Thermo Scientific™ ABsolute™ qPCR Mixes

ABsolute qPCR Mixes are optimized for use with hybridization probes.

Catalog Number	Mfr. No.	Quantity
CM-205A	Thermo Scientific™ CM205A	10 x 5mL vials; 2000 x 50μL rxns

Description

ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.

Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.

Highlights

• High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
• Optimised buffer - For maximal performance in multiplex reactions

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Includes

• Absolute qPCR Mix (no ROX) contains 2X Master Mix and no passive reference dye
• Absolute qPCR ROX (500 nm) Mix contains 2X Master Mix supplemented with high ROX concentration
• Absolute qPCR Low ROX Mix contains 2X Master Mix supplemented with low ROX concentration
• Absolute qPCR Capillary Mix contains 2X Master Mix optimized for capillary instruments

Hazardous: No
Storage Condition: -20°C
Storage notes: Note that ROX dye is light sensitive; exposure should be minimized.

Gene expression analysis, siRNA validation, and genotyping.

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.