anti-CD3zeta/T Cell Receptor zeta Ab-8, Clone: 6B10, Epredia™ LabVision

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Conjugate	Concentration	Quantity
MS1289P0	Epredia MS1289P0	Unconjugated	200μg/mL	100 μL
MS1289P1	Epredia MS1289P1	Unconjugated	200μg/mL	500 μL
MS1289P	Epredia MS1289P	Unconjugated	200μg/mL	1 mL
MS1289P1ABX	Epredia MS1289P1ABX	Unconjugated	1mg/mL	100 μL
MS1289PABX	Epredia MS1289PABX	Unconjugated	1mg/mL	200 μL

Description

CD3 consists of five different polypeptide chains (designated as γ, δ, ε, ζ, and η). The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). It is involved in signal transduction to the T cell interior following antigen recognition, which culminates in cytokine release, cellular proliferation.. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Host Species: Mouse
Clone: 6B10
Isotype: IgG1
Cross Reactivity: Human, Mouse, and Rat. Others-not tested.
Epitope: aa 36-54 of T Cell Receptor zeta Chain
Immunogen: Synthetic peptide corresponding to aa 36-54 of T Cell Receptor ζ Chain.
Molecular Weight: ∽20kDa
Positive Control: Jurkat cells.
Cellular Localization: Cell membrane

Ab-8 reacts with phosphorylated and unphosphorylated forms of of T Cell Receptor zeta Chain. It co-immunoprecipitates the zeta chain associated tyrosine kinase ZAP-70

Immunoprecipitation, Western Blotting

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly

Specifications

• Antigen: CD3ζ/T Cell Receptor ζ Ab-8
• Conjugate: Unconjugated
• Host Species: Mouse
• Immunogen: Synthetic peptide corresponding to aa 36-54 of T Cell Receptor ζ Chain.
• Primary or Secondary: Primary

• Classification: Monoclonal
• Form: Purified
• Target Species: Human, Mouse, Rat
• Isotype: IgG1
• Purification Method: Jurkat cells