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Cytiva Butyl Sepharose™ 4 Fast Flow Chromatography Media
Used for capture and intermediate purification of larger proteins
| Matrix | Based on cross-linked 4% agarose |
|---|---|
| Product Type | Butyl Sepharose 4 Fast Flow Media |
Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
| Particle Size | 90μm |
|---|---|
| Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
| Flow Rate | 150 to 600cm/hr. |
| Content And Storage | 4° to 30°C |
| pH Range | 2 to 12 |
| Product Type | Ni Sepharose excel chromato graphy media |
| dimMatrix | Ni-Sepharose |
| Support Type | 6% Highly Cross-linked Agarose |
Novex™ Protein A - Sepharose™ 4B
Protein A - Sepharose™ 4B
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Stationary Phase | Protein A |
|---|---|
| Form | Suspension |
| Product Type | Protein A Sepharose |
| Column Type | Affinity |
| Product Line | Sepharose |
Novex™ Recombinant Protein A-Sepharose™ 4B
Recombinant Protein A-Sepharose™ 4B
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Stationary Phase | Protein A |
|---|---|
| Form | Suspension |
| Product Type | Protein A Sepharose |
| Column Type | Affinity |
Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
| Particle Size | 90μm |
|---|---|
| Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
| Flow Rate | 600 cm/hr. |
| Height (Metric) | 250mm |
| Content And Storage | 4° to 30°C |
| pH Range | 3 to 12 |
| Product Type | Ni Sepharose 6 FF chromatography media |
| dimMatrix | Ni-Sepharose |
| Support Type | 6% Cross-linked Agarose |
| Max. Pressure | 1 bar |
| Height (English) | 9.84 in. |
Novex™ Recombinant Protein G - Sepharose™ 4B
Recombinant Protein G - Sepharose™ 4B
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Stationary Phase | Protein G |
|---|---|
| Form | Suspension |
| Product Type | Protein G Sepharose |
| Column Type | Affinity |
| Product Line | Sepharose |
Cytiva Protein A and Protein G Mag Sepharose™
Simplify enrichment of target proteins by immunoprecipitation or pull-down applications
| Particle Size | 37 to 100 μm |
|---|---|
| Content And Storage | 4°C to 8°C |
| Matrix | Paramagnetic spherical, highly cross linked agarose particles |
Cytiva CNBr-Activated Sepharose™ Medium
Use for coupling antibodies or other large proteins containing -NH2 groups to the Sepharose media. CNBr-Activated Sepharose™ Medium is a pre-activated media perfect for custom affinity applications.
Cytiva Protein G Sepharose™ 4 Fast Flow Media
Rapid processing of large volumes, recoveries of 70 to 90%. Cytiva Protein G Sepharose™ 4 Fast Flow Media is recombinant protein G coupled to Sepharose 4 Fast Flow.
| Particle Size | 90 μm |
|---|---|
| Content And Storage | 2°C to 8°C |
| Matrix | 4% Cross Linked Agarose |
| Ligand | Recombinant Protein G lacking albumin binding region |
| Ligand Concentration | 2mg Protein G/mL Medium |
| pH | 3 to 9 |
| For Use With (Application) | Used in a range of diagnostic and research applications |
Cytiva HiTrap™ Q Sepharose Fast Flow IEX Columns
Use for small-scale protein purifications. Cytiva Life Sciences™ HiTrap™ Q Sepharose Fast Flow IEX Columns are strong anion exchangers for rapid separations either alone or connected in series.
| Content And Storage | 4°C to 30°C in 20% Ethanol |
|---|---|
| Description | HiTrap Q FF |
Cytiva Streptavidin Sepharose™ High Performance Medium
Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.
Cytiva GammaBind Plus Sepharose
Binds to all IgG subclasses. Cytiva GammaBind Plus Sepharose is used for lab-scale affinity purification of IgG from a large variety of species and has better binding to rat and mouse IgG subclasses compared to standard GammaBind Sepharose.
| Particle Size | 90 μm, d50v is the median particle size of the cumulative volume distribution. |
|---|---|
| Flow Rate | >130 cm/h |
| Content And Storage | 2°C to 8°C, 20% Ethanol |
| Ligand | Recombinant GammaBind G type 3 lacking albumin-binding region |
Cytiva NHS Mag Sepharose™
NHS Mag Sepharose magnetic beads for convenient enrichment of selected proteins using pull-down sample preparation techniques
| Particle Size | 37 to 100 μm |
|---|---|
| Content And Storage | 4°C to 8°C |
| Matrix | Paramagnetic spherical, highly cross linked agarose particles |
| Bead Type | NHS Mag Sepharose |
| Ligand | N-hydroxysuccinimide |
| Ligand Concentration | 8 to 14μM/mL |