Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, low ROX

Description

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

• High Sensitivity & Specificity - The master mixes contain the patented hot start enzyme Thermo-Start DNA Polymerase
• Optimized buffer containing SYBR Green
• Reproducibility - All master mixes undergo extensive QC to ensure batch to batch consistency
• Broad dynamic range

Specifications

• Concentration: 2X
• Content And Storage: Contains:
  • 1 x 5 mL ABsolute QPCR SYBR Green Low ROX Mix (2X)
  • 100 μL MgCl₂ (1M)
  
  Store at -20°C
• Detection Method: SYBR
• PCR Method: qPCR
• Polymerase: Taq DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
• Product Line: ABsolute
• Product Type: Real Time PCR SYBR Master Mix
• Quantity: 400 reactions
• Sufficient For: 400 Reactions of 25 μL Each
• For Use With (Application): Gene Expression
• Compatibility: qPCR Cyclers
• No. of Reactions: 400 Reactions
• Sample Type: DNA, dsDNA
• Volume: 5 mL

For Research Use Only. Not for use in diagnostic procedures.