32H58N2O7S Molecular Weight (g/mol): 614.883 InChI Key: UMCMPZBLKLEWAF-GBSSQDIOSA-N Synonym: 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate PubChem CID: 134129639 IUPAC Name: 3-[dimethyl-[3-[[(4R)-4-[(3R,5S,7R,8R,9R,10S,12S,13R,14R,17S)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]propyl]azaniumyl]propane-1-sulfonate SMILES: CC(CCC(=O)NCCC[N+](C)(C)CCCS(=O)(=O)[O-])C1CCC2C1(C(CC3C2C(CC4C3(CCC(C4)O)C)O)O)C

Sodium Dodecyl Sulfate (SDS), Micropellets, Fisher BioReagents Available on GSA/VA Contract for Federal Government customers only.

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

GE Healthcare HiTrap™ Protein L Prepacked Columns

For capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs

GE Healthcare Ni Sepharose™ High Performance Media Available on GSA/VA Contract for Federal Government customers only.

Precharged with Ni2+ for optimal purification of histidine-tagged proteins.  GE Healthcare Lifescience™ Ni Sepharose™ High Performance Media shows high binding capacity and compatibility.

GE Healthcare HiTrap™ Chelating HP Columns Available on GSA/VA Contract for Federal Government customers only.

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. GE Healthcare Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

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