alpha-Smooth Muscle Actin Mouse, Clone: SPM332, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 4 publications

Catalog Number	Mfr. No.	Quantity
NBP232808F	Novus Biologicals NBP2328080.02MG	0.02 mg
NBP232808A	Novus Biologicals NBP2328080.1MG	0.1 mg
NBP232808B	Novus Biologicals NBP2328080.2MG	0.2 mg

Description

alpha-Smooth Muscle Actin Monoclonal specifically detects alpha-Smooth Muscle Actin in Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Baboon, Chimpanzee, Monkey, Rabbit, Sheep samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

• Antigen: alpha-Smooth Muscle Actin
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Baboon, Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Primate, Rabbit, Sheep
• Gene Accession No.: P62736
• Gene ID (Entrez): 59
• Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 42 kDa

• Clone: SPM332
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Extracellular Matrix, Phospho Specific, Stem Cell Markers
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: AAT6, actin, alpha 2, smooth muscle, aorta, actin, aortic smooth muscle, ACTVS, alpha 2 actin, alpha SMA, alpha smooth muscle actin, alpha-actin-2, alpha-SMA, a-sma, Cell growth-inhibiting gene 46 protein, growth-inhibiting gene 46, sma, smooth muscle actin
• Gene Symbols: ACTA2
• Isotype: IgG2a κ
• Purification Method: Protein A purified
• Test Specificity: Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.